Validated using a knockout cell line
Recombinant
RabMAb

Anti-CACNA2D2 antibody [EPR12343] (ab173293)

Overview

  • Product name
    Anti-CACNA2D2 antibody [EPR12343]
    See all CACNA2D2 primary antibodies
  • Description
    Rabbit monoclonal [EPR12343] to CACNA2D2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human CACNA2D2. The exact sequence is proprietary.
    Database link: Q9NY47

  • Positive control
    • Fetal lung, fetal brain and Human cerebellum lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab173293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 130 kDa.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) and possibly T-type (CACNA1G). Overexpression induces apoptosis.
    • Tissue specificity
      Predominantly present in cerebellar cortex. Present in various lung tumor cell lines, while it is absent in normal lung (at protein level). Highly expressed in heart, lung, testis, pancreas and skeletal muscle. Also expressed in kidney, liver, placenta and brain.
    • Sequence similarities
      Belongs to the calcium channel subunit alpha-2/delta family.
      Contains 1 cache domain.
      Contains 1 VWFA domain.
    • Domain
      The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch.
    • Post-translational
      modifications
      May be proteolytically processed into subunits alpha-2-2 and delta-2 that are disulfide-linked. It is however unclear whether such cleavage really takes place in vivo and has a functional role.
    • Cellular localization
      Membrane. Colocalizes with CACNA1A in lipid raft fractions.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha 2 delta calcium channel subunit antibody
      • CA2D2_HUMAN antibody
      • CACNA2D antibody
      • Cacna2d2 antibody
      • Calcium channel, voltage-dependent, alpha 2/delta subunit 2 antibody
      • Calcium channel, voltage-dependent, alpha 2/delta subunit 2 gene 26 antibody
      • Calcium voltage gated channel auxiliary subunit alpha2delta 2 antibody
      • Gene 26 antibody
      • KIAA0558 antibody
      • LUAC11.1 antibody
      • Voltage-dependent calcium channel subunit alpha-2/delta-2 antibody
      • Voltage-dependent calcium channel subunit delta-2 antibody
      • Voltage-gated calcium channel subunit alpha-2/delta-2 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2:
      CACNA2D2 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Human brain tissue lysate (20 µg)
      Lanes 1 - 4:
      Merged signal (red and green). Green - ab173293 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab173293 was shown to specifically react with CACNA2D2 when CACNA2D2 knockout samples were used. Wild-type and CACNA2D2 knockout samples were subjected to SDS-PAGE. ab173293 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-CACNA2D2 antibody [EPR12343] (ab173293) at 1/1000 dilution

      Lane 1 : Fetal lung lysate
      Lane 2 : Fetal brain lysate
      Lane 3 : Human cerebellum lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 130 kDa

    • Western blot analysis on immunoprecipitation pellet from fetal brain lysate using ab173293.

    References

    ab173293 has not yet been referenced specifically in any publications.

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