Key features and details
- Goat polyclonal to CACNA1C
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CACNA1C antibody
See all CACNA1C primary antibodies
DescriptionGoat polyclonal to CACNA1C
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey
- Human Heart and Skeletal Muscle lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab81980 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab81980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 249 kDa (predicted molecular weight: 249 kDa).
1 hour primary incubation is recommended for this product.
FunctionVoltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function.
Tissue specificityExpressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle.
Involvement in diseaseTimothy syndrome
Brugada syndrome 3
Sequence similaritiesBelongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily.
DomainEach of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel.
modificationsPhosphorylation by PKA activates the channel.
Cellular localizationMembrane. Cell membrane. The interaction between RRAD and CACNB2 regulates its trafficking to the cell membrane.
- Information by UniProt
- alpha-1 polypeptide antibody
- cardiac muscle antibody
- isoform 1 antibody
ab81980 has been referenced in 3 publications.
- Papp R et al. Genomic upregulation of cardiac Cav1.2a and NCX1 by estrogen in women. Biol Sex Differ 8:26 (2017). PubMed: 28807015
- Papp R et al. Genomic upregulation of cardiac Cav1.2a and NCX1 by estrogen in women. Biol Sex Differ 8:26 (2017). PubMed: 28815011
- Herraiz-Martínez A et al. Ageing is associated with deterioration of calcium homeostasis in isolated human right atrial myocytes. Cardiovasc Res 106:76-86 (2015). PubMed: 25712961