Key features and details
- Mouse monoclonal [N55/10] to CACNA1H
- Suitable for: IHC-P, IP, WB, ICC/IF, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-CACNA1H antibody [N55/10]
See all CACNA1H primary antibodies
DescriptionMouse monoclonal [N55/10] to CACNA1H
SpecificityNo cross-reactivity against Cav1.3 and Cav3.1
Tested applicationsSuitable for: IHC-P, IP, WB, ICC/IF, IHC-Frmore details
Species reactivityReacts with: Human
Fusion protein, corresponding to amino acids 1019-1293 (II-III loop) of Human CACNA1H (accession number O95180)
- HEK cell lysate expressing Cav3.2
The clone number has been updated from S55-10 to N55/10, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab84815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 10 µg/ml. Detects a band of approximately 260 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionVoltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1H gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes.
Tissue specificityExpressed in kidney, liver, heart, brain. Isoform 2 seems to be testis-specific.
Involvement in diseaseDefects in CACNA1H are a cause of susceptibility to idiopathic generalized epilepsy type 6 (IGE6) [MIM:611942]. IGE is characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain. IGE6 is a polygenic and multifactorial disease.
Sequence similaritiesBelongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1H subfamily.
DomainEach of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
modificationsIn response to raising of intracellular calcium, the T-type channels are activated by CaM-kinase II.
- Information by UniProt
- Alpha1 3.2 antibody
- Alpha13.2 antibody
- CAC1H_HUMAN antibody
Immunohistochemical analysis of frozen Human brain tissue sections staining CACNA1H. Samples were fixed with 10% Formalin solution and incubated with primary antibody at 1:1000 dilution for 1 hour at room temperature. A biotinylated goat anti-mouse was used as the secondary antibody. Mayer Hematoxylin was used as a nuclear stain.
ab84815 staining CACCNA1H in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab84815 has been referenced in 2 publications.
- Kürtüncü M et al. CACNA1H antibodies associated with headache with neurological deficits and cerebrospinal fluid lymphocytosis (HaNDL). Cephalalgia : (2012). WB ; Human . PubMed: 23111027
- Ohkubo T & Yamazaki J T-type voltage-activated calcium channel Cav3.1, but not Cav3.2, is involved in the inhibition of proliferation and apoptosis in MCF-7 human breast cancer cells. Int J Oncol 41:267-75 (2012). PubMed: 22469755