Immunohistochemistry (Frozen sections) abreview for Anti-DHPR alpha 1 antibody [S57-46]

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Horse Tissue sections (Skeletal muscles)
Specification
Skeletal muscles
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Other product details

Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: in blocking buffer

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-mouse from JacksonImmunoresearch
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/200

Additional data

Additional Notes
The antibody worked well on horse skeletal muscle cryostat sections. However, there are many non-specific spots.

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Submitted Jul 17 2012

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