Overview

  • Product name

    Anti-CACNB1/CAB1 antibody [N7/18]
    See all CACNB1/CAB1 primary antibodies
  • Description

    Mouse monoclonal [N7/18] to CACNB1/CAB1
  • Host species

    Mouse
  • Specificity

    No cross reactivity against CACNB4.
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow
  • Immunogen

    Synthetic peptide corresponding to Rat CACNB1/CAB1 aa 19-34.
    Sequence:

    PMEVFDPSPQGKYSKR


    Database link: NP_059042

  • Positive control

    • Rat brain lysate.
  • General notes

    The clone number has been updated from S7-18 to N7/18, both clone numbers name the same antibody clone.

     

    Previously labelled as CACNB1.

Properties

Applications

Our Abpromise guarantee covers the use of ab85020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 66 kDa.
ICC/IF Use a concentration of 1 - 10 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
IHC-P Use a concentration of 0.1 - 1 µg/ml.

Target

  • Function

    The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.
  • Tissue specificity

    Isoform 1 and isoform 3 are expressed in brain, heart, spleen, central nervous system and neuroblastoma cells. Isoform 2 is expressed in skeletal muscle.
  • Sequence similarities

    Belongs to the calcium channel beta subunit family.
    Contains 1 SH3 domain.
  • Cellular localization

    Cell membrane > sarcolemma.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAB1 antibody
    • CACB1_HUMAN antibody
    • CACNB1 antibody
    • CACNLB1 antibody
    • Calcium channel L type beta 1 polypeptide antibody
    • Calcium channel voltage dependent beta 1 subunit antibody
    • Calcium channel voltage-dependent subunit beta 1 antibody
    • CCHLB1 antibody
    • Dihydropyridine sensitive L type calcium channel beta 1 subunit antibody
    • MGC41896 antibody
    • Voltage dependent L type calcium channel beta 1 subunit antibody
    • Voltage-dependent L-type calcium channel subunit beta-1 antibody
    see all

Images

  • ab85020 staining CACNB1/CAB1 in human hippocampus tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.

  • Anti-CACNB1/CAB1 antibody [N7/18] (ab85020) at 1/1000 dilution + Rat brain membrane lysate at 15 µg with 1.5% BSA for 30 minutes at RT

    Secondary
    Sheep Anti-Mouse IgG: HRP for 1 hour at RT

    Predicted band size: 66 kDa

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - other (Hippocampal synaptosome preparation)
Gel Running Conditions
Reduced Denaturing (Bis Tris 4-12%)
Loading amount
5 µg
Specification
Hippocampal synaptosome preparation
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 18 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up