Key features and details
- Mouse monoclonal [N8B/1] to CACNB2
- Suitable for: IHC-P, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-CACNB2 antibody [N8B/1]
See all CACNB2 primary antibodies
DescriptionMouse monoclonal [N8B/1] to CACNB2
SpecificityNo cross reactivity against CAVB1, CAVB3 and CAVB4
Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cytmore details
Species reactivityReacts with: Mouse, Rat, Human
- IHC-P: Human heart tissue. Flow Cyt: SH-SY5Y cells. ICC/IF: HaCaT cells. IP: Mouse skeletal muscle lysate.
The clone number has been updated from S8b-1 to N8B/1, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab93606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Antigen retrieval is not essential but may optimise staining.|
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|
|Flow Cyt||Use 1µl for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionThe beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.
Tissue specificityExpressed in all tissues.
Involvement in diseaseDefects in CACNB2 are the cause of Brugada syndrome type 4 (BRS4) [MIM:611876]. BRS4 is a heart disease characterized by the association of Brugada syndrome with shortened QT intervals. Brugada syndrome is a tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset.
Sequence similaritiesBelongs to the calcium channel beta subunit family.
Contains 1 SH3 domain.
Cellular localizationCell membrane > sarcolemma.
- Information by UniProt
- AW060387 antibody
- Ca(V) beta 2 antibody
- CAB2 antibody
IHC image of ab93606 staining in Human Heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93606, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Overlay histogram showing SH-SY5Y cells stained with ab93606 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93606, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Cold 100% methanol-fixed HaCaT (human keratinocyte cell line) cells stained for CACNB2 (green) using ab93606 at 1/100 dilution in ICC/IF.
Secondary Antibody: FITC Goat Anti-Mouse at 1/50 dilution for 1 hour at room temperature.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab93606 has been referenced in 2 publications.
- Johnson EK et al. Identification of new dystroglycan complexes in skeletal muscle. PLoS One 8:e73224 (2013). Mouse . PubMed: 23951345
- Miranda-Laferte E et al. A Short Polybasic Segment between the Two Conserved Domains of the ß2a-Subunit Modulates the Rate of Inactivation of R-type Calcium Channel. J Biol Chem 287:32588-97 (2012). WB . PubMed: 22851179