Western blot abreview for Anti-CAB3 antibody

Good
Abreviews
Application
Western blot
Sample
Rat Recombinant protein (recombinant protein from e.Coli)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
recombinant protein from e.Coli
Blocking step
Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 19°C · Diluent: PBS-tween (0.1%)
Dilution
1/500

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Goat anti-rabbit anitbody HRP conjugated
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
45 second(s)
Bands
Specific: 28 kDa

Additional data

Additional Notes
Protein analysed was CavBeta3-SH3 domain fused with a solubility marker. Therefore, the estimated molecular weight is 28kDa. protein marker is SeeBlue plus2 run on 4-12% Bis-Tris gel in MOPS buffer.

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Submitted Oct 23 2008

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