Overview

  • Product name

  • Description

    Goat polyclonal to CACNA1C
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human CACNA1C aa 2201-2213 (C terminal).
    Sequence:

    C-RARGRPSEEELQD

  • Positive control

    • Human Heart and Skeletal Muscle lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab81980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 249 kDa (predicted molecular weight: 249 kDa).

Target

  • Function

    Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function.
  • Tissue specificity

    Expressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle.
  • Involvement in disease

    Timothy syndrome
    Brugada syndrome 3
  • Sequence similarities

    Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily.
  • Domain

    Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
    Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel.
  • Post-translational
    modifications

    Phosphorylation by PKA activates the channel.
  • Cellular localization

    Membrane. Cell membrane. The interaction between RRAD and CACNB2 regulates its trafficking to the cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • alpha-1 polypeptide antibody
    • cardiac muscle antibody
    • isoform 1 antibody
    • L type antibody
    • CAC1C_HUMAN antibody
    • CACH 2 antibody
    • CACH2 antibody
    • CACN 2 antibody
    • CACN2 antibody
    • CACNA1C antibody
    • CACNL1A1 antibody
    • Calcium channel antibody
    • Calcium channel cardic dihydropyridine sensitive alpha 1 subunit antibody
    • Calcium channel L type alpha 1 polypeptide isoform 1 cardiac muscle antibody
    • Calcium channel voltage dependent L type alpha 1C subunit antibody
    • CaV1.2 antibody
    • CCHL1A1 antibody
    • DHPR alpha 1 antibody
    • DHPR alpha 1 subunit antibody
    • LQT8 antibody
    • TS antibody
    • Voltage dependent L type calcium channel alpha 1C subunit antibody
    • Voltage dependent L type calcium channel subunit alpha 1C antibody
    • Voltage gated calcium channel alpha subunit Cav1.2 antibody
    • Voltage gated calcium channel subunit alpha Cav1.2 antibody
    • Voltage gated L type calcium channel Cav1.2 alpha 1 subunit, splice variant 10* antibody
    • Voltage-dependent L-type calcium channel subunit alpha-1C antibody
    • Voltage-gated calcium channel subunit alpha Cav1.2 antibody
    see all

Images

  • Anti-CACNA1C antibody (ab81980) at 0.5 µg/ml + Human Heart lysate in RIPA buffer at 35 µg

    Predicted band size: 249 kDa
    Observed band size: 249 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence.

References

This product has been referenced in:

See all 3 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Answer

Thank you for contacting Abcam.
I have processed your request to receive ab81980 as a replacement for ab84814 and your new order number is ********.
I hope that this new antibody is successful and if there is anything else I can help you with, please let me know.

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Answer

Thank you for reply.
Out of either ab81980 or ab8109, I would recommend ab81980, as we have a western blot image for that one on our website. We do not have any more data for either antibody, although both are guaranteed to work in western blot on human samples.
I look forward to your reply and helping you resolve this issue.

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Answer

Thank you for your reply.
I am sorry that the protocol tips did not prove to be successful. Under our Abpromise we do guarantee this antibody for 6 months to work in western blot on human samples. Since we have not been able to resolve this issue for you, then I would be happy to send you a replacement antibody as requested..
To do that, I need to have your original order information. I believe I have found that information, could you confirm that the product was ordered on 9th Nov 2011 by Bethann Gabris. If this information is incorrect, could you send me the correct information? Also, I can send you either ab81980 or ab81095, just let me know which one you would like to receive.
I look forward to your reply and resolving this issue for you.

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Answer

Thank you for contacting Abcam. I am sorry that you are having problems with ab84814 in western blot. I have looked over the images and protocol information that you sent and there are a few protocol changes that I would suggest that may help to reduce the background that you are seeing: 1 - Switch to a 5% BSA block rather than 5% Milk, as BSA is a little better a blocking non-specific binding. 2 - Increase the number of wash steps you are doing (up 5 washes, 5 minutes each) and add 0.05% Tween 20 to the wash buffer, if you have not done so already. 3 - After the secondary incubation, wash the membrane for 30 minutes in a 0.5M NaCl. Then rinse the membrane thoroughly in dH20 and develop as normal. If these protocol tips do not work then I would be happy to send you a replacement antibody, as under our Abpromise we do guarantee this antibody to work in western blot on human samples. I look forward to your reply and helping you resolve these issues.

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Answer

Thank you for contacting Abcam. Under our Abpromise we guarantee all of our antibodies to work as stated on the datasheet, if they do work as stated and we cannot offer any assistance to resolve the matter, then we would either replace the antibody or give a refund. Both ab81980 and ab84814 are guaranteed to work in western blot on human samples. I would recommended ab84814, even though the western blot image may not be the cleanest. If there is anything else I can help you with, please let me know.

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