Overview

  • Product name

    Anti-CACNA1H antibody [N55/10]
    See all CACNA1H primary antibodies
  • Description

    Mouse monoclonal [N55/10] to CACNA1H
  • Host species

    Mouse
  • Specificity

    No cross-reactivity against Cav1.3 and Cav3.1
  • Tested applications

    Suitable for: IHC-P, IP, WB, ICC/IF, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Fusion protein, corresponding to amino acids 1019-1293 (II-III loop) of Human CACNA1H (accession number O95180)

  • Positive control

    • HEK cell lysate expressing Cav3.2
  • General notes

    The clone number has been updated from S55-10 to N55/10, both clone numbers name the same antibody clone.

Properties

Applications

Our Abpromise guarantee covers the use of ab84815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IP
WB
ICC/IF
IHC-Fr
  • Application notes
    ICC/IF: Use at a concentration of 0.1 - 1 µg/ml. (Perox), 1 - 10 µg/ml (IF).
    IP: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 10 µg/ml. Detects a band of approximately 260 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1H gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes.
    • Tissue specificity

      Expressed in kidney, liver, heart, brain. Isoform 2 seems to be testis-specific.
    • Involvement in disease

      Defects in CACNA1H are a cause of susceptibility to idiopathic generalized epilepsy type 6 (IGE6) [MIM:611942]. IGE is characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain. IGE6 is a polygenic and multifactorial disease.
    • Sequence similarities

      Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1H subfamily.
    • Domain

      Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
    • Post-translational
      modifications

      In response to raising of intracellular calcium, the T-type channels are activated by CaM-kinase II.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Alpha1 3.2 antibody
      • Alpha13.2 antibody
      • CAC1H_HUMAN antibody
      • CACNA 1H antibody
      • CACNA1 H antibody
      • CACNA1 HB antibody
      • Cacna1h antibody
      • CACNA1HB antibody
      • Calcium channel alpha13.2 subunit antibody
      • Calcium channel voltage dependent T type alpha 1H subunit antibody
      • Calcium channel, voltage-dependent, T type, alpha 1Hb subunit antibody
      • Cav 3.2 antibody
      • Cav T.2 antibody
      • Cav3.2 antibody
      • CavT.2 antibody
      • EIG 6 antibody
      • EIG6 antibody
      • Low voltage activated calcium channel alpha 13.2 subunit antibody
      • Low voltage activated calcium channel alpha1 3.2 subunit antibody
      • Low-voltage-activated calcium channel alpha1 3.2 subunit antibody
      • MNCb 1209 antibody
      • T type Cav3.2 antibody
      • Voltage dependent t type calcium channel alpha 1H subunit antibody
      • Voltage gated calcium channel alpha subunit Cav 3.2 antibody
      • Voltage gated calcium channel alpha subunit Cav T.2 antibody
      • Voltage gated calcium channel alpha subunit Cav3.2 antibody
      • Voltage gated calcium channel alpha subunit CavT.2 antibody
      • Voltage-dependent T-type calcium channel subunit alpha-1H antibody
      • Voltage-gated calcium channel subunit alpha Cav3.2 antibody
      see all

    Images

    • Immunohistochemical analysis of frozen Human brain tissue sections staining CACNA1H. Samples were fixed with 10% Formalin solution and incubated with primary antibody at 1:1000 dilution for 1 hour at room temperature. A biotinylated goat anti-mouse was used as the secondary antibody. Mayer Hematoxylin was used as a nuclear stain. 

    • ab84815 staining CACCNA1H in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).

    References

    This product has been referenced in:

    • Kürtüncü M  et al. CACNA1H antibodies associated with headache with neurological deficits and cerebrospinal fluid lymphocytosis (HaNDL). Cephalalgia : (2012). WB ; Human . Read more (PubMed: 23111027) »
    • Ohkubo T & Yamazaki J T-type voltage-activated calcium channel Cav3.1, but not Cav3.2, is involved in the inhibition of proliferation and apoptosis in MCF-7 human breast cancer cells. Int J Oncol 41:267-75 (2012). Read more (PubMed: 22469755) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

    I have read the details you have kindly provided and have following further questions for better understanding of the problem;

    - Have you used correct filters? The ab150079 absorption maxima is 650 nm while for FITC (ab98692) it is 495.
    - Were the cells permeabilized?
    - Is the sequence of insert protein is interpreted. It may well be that the insert sequence is missing which ab128251 binds.
    The antibodies ab128251 and a84815 are raised against different part of the immunogen.
    - Have you tried optimizing the results by incubating the antibody overnight or by increasing the concentration?

    In order for checking the quality of ab150079 you may put a small drop of ab on slide and then seeing it under microscope. If it emits red colour then conjugate is fine and the problem may be with other steps. If it's not then problem is with secondary antibody.

    It will be very helpful if you could provide the protcol followed step by step.

    Please note that flow cytometry is not a tested application for ab128251 so the Abreview guarantee will not be valid. However if this antibody fails in ELISA, WB and ICC/IF then guarantee will apply as normal product.

    Thank you very much for your cooperation. I will look forward to hearing from you soon.

    Read More

    Answer

    Thank you for contacting us and your interest in our products.

    Both the goat polyclonal anti-mouse IgG (ab97239 and ab98692) should be compatible with the mouse anti-CACNA1H antibody (ab84815) for the experiment you hope to perform. The antibody ab98692 is provided pre-adsorbed. This can be very useful when using multi-color imaging and IHC experiments when several primary antibodies and their corresponding secondary antibodies are used simultaneously. It also reduces the risk of cross-reactivity between the secondary antibody and endogenous immunoglobulins present on cell and tissue samples. It can therefore help to reduce non-specific staining.

    I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

    Read More

    Answer

    I am sorry to hear that you have been experiencing problems using these two products in the application that you wish.
    In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.
    As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.
    All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.
    Please could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.
    Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

    Read More

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