Product nameAnti-CACNA1C antibody [L57/46]
See all CACNA1C primary antibodies
DescriptionMouse monoclonal [L57/46] to CACNA1C
Tested applicationsSuitable for: ICC/IF, IHC-P, IHC-Fr, IP, WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Dog, Human
Fusion protein corresponding to Rabbit CACNA1C aa 1507-1733 (C terminal).
- WB: Cell lysates prepared from DHPR alpha 1 transfected CHO cells. IHC-P: Human hippocampus tissue; Mouse brain tissue. ICC/IF: SK-N-BE cells. Flow Cyt: SH-SY5Y cells. IHC-Fr: Rabbit heart tissue.
The clone number has been updated from S57-46 to L57/46, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab84814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.1 - 10 µg/ml. Use at 0.1-1.0µg/mL (perox), 1.0-10µg/mL (IF).|
|IHC-Fr||Use a concentration of 0.1 - 10 µg/ml. Use at 0.1-1.0µg/mL (perox), 1.0-10µg/mL (IF).|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 240 kDa. If results are poor, use lysate without boiling, heat at 37°C for 15 minutes.|
|Flow Cyt||Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionVoltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function.
Tissue specificityExpressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle.
Involvement in diseaseTimothy syndrome
Brugada syndrome 3
Sequence similaritiesBelongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily.
DomainEach of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel.
modificationsPhosphorylation by PKA activates the channel.
Cellular localizationMembrane. Cell membrane. The interaction between RRAD and CACNB2 regulates its trafficking to the cell membrane.
- Information by UniProt
- alpha-1 polypeptide antibody
- cardiac muscle antibody
- isoform 1 antibody
All lanes : Anti-CACNA1C antibody [L57/46] (ab84814) at 1 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from DHPR alpha 1 transfected CHO cells
ab84814 staining CACNA1C in human hippocampus tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin embedded tissue sections). Tissue underwent heat mediate fixation in microwave and in citrate buffer. The primary antibody was used at 1/100 dilution. A Fluorophore conjugated goat anti-mouse was used as secondary at 1/50 dilution.
ab84814 staining CACNA1C in mouse brain tissue section by immunohistochemistry (Bouin's fixed paraffin embedded tissue section. Tissue underwent heat mediated fixation in microwave and in citrate buffer. The primary antibody was used at 1/100 dilution. A HRP conjugated secondary was used at 10 dilution.
ab84814 staining CACNA1C in rabbit heart tissue by Immunohistochemistry (Frozen sections).Tissue was fixed with paraformaldehyde, permeabilized using Triton X-100 and then incubated with ab84814 at a 1/100 dilution for 12 hours at 4°C. The secondary used was an AlexaFluor® 555 conjugated goat anti-mouse monoclonal, used at a 1/500 dilution.
Overlay histogram showing SH-SY5Y cells stained with ab84814 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84814, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.
Formaldehyde-fixed SK-N-BE cells stained for CACNA1C (green) using ab84814 at 1/100 dilution in ICC/IF.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/200 dilution for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain (A); DAPI (B, blue) nuclear stain at 1/1000, 1/5000 for 60 minutes at room temperature, 5 minutes at room temperature.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab84814 has been referenced in 12 publications.
- Lai YJ et al. Estrogen receptor a promotes Cav1.2 ubiquitination and degradation in neuronal cells and in APP/PS1 mice. Aging Cell N/A:e12961 (2019). PubMed: 31012223
- Gao Y et al. Calcium Receptor and Nitric Oxide Synthase Expression in Circular Muscle of Lower Esophagus from Patients with Achalasia. Chin Med J (Engl) 131:2882-2885 (2018). PubMed: 30511698
- Zhang Y et al. REDUCING TOXICITY AND INCREASING EFFICIENCY: ACONITINE WITH LIQUIRITIN AND GLYCYRRHETINIC ACID REGULATE CALCIUM REGULATORY PROTEINS IN RAT MYOCARDIAL CELL. Afr J Tradit Complement Altern Med 14:69-79 (2017). WB ; Rat . PubMed: 28638869
- Doshina A et al. Cortical cells reveal APP as a new player in the regulation of GABAergic neurotransmission. Sci Rep 7:370 (2017). PubMed: 28337033
- Papp R et al. Genomic upregulation of cardiac Cav1.2a and NCX1 by estrogen in women. Biol Sex Differ 8:26 (2017). PubMed: 28807015