• Product name
    Anti-CACNA1S antibody [1A]
    See all CACNA1S primary antibodies
  • Description
    Mouse monoclonal [1A] to CACNA1S
  • Host species
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IP, WB, IHC-P, Inhibition Assay, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Guinea pig, Human
  • Immunogen

    Full length native protein (purified) corresponding to Rabbit CACNA1S. Purified from rabbit muscle T-tubule dihydropyridine receptor.

  • Positive control
    • rat skeletal muscle lysate


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    Voltage-sensitive calcium channels mediate the entry of calcium into many types of excitable cells and thus play a key role in neurotransmitter release and excitation-contraction (E-C) coupling. The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type calcium channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. The DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling. The purified DHP receptor isolated from triads is composed of at least four subunits. The alpha-1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated sodium channel. The alpha-2 subunit is a large glycoprotein associated with the DHP receptor which was first described in skeletal muscle and is also found in high concentrations in other excitable tissues such as cardiac muscle and brain and in low concentrations in most other tissues studied. The other two subunits that co-purify with the DHP receptor are termed beta and gamma.
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab2862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 21693436
IHC-Fr 1/200. PubMed: 21474431
IP Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 200 kDa.
IHC-P 1/20.
Inhibition Assay Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Function
    Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1S gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1S subunit play an important role in excitation-contraction coupling in skeletal muscle.
  • Tissue specificity
    Skeletal muscle specific.
  • Involvement in disease
    Defects in CACNA1S are the cause of periodic paralysis hypokalemic type 1 (HOKPP1) [MIM:170400]; also designated HYPOPP. HOKPP1 is an autosomal dominant disorder manifested by episodic flaccid generalized muscle weakness associated with falls of serum potassium levels.
    Defects in CACNA1S are the cause of malignant hyperthermia susceptibility type 5 (MHS5) [MIM:601887]; an autosomal dominant disorder that is potentially lethal in susceptible individuals on exposure to commonly used inhalational anesthetics and depolarizing muscle relaxants.
    Defects in CACNA1S are the cause of susceptibility to thyrotoxic periodic paralysis type 1 (TTPP1) [MIM:188580]. A sporadic muscular disorder characterized by episodic weakness and hypokalemia during a thyrotoxic state. It is clinically similar to hereditary hypokalemic periodic paralysis, except for the fact that hyperthyroidism is an absolute requirement for disease manifestation. The disease presents with recurrent episodes of acute muscular weakness of the four extremities that vary in severity from paresis to complete paralysis. Attacks are triggered by ingestion of a high carbohydrate load or strenuous physical activity followed by a period of rest. Thyrotoxic periodic paralysis can occur in association with any cause of hyperthyroidism, but is most commonly associated with Graves disease.
  • Sequence similarities
    Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1S subfamily.
  • Domain
    Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
    The loop between repeats II and III interacts with the ryanodine receptor, and is therefore important for calcium release from the endoplasmic reticulum necessary for muscle contraction.
  • Post-translational
    Phosphorylation by PKA activates the calcium channel.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha-1 polypeptide antibody
    • CAC1S_HUMAN antibody
    • CACH1 antibody
    • Cach1b antibody
    • CACN1 antibody
    • CACNA1S antibody
    • CACNL1A3 antibody
    • Calcium channel antibody
    • Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal antibody
    • Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal muscle antibody
    • Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal muscle, hypokalemic periodic paralysis antibody
    • Calcium channel, skeletal muscle dihydropyridine sensitive , alpha 1 subunit antibody
    • Calcium channel, voltage dependent, L type, alpha 1S subunit antibody
    • Calcium channel, voltage-dependent, L type, alpha 1S subunit, b antibody
    • Cav1.1 antibody
    • CCHL1A3 antibody
    • Dihydropyridine receptor antibody
    • Dihydropyridine sensitive L type calcium channel alpha 1 subunit antibody
    • fmd antibody
    • HOKPP antibody
    • HypoPP antibody
    • isoform 3 antibody
    • L type antibody
    • Malignant hyperthermia susceptibility 5 antibody
    • mdg antibody
    • MHS5 antibody
    • ROB1 antibody
    • sj antibody
    • skeletal muscle antibody
    • TTPP1 antibody
    • Voltage gated calcium channel subunit alpha Cav1.1 antibody
    • Voltage-dependent L-type calcium channel subunit alpha-1S antibody
    • Voltage-gated calcium channel subunit alpha Cav1.1 antibody
    see all


  • Anti-CACNA1S antibody [1A] (ab2862) at 1/2000 dilution + Mouse skeletal muscle microsomes lysate at 10 µg

    HRP-conjugated goat anti-mouse IgG, F(ab')2 polyclonal at 1/30000 dilution

    Performed under reducing conditions.

    Observed band size: 180 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa (possible non-specific binding), 36 kDa (possible non-specific binding)

    Exposure time: 2 minutes

    Blocked with 5% milk for 30 minutes at room temperature. Incubated with the primary antibody diluted in 5% skim milk in TBST for 8 hours at 4°C.

    See Abreview

  • Lanes 1-4 are ab2862 used to detect CACNA1S in lysate prepared from rabbit hearts. Each lane represents a separate heart.
    Frozen tissue chunks were pulverized under liquid nitrogen, and homogenized in 10 vol of buffer (0.25 M sucrose, 1 mM EDTA [pH 7.4]). This and all the following procedures took place in the presence of the protease inhibitor cocktail at 4°C or on ice. The homogenate was centrifuged at 3,500 rpm for 10 min to pellet nuclei and debris. The supernatant was centrifuged at 30,000 rpm for 1 hr to pellet the membranes. The membrane pellet was washed with a solution (Tris-HCl 20 mM [pH 7.4], EDTA 1 mM) and referred to as post-nuclear membrane fraction. The post-nuclear membrane fraction was rehomogenized in a Triton-containing lysis buffer (Tris-HCl 20 mM [pH 7.5], NaCl 0.2 M, EDTA 1 mM, Triton X-100 1%) using Dounce grinder. The mixture was centrifuged at 17,000 rpm for 1 hr, and the supernatant (Triton extract) was used for immunoblotting.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human skeletal muscle tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then incubated with ab2862 (1:20) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.


This product has been referenced in:
See all 10 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Western blot
Mouse Tissue lysate - other (Skeletal muscle microsomes)
Gel Running Conditions
Reduced Denaturing (4–15%)
Loading amount
10 µg
Skeletal muscle microsomes
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jun 15 2015

Immunocytochemistry/ Immunofluorescence
Mouse Cell (C2C12 myotube)
Yes - 0.1% triton/PBS
C2C12 myotube
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jun 15 2015


Thank you for your enquiry. Regarding the two antibodies, ab2862 and ab2825, I was able to find the specific antibody concentrations for you. The concentration of ab2825 (PMCA ATPase) is 7.7 mg/ml and the concentration of ab2862 (Dihydropyridine Receptor Alpha-1) is 3.8 mg/ml. I would recommend a range for immunoprecipitation assays, 10-500 ug of sample to 0.5-5 ug of antibody. Based on resources I have available, I would recommend starting in the mid-range. I hope this information helps. Please let me know if I can be of further assistance.

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Thank you for your enquiry. This antibody has been shown to work by Western blot; detecting a ~200 kDa protein corresponding to the DHP receptor in rat skeletal muscle extracts. You shouldn't have too much of a problem with your IP and western experiments given that IgG migrates at ~50KDa and therefore wont interfere with your targeting of DHPR alpha 1. I would recommend the use of low leaching protein A agarose and localising your blot for all products >100KDa by cutting your membrane with a scalpel. I thought that I would mention that DHPR alpha 1 was detected in skeletal muscle in the following publication. http://www.jbc.org/cgi/reprint/277/52/50535 Zheng Z, Wang ZM, Delbono O. Insulin-like growth factor-1 increases skeletal muscle dihydropyridine receptor alpha 1S transcriptional activity by acting on the cAMP-response element-binding protein element of the promoter region. J Biol Chem. 2002 Dec 27;277(52):50535-42. Epub 2002 Oct 28. Erratum in: J Biol Chem. 2003 May 2;278(18):16453. PMID: 12407098 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Thank you for your enquiry. I am sorry that ab2862 is not working for you. Since you are using the antibody in a tested application for a species that the antibody has been shown to cross-react with, this should work. Without knowing the exact problem you are having, I can only make general suggestions assuming you are seeing no staining. Have you tried blocking with 10% goat serum in place of BSA? You may wish to try this for 30 minutes at room temperature. In addition, you may wish to try incubating with the primary antibody overnight at 4 degrees. If you could give me further details of the protocol you are using by submitting the form below, I would be happy to look into this further and try to help you to get this antibody to work. https://www.abcam.com/index.html?section=ihc&pageconfig=technical&intAbID=2862&mode=questionaire I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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This antibody has not been epitope mapped. The following are references in regards to IHC: Neuron, 5: 339-351, 1990. J. Cell Biol., 123(5): 1161-1174, 1993. Am. J. Physiol., 268(37): F251-F257, 1995. If you have any more questions, please contact us again.

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