Overview

  • Product name

    Anti-CACNA1G antibody [N178A/9]
    See all CACNA1G primary antibodies
  • Description

    Mouse monoclonal [N178A/9] to CACNA1G
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein, corresponding to C terminal amino acids 2052-2172 of Mouse CACNA1G (NP_001106284.1).

  • Positive control

    • WB: Rat brain membrane tissue lysate. ICC/IF: SK-N-BE cells.
  • General notes

    The clone number has been updated from S178A-9 to N178A/9, both clone numbers name the same antibody clone.

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    N178A/9
  • Isotype

    IgG1

Applications

Our Abpromise guarantee covers the use of ab134269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 262 kDa. 1µg/ml of ab134269 is sufficient for detection of CACNA1G in 20µg of Rat brain membrane lysate.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100.

Target

  • Relevance

    Voltage activated calcium channels can be distinguished based on their voltage dependence, deactivation, and single channel conductance. Low voltage activated calcium channels are referred to as 'T' type because their currents are both transient, owing to fast inactivation, and tiny, owing to small conductance. T type channels are thought to be involved in pacemaker activity, low threshold calcium spikes, neuronal oscillations and resonance, and rebound burst firing.
  • Cellular localization

    Membrane; Multipass membrane protein.
  • Database links

  • Alternative names

    • calcium channel voltage dependent alpha 1G subunit antibody
    • calcium channel voltage dependent T type alpha 1G subunit antibody
    • calcium channel voltage dependent T type alpha1G subunit antibody
    • CaV T1 antibody
    • Cav3 1 antibody
    • cav3 1c antibody
    • KIAA1123 antibody
    • MGC117234 antibody
    • NBR13 antibody
    • voltage dependent calcium channel alpha 1G subunit isoform 11 antibody
    • voltage dependent T type calcium channel subunit alpha 1G antibody
    • Voltage gated calcium channel subunit alpha Cav3 1 antibody
    see all

Images

  • Anti-CACNA1G antibody [N178A/9] (ab134269) at 1/1000 dilution + Rat brain membrane tissue lysate

    Secondary
    Goat anti-mouse IgG HRP

    Developed using the ECL technique.

    Predicted band size: 262 kDa

  • 4% formaldehyde-fixed SK-N-BE cells stained for CACNA1G (green) using ab134269 at 1/100 dilution in ICC/IF.

    Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/200 for 60 min at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000; 1/5000 for 60 min room temperature, 5 min room temperature.

    A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab134269. (D) Composite.

References

This product has been referenced in:

  • Kim JW  et al. T-Type Calcium Channels Are Required to Maintain Viability of Neural Progenitor Cells. Biomol Ther (Seoul) 26:439-445 (2018). Read more (PubMed: 29463073) »
See 1 Publication for this product

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