Overview

  • Product name

    Anti-CACNA1A antibody - N-terminal
    See all CACNA1A primary antibodies
  • Description

    Rabbit polyclonal to CACNA1A - N-terminal
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Rabbit, Human
  • Immunogen

    Synthetic peptide corresponding to Human CACNA1A aa 430-447 (N terminal).
    Sequence:

    KTDLLNPEEAEDQLADIA


    Database link: O00555

  • Positive control

    • Rat brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab191140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells.
WB Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 282 kDa (predicted molecular weight: 282 kDa).

Target

  • Relevance

    Cav2.1 is a voltage-sensitive calcium channels (VSCC) which belongs to the calcium channel alpha-1 subunit family. Cav2.1 mediates the entry of calcium ions into excitable cells and is also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. Cav2.1 (isoform alpha-1A) gives rise to P and/or Q-type calcium currents. Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity.
  • Cellular localization

    Cell Membrane
  • Database links

  • Alternative names

    • APCA antibody
    • BI antibody
    • Brain calcium channel 1 antibody
    • Brain calcium channel I antibody
    • CACH4 antibody
    • CACN3 antibody
    • CACNA1A antibody
    • CACNL1A4 antibody
    • Calcium channel alpha 1A subunit antibody
    • Calcium channel L type alpha 1 polypeptide antibody
    • Calcium channel L type alpha 1 polypeptide isoform 4 antibody
    • Calcium channel voltage dependent, P/Q type alpha 1A subunit antibody
    • CAV2.1 antibody
    • EA2 antibody
    • FHM antibody
    • HPCA antibody
    • MHP antibody
    • MHP1 antibody
    • RAT brain class A antibody
    • RBA-I antibody
    • SCA6 antibody
    • Voltage dependent P/Q type calcium channel alpha 1A subunit antibody
    • Voltage gated calcium channel alpha subunit Cav2.1 antibody
    see all

Images

  • Overlay histogram showing U-87 cells stained with ab191140 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with ab191140 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

  • Overlay histogram showing U20S cells stained with ab191140 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with ab191140 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

  • Anti-CACNA1A antibody - N-terminal (ab191140) at 0.5 µg/ml + Rat Brain Tissue Lysate at 40 µg

    Predicted band size: 282 kDa
    Observed band size: 282 kDa

References

ab191140 has not yet been referenced specifically in any publications.

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