Anti-CACNB2 antibody [N8B/1] (ab93606)

Reviews (1)Q&A (4)References (2)

Overview

  • Product name

    Anti-CACNB2 antibody [N8B/1]
    See all CACNB2 primary antibodies

  • Description

    Mouse monoclonal [N8B/1] to CACNB2

  • Host species

    Mouse

  • Specificity

    No cross reactivity against CAVB1, CAVB3 and CAVB4

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide:

    RSPKPSANSVTSPHSKE

    , corresponding to internal sequence amino acids 189 - 205 of Rat CACNB2 (NP_446303).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human heart tissue. Flow Cyt: SH-SY5Y cells. ICC/IF: HaCaT cells. IP: Mouse skeletal muscle lysate.

  • General notes

    The clone number has been updated from S8b-1 to N8B/1, both clone numbers name the same antibody clone.

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    N8B/1

  • Isotype

    IgG1

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 74 kDa).
IHC-P Use a concentration of 5 µg/ml. Antigen retrieval is not essential but may optimise staining.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
ICC/IF Use a concentration of 1 - 10 µg/ml.
Flow Cyt Use 1µl for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IP Use at an assay dependent concentration.

Target

  • Function

    The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.

  • Tissue specificity

    Expressed in all tissues.

  • Involvement in disease

    Defects in CACNB2 are the cause of Brugada syndrome type 4 (BRS4) [MIM:611876]. BRS4 is a heart disease characterized by the association of Brugada syndrome with shortened QT intervals. Brugada syndrome is a tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset.

  • Sequence similarities

    Belongs to the calcium channel beta subunit family.
    Contains 1 SH3 domain.

  • Cellular localization

    Cell membrane > sarcolemma.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AW060387 antibody
    • Ca(V) beta 2 antibody
    • CAB2 antibody
    • CACB2_HUMAN antibody
    • CACNB2 antibody
    • CACNLB2 antibody
    • Calcium channel voltage dependent subunit beta 2 antibody
    • Calcium channel voltage-dependent subunit beta 2 antibody
    • Calcium channel, voltage dependent, beta 2 subunit antibody
    • CAVB2 antibody
    • Cavbeta2 antibody
    • Cchb2 antibody
    • FLJ23743 antibody
    • Lambert Eaton Myasthenic syndrome antigen antibody
    • Lambert Eaton myasthenic syndrome antigen B antibody
    • Lambert-Eaton myasthenic syndrome antigen B antibody
    • MGC129334 antibody
    • MGC129335 antibody
    • Myasthenic (Lambert Eaton) syndrome antigen B antibody
    • Myasthenic syndrome antigen B antibody
    • MYSB antibody
    • Voltage dependent L type calcium channel subunit beta 2 antibody
    • Voltage-dependent L-type calcium channel subunit beta-2 antibody
    see all

Images

  • Immunoprecipitation - Anti-CACNB2 antibody [N8B/1] (ab93606)
    Immunoprecipitation - Anti-CACNB2 antibody [N8B/1] (ab93606)This image is courtesy of an anonymous Abreview
    CACNB2 immunoprecipitation from mouse skeletal muscle tissue using ab93606. 500 µg of cell lysate was incubated with undiluted primary antibody and matrix (Protein G) and incubated for 12 hours at 4°C.

    Lane 1: Mouse skeletal muscle lysate
    Lane 2: anti-CACNB2 IP

    Western blot detection: ab93606 at 1/500 dilution

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNB2 antibody [N8B/1] (ab93606)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNB2 antibody [N8B/1] (ab93606)
    IHC image of ab93606 staining in Human Heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93606, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Flow Cytometry - Anti-CACNB2 antibody [N8B/1] (ab93606)
    Flow Cytometry - Anti-CACNB2 antibody [N8B/1] (ab93606)
    Overlay histogram showing SH-SY5Y cells stained with ab93606 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93606, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Anti-CACNB2 antibody [N8B/1] (ab93606)
    Immunocytochemistry/ Immunofluorescence - Anti-CACNB2 antibody [N8B/1] (ab93606)

    Cold 100% methanol-fixed HaCaT (human keratinocyte cell line) cells stained for CACNB2 (green) using ab93606 at 1/100 dilution in ICC/IF.

    Secondary Antibody: FITC Goat Anti-Mouse at 1/50 dilution for 1 hour at room temperature.

References

This product has been referenced in:

  • Johnson EK  et al. Identification of new dystroglycan complexes in skeletal muscle. PLoS One 8:e73224 (2013). Mouse . Read more (PubMed: 23951345) »
  • Miranda-Laferte E  et al. A Short Polybasic Segment between the Two Conserved Domains of the ß2a-Subunit Modulates the Rate of Inactivation of R-type Calcium Channel. J Biol Chem 287:32588-97 (2012). WB . Read more (PubMed: 22851179) »
See all 2 Publications for this product

Publishing research using ab93606? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Immunoprecipitation abreview for Anti-CACNB2 antibody [S8b-1]

 Excellent
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (skeletal muscle)
Total protein in input
500 µg
Specification
skeletal muscle
Immuno-precipitation step
Protein G
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Abcam user community

Verified customer

Submitted Jan 09 2013

Question

I would like to test the Anti-CACNB2 antibody [S8b-1] (ab93606) for Immunoprecipitation on mouse muscle tissue.

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Answer

I am very pleased to hear you would like to test ab93606 in IP. This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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Question

Our immunoblot result of ab93606 and ab49131 are not conclusive and I am wondering are there blocking peptides available for us to try?    

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Answer

I am sorry to read that you are still having trouble obtaining a good blot for CACNB2. Are you testing the same samples as before, rat brain homogenates? We do not have a blocking peptide for either antibody. For ab93606, you were not obtaining any signal. What is the result of staining blots with ab49131?

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Question

Thank you very much for your time and help.

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Answer

Good luck! Please let us know how it goes. If you want to submit a review, please see details at the following webpage: www.abcam.com/abreviews  

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Question

No signal in western blots of rat brain homogenates and cell lysates

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Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***** for ab49131. To check the status of the order please contact our Customer Service team and reference this number.  Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I am very interested to know how this works with rat samples as this species, though predicted to react with this antibody, has not been tested before. I wish you the best of luck with your research.  

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