Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11822] to CACNA1F
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Product nameAnti-CACNA1F antibody [EPR11822]
See all CACNA1F primary antibodies
DescriptionRabbit monoclonal [EPR11822] to CACNA1F
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
- Y79 cells, A673 and Y79 cell lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 221 kDa.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceCACNA1F (Calcium channel, voltage-dependent, L type, alpha 1F subunit) is a subunit of a voltage-dependent calcium channel complex. Voltage-gated calcium channels mediate the entry of calcium ions into excitable cells. CACNA1F gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the "high-voltage activated" (HVA) group and are blocked by dihydropyridines (DHP).
Cellular localizationMembrane; Multi-pass membrane protein.
- Aland island eye disease (Forsius Eriksson ocular albinism, ocular albinism type 2) antibody
- CAC1F_HUMAN antibody
- CACNA 1F antibody
All lanes : Anti-CACNA1F antibody [EPR11822] (ab171968) at 1/1000 dilution
Lane 1 : A673 cell lysate
Lane 2 : Y79 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 221 kDa
Flow cytometric analysis of Y79 cells, labeling CACNA1F using ab171968 at a 1/100 dilution (red) or a rabbit IgG negative control (green).
ab171968 has been referenced in 1 publication.
- Huang JJ et al. Functional expression of the Ca2+ signaling machinery in human embryonic stem cells. Acta Pharmacol Sin 38:1663-1672 (2017). PubMed: 28713161