Anti-CACNA1C antibody (ab140760)
Key features and details
- Rabbit polyclonal to CACNA1C
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CACNA1C antibody
See all CACNA1C primary antibodies -
Description
Rabbit polyclonal to CACNA1C -
Host species
Rabbit -
Specificity
BLAST analysis of the peptide immunogen showed no homology with other Human proteins. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Monkey, Gorilla, Common marmoset -
Immunogen
Synthetic peptide corresponding to Human CACNA1C (internal sequence). Synthetic 16 amino acid peptide from internal region of human CACNA1C
Database link: Q13936 -
Positive control
- Human small intestine tissue.
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General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function. -
Tissue specificity
Expressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle. -
Involvement in disease
Timothy syndrome
Brugada syndrome 3 -
Sequence similarities
Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily. -
Domain
Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel. -
Post-translational
modificationsPhosphorylation by PKA activates the channel. -
Cellular localization
Membrane. Cell membrane. The interaction between RRAD and CACNB2 regulates its trafficking to the cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 100406735 Common marmoset
- Entrez Gene: 775 Human
- Omim: 114205 Human
- SwissProt: Q13936 Human
- Unigene: 118262 Human
- Unigene: 690010 Human
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Alternative names
- alpha-1 polypeptide antibody
- cardiac muscle antibody
- isoform 1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab140760 has not yet been referenced specifically in any publications.