Immunohistochemistry (Frozen sections) abreview for Anti-DHPR alpha 1 antibody [S57-46]

Inconclusive
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (atrium)
Specification
atrium
Fixative
Paraformaldehyde
Permeabilization
Yes - triton-X 100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C

Other product details

Dilution
1/50
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+BSA+serum

Secondary antibody

Name
Non-Abcam antibody was used: goat anti mouse IgG2b488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
only see non-specifc binding
Abcam response
Thank you for submitting your Abreview. We are very sorry to hear that the antibody did not perform as expected in this tested species and application. We are in touch with the customer to resolve this problem according to our Abpromise guarantee (https://www.abcam.com/abpromise) and to the customer’s satisfaction.

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Verified customer

Submitted Apr 17 2012

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