Immunohistochemistry (Frozen sections) abreview for Anti-DHPR alpha 1 antibody [S57-46]

Inconclusive
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Specification
heart
Fixative
Paraformaldehyde
Permeabilization
Yes - triton-X 100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C

Other product details

Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+BSA+serum

Secondary antibody

Name
Non-Abcam antibody was used: goat anti mouse IgG2b488
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
The background is noisy. No specific staining is seen.
Abcam response
Thank you for your valuable feedback. We are sorry to hear that this antibody has not given you good results in Immunohistochemistry frozen sections (IHC-Fr) with mouse sample. This antibody has not been tested in this application and species before, and it is possible that it is not suitable for use. However, we would be unable to confirm this without further testing of the antibody in the application.

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Submitted Feb 17 2012

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