Product nameAnti-CAB39/MO25 antibody [EP1680Y]
See all CAB39/MO25 primary antibodies
DescriptionRabbit monoclonal [EP1680Y] to CAB39/MO25
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human CAB39/MO25 (C terminal). The exact sequence is proprietary.
- HeLa cell lysate, human brain tissue, or HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab51132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/20000. Detects a band of approximately 35 kDa (predicted molecular weight: 40 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
We strongly recommend that customers perform an antigen retireval step.
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/1000 - 1/10000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionTogether with the STE20-related adaptor-alpha (STRAD alpha) pseudo kinase, forms a regulatory complex capable of stimulating the activity of STK11.
Sequence similaritiesBelongs to the Mo25 family.
- Information by UniProt
- Cab39 antibody
- CAB39_HUMAN antibody
- Calcium binding protein 39 antibody
Anti-CAB39/MO25 antibody [EP1680Y] (ab51132) at 1/20000 dilution + HeLa cell lysate at 10 µg
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Overlay histogram showing HeLa cells stained with ab51132 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51132, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Ab51132 (1:100) staining human CAB39/MO25 in human brain tissue by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescent staining of HeLa cells using ab51132 (1:100).
This product has been referenced in:
- Li J et al. miR-451 Silencing Inhibited Doxorubicin Exposure-Induced Cardiotoxicity in Mice. Biomed Res Int 2019:1528278 (2019). Read more (PubMed: 31355248) »
- Zeng J et al. PrLZ increases prostate cancer docetaxel resistance by inhibiting LKB1/AMPK-mediated autophagy. Theranostics 8:109-123 (2018). Read more (PubMed: 29290796) »