Overview

  • Product name

  • Description

    Rabbit polyclonal to CABP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human CABP aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab108465)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Mouse brain; Mouse cerebellum; Mouse hippocampus; Mouse spinal cord.

Properties

Applications

Our Abpromise guarantee covers the use of ab93853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Target

  • Function

    Modulates calcium-dependent activity of inositol 1,4,5-triphosphate receptors (ITPRs). Inhibits agonist-induced intracellular calcium signaling. Enhances inactivation and does not support calcium-dependent facilitation of voltage-dependent P/Q-type calcium channels. Causes calcium-dependent facilitation and inhibits inactivation of L-type calcium channels by binding to the same sites as calmodulin in the C-terminal domain of CACNA1C, but resulting in an opposit effects on channel function. Suppresses the calcium-dependent inactivation of CACNA1D (By similarity). Inhibits TRPC5 channels. Prevents NMDA receptor-induced cellular degeneration.
  • Tissue specificity

    Retina and brain. Somatodendritic compartment of neurons. Calbrain was found exclusively in brain where it is abundant in the hippocampus, habenular area in the epithalamus and in the cerebellum.
  • Sequence similarities

    Contains 4 EF-hand domains.
  • Domain

    EF-1 binds magnesium constitutively under physiological conditions, EF-3 and EF-4 bind calcium cooperatively and EF-2 binds neither calcium nor magnesium.
  • Post-translational
    modifications

    Phosphorylated. The phosphorylation regulates the activity.
  • Cellular localization

    Cytoplasm > cell cortex. Cell membrane. S-CaBP1 is localized at or near the plasma membrane; Cytoplasm > cytoskeleton. Cytoplasm > perinuclear region. Cell membrane. Golgi apparatus. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. L-CaBP1 is associated most likely with the cytoskeletal structures, whereas S-CaBP1 is localized at or near the plasma membrane and Cytoplasm > cytoskeleton. L-CaBP1 is associated most likely with the cytoskeletal structures.
  • Information by UniProt
  • Database links

  • Alternative names

    • CABP 1 antibody
    • CaBP1 antibody
    • CABP1_HUMAN antibody
    • Calbrain antibody
    • Calcium binding protein 1 antibody
    • Calcium binding protein 5 antibody
    • Calcium-binding protein 1 antibody
    • Caldendrin antibody
    • HCALB BR antibody
    • HCALB_BR antibody
    see all

Images

  • All lanes : Anti-CABP antibody (ab93853) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Cerebellum Mouse Tissue Lysate
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : Spinal Cord (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 25 kDa
    Additional bands at: 85 kDa, 95 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes

References

ab93853 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (primary hippocampal neurons)
Specification
primary hippocampal neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - ice-cold methanol (optional)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 15 2012

Application
Western blot
Sample
Mouse Cell lysate - whole cell (primary hippocampal neurons)
Loading amount
20 µg
Specification
primary hippocampal neurons
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 14 2012

Answer

Vielen Dank für Ihre Anfrage.

Der Anti-CABP-Antikörper ab93853wurde nach einem Standard-Westernblot-Protokoll getestet, das auch auf unserer Webseite zu finden ist(siehe Anhang und https://www.abcam.com/index.html?pageconfig=popular_protocols).

In unseren Laboren wird routinemäβig mit 5% BSA blockiert. Je nach Ergebnis werden den Antikörperverdünnungen dann bis zu 0,5% BSA zugefügt. Wir haben die Erfahrung gemacht, dass die meisten Antikörper mit BSA ein gleichmäβigeres Signal ergeben. Es spricht jedoch auch nichts dagegen, mit 5% Milch zu blockieren; sollte hier der Hintergrund etwas stärker ausfallen, würden wir dann zu BSA raten.

Ich hoffe, dies hilft Ihnen weiter.

Falls Sie jedoch noch andere Probleme mit ab93853 haben, können wir gerne versuchen konkret zu helfen: Ich habe daherunseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Fragebogens erhalten wir alle nötigen Informationen über Ihre Proben und Ihr Protokoll. Sobald Sie dieses Formular und ggf. eine Abbildung an uns zurückgeschickt haben, werden wir uns Ihr Protokoll ansehen und möglichst Veränderungsvorschläge machen, die Ihre Ergebnisse verbessern werden. Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben und er innerhalb der letzten sechs Monate gekauft wurde, werden wir Ihnen gerne einen Ersatz oder eine Gutschrift schicken (https://www.abcam.com/Abpromise).

Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Answer

Thank you for your email.
DISCOUNT CODE: xxx for ab103423
DISCOUNT CODE: xxx for ab93853
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab103423 and ab93853 in ICC/IF. Each code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit each an Abreview for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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