Overview

  • Product name

    Anti-CACNA1C antibody [EPR22625-61]
    See all CACNA1C primary antibodies
  • Description

    Rabbit monoclonal [EPR22625-61] to CACNA1C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details
    Unsuitable for: IP or WB
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse CACNA1C aa 2100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q01815

  • Positive control

    • IHC-P: Mouse atrium, ventricle and colon tissue. IHC-Fr: Mouse cardiac muscle and cerebrum tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab234438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500.
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP or WB.
  • Target

    • Function

      Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function.
    • Tissue specificity

      Expressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle.
    • Involvement in disease

      Timothy syndrome
      Brugada syndrome 3
    • Sequence similarities

      Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily.
    • Domain

      Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
      Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel.
    • Post-translational
      modifications

      Phosphorylation by PKA activates the channel.
    • Cellular localization

      Membrane. Cell membrane. The interaction between RRAD and CACNB2 regulates its trafficking to the cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • alpha-1 polypeptide antibody
      • cardiac muscle antibody
      • isoform 1 antibody
      • L type antibody
      • CAC1C_HUMAN antibody
      • CACH 2 antibody
      • CACH2 antibody
      • CACN 2 antibody
      • CACN2 antibody
      • CACNA1C antibody
      • CACNL1A1 antibody
      • Calcium channel antibody
      • Calcium channel cardic dihydropyridine sensitive alpha 1 subunit antibody
      • Calcium channel L type alpha 1 polypeptide isoform 1 cardiac muscle antibody
      • Calcium channel voltage dependent L type alpha 1C subunit antibody
      • CaV1.2 antibody
      • CCHL1A1 antibody
      • DHPR alpha 1 antibody
      • DHPR alpha 1 subunit antibody
      • LQT8 antibody
      • TS antibody
      • Voltage dependent L type calcium channel alpha 1C subunit antibody
      • Voltage dependent L type calcium channel subunit alpha 1C antibody
      • Voltage gated calcium channel alpha subunit Cav1.2 antibody
      • Voltage gated calcium channel subunit alpha Cav1.2 antibody
      • Voltage gated L type calcium channel Cav1.2 alpha 1 subunit, splice variant 10* antibody
      • Voltage-dependent L-type calcium channel subunit alpha-1C antibody
      • Voltage-gated calcium channel subunit alpha Cav1.2 antibody
      see all

    Images

    • Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling CACNA1C with ab234438 at 1/500 (0.87 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. Positive staining on mouse cerebrum is observed.

    • Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen mouse cardiac muscle tissue labeling CACNA1C with ab234438 at 1/500 (0.87 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. Positive staining on mouse cardiac atrium myocytes and blood vessels throughout cardiac muscle (PMID: 26109061).

    • Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling CACNA1C with ab234438 at 1/4000 dilution (0.11 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on endothelial cells of blood vessels, muscularis mucosa and muscularis externa in mouse colon is observed. The section was incubated with ab234438 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    • Immunohistochemical analysis of paraffin-embedded mouse ventricle tissue labeling CACNA1C with ab234438 at 1/2000 dilution (0.11 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the endothelial cells of blood vessels in mouse ventricle is observed (PMID: 26109061). The section was incubated with ab234438 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    • Immunohistochemical analysis of paraffin-embedded mouse atrium tissue labeling CACNA1C with ab234438 at 1/4000 dilution (0.11 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on plasma membranes of atrial cardiomyocytes and endothelial cells of blood vessels is observed (PMID: 26109061). The section was incubated with ab234438 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    References

    ab234438 has not yet been referenced specifically in any publications.

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