Overview

  • Product name

  • Description

    Rabbit polyclonal to CACNA1H
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to the N terminal domain of Human CACNA1H. (Uniprot: O95180)

Properties

Applications

Our Abpromise guarantee covers the use of ab128251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 259 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1H gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes.
  • Tissue specificity

    Expressed in kidney, liver, heart, brain. Isoform 2 seems to be testis-specific.
  • Involvement in disease

    Defects in CACNA1H are a cause of susceptibility to idiopathic generalized epilepsy type 6 (IGE6) [MIM:611942]. IGE is characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain. IGE6 is a polygenic and multifactorial disease.
  • Sequence similarities

    Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1H subfamily.
  • Domain

    Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
  • Post-translational
    modifications

    In response to raising of intracellular calcium, the T-type channels are activated by CaM-kinase II.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha1 3.2 antibody
    • Alpha13.2 antibody
    • CAC1H_HUMAN antibody
    • CACNA 1H antibody
    • CACNA1 H antibody
    • CACNA1 HB antibody
    • Cacna1h antibody
    • CACNA1HB antibody
    • Calcium channel alpha13.2 subunit antibody
    • Calcium channel voltage dependent T type alpha 1H subunit antibody
    • Calcium channel, voltage-dependent, T type, alpha 1Hb subunit antibody
    • Cav 3.2 antibody
    • Cav T.2 antibody
    • Cav3.2 antibody
    • CavT.2 antibody
    • EIG 6 antibody
    • EIG6 antibody
    • Low voltage activated calcium channel alpha 13.2 subunit antibody
    • Low voltage activated calcium channel alpha1 3.2 subunit antibody
    • Low-voltage-activated calcium channel alpha1 3.2 subunit antibody
    • MNCb 1209 antibody
    • T type Cav3.2 antibody
    • Voltage dependent t type calcium channel alpha 1H subunit antibody
    • Voltage gated calcium channel alpha subunit Cav 3.2 antibody
    • Voltage gated calcium channel alpha subunit Cav T.2 antibody
    • Voltage gated calcium channel alpha subunit Cav3.2 antibody
    • Voltage gated calcium channel alpha subunit CavT.2 antibody
    • Voltage-dependent T-type calcium channel subunit alpha-1H antibody
    • Voltage-gated calcium channel subunit alpha Cav3.2 antibody
    see all

References

This product has been referenced in:

  • Chen J  et al. Roles and mechanisms of TRPC3 and the PLC?/PKC/CPI-17 signaling pathway in regulating parturition. Mol Med Rep 17:898-910 (2018). Read more (PubMed: 29115500) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

- Have you used correct filters? The ab150079 absorption maxima is 650 nm while for FITC (ab98692) it is 495.
- Were the cells permeabilized?
- Is the sequence of insert protein is interpreted. It may well be that the insert sequence is missing which ab128251 binds.
The antibodies ab128251 and a84815 are raised against different part of the immunogen.
- Have you tried optimizing the results by incubating the antibody overnight or by increasing the concentration?

In order for checking the quality of ab150079 you may put a small drop of ab on slide and then seeing it under microscope. If it emits red colour then conjugate is fine and the problem may be with other steps. If it's not then problem is with secondary antibody.

It will be very helpful if you could provide the protcol followed step by step.

Please note that flow cytometry is not a tested application for ab128251 so the Abreview guarantee will not be valid. However if this antibody fails in ELISA, WB and ICC/IF then guarantee will apply as normal product.

Thank you very much for your cooperation. I will look forward to hearing from you soon.

Read More

Answer

Thank you for contacting Abcam.

The exact epitopes sequence for ab128251 is proprietary information and I do not have access to this information. A synthetic peptide corresponding to the N terminal domain of Human CACNA1H, (Uniprot: O95180) was used as the immunogen.

If there is anything else I can help you with, please let me know.

Read More

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