Key features and details
- Mouse monoclonal [N7/18] to CACNB1/CAB1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Product nameAnti-CACNB1/CAB1 antibody [N7/18]
See all CACNB1/CAB1 primary antibodies
DescriptionMouse monoclonal [N7/18] to CACNB1/CAB1
SpecificityNo cross reactivity against CACNB4.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow
- Rat brain lysate.
The clone number has been updated from S7-18 to N7/18, both clone numbers name the same antibody clone.
Previously labelled as CACNB1.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab85020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 66 kDa.|
|IHC-P||Use a concentration of 0.1 - 1 µg/ml.|
FunctionThe beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.
Tissue specificityIsoform 1 and isoform 3 are expressed in brain, heart, spleen, central nervous system and neuroblastoma cells. Isoform 2 is expressed in skeletal muscle.
Sequence similaritiesBelongs to the calcium channel beta subunit family.
Contains 1 SH3 domain.
Cellular localizationCell membrane > sarcolemma.
- Information by UniProt
- CAB1 antibody
- CACB1_HUMAN antibody
- CACNB1 antibody
ab85020 staining CACNB1/CAB1 in human hippocampus tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.
Anti-CACNB1/CAB1 antibody [N7/18] (ab85020) at 1/1000 dilution + Rat brain membrane lysate at 15 µg with 1.5% BSA for 30 minutes at RT
Sheep Anti-Mouse IgG: HRP for 1 hour at RT
Predicted band size: 66 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab85020 has been referenced in 1 publication.
- Lim JA et al. Defects in calcium homeostasis and mitochondria can be reversed in Pompe disease. Autophagy 11:385-402 (2015). PubMed: 25758767