Overview

  • Product name

    Anti-CACNB4 antibody [N10/7]
    See all CACNB4 primary antibodies
  • Description

    Mouse monoclonal [N10/7] to CACNB4
  • Host species

    Mouse
  • Specificity

    No cross reactivity against rat Cav beta 1b, Cav beta 2a and Cav beta 3 in transfected cells.
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Fr, IP, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 458-474 of Rat CACNB4

  • Positive control

    • Rat brain lysate.
  • General notes

    The clone number has been updated from S10-7 to N10/7, both clone numbers name the same antibody clone.

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    N10/7
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 58 kDa.
ICC/IF Use a concentration of 1 - 10 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P Use a concentration of 0.1 - 1 µg/ml.

Target

  • Function

    The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.
  • Tissue specificity

    Expressed predominantly in the cerebellum and kidney.
  • Involvement in disease

    Genetic variations in CACNB4 are the cause of susceptibility to idiopathic generalized epilepsy type 9 (IGE9) [MIM:607682]. IGE9 is characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain.
    Genetic variations in CACNB4 are the cause of susceptibility to juvenile myoclonic epilepsy type 6 (EJM6) [MIM:607682]. EJM6 is a subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue.
  • Sequence similarities

    Belongs to the calcium channel beta subunit family.
    Contains 1 SH3 domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAB4 antibody
    • CACB4_HUMAN antibody
    • Cacnb4 antibody
    • CACNLB4 antibody
    • Calcium channel voltage dependent beta 4 subunit antibody
    • Calcium channel voltage dependent subunit beta 4 antibody
    • Calcium channel voltage-dependent subunit beta 4 antibody
    • Dihydropyridine sensitive L type calcium channel beta 4 subunit antibody
    • EA5 antibody
    • EIG9 antibody
    • EJM antibody
    • EJM4 antibody
    • EJM6 antibody
    • Voltage-dependent L-type calcium channel subunit beta-4 antibody
    see all

Images

  • All lanes : Anti-CACNB4 antibody [N10/7] (ab85788) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Lysates prepared from CHO-T cells transfected with CACNB4 gene

    Predicted band size: 58 kDa

  • Overlay histogram showing SH-SH5Y cells stained with ab85788 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85788, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • ab85788 staining CACNB4 in human hippocapmpus tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.

  • Anti-CACNB4 antibody [N10/7] (ab85788) at 1/1000 dilution
    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 58 kDa



    All lanes: Mouse Hippocampal synaptosome tissue lysate (5µg)

    Secondary Antibody: An HRP-conjugated Goat Monoclonal 1/10000 dilution

    Blocking Step: 5% Milk for 1 hour at 25°C

    See Abreview

References

This product has been referenced in:

  • Scott MB & Kammermeier PJ CaV2 channel subtype expression in rat sympathetic neurons is selectively regulated by a2d subunits. Channels (Austin) 11:555-573 (2017). Read more (PubMed: 28837380) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - other (Hippocampal synaptosome preparation)
Loading amount
5 µg
Specification
Hippocampal synaptosome preparation
Gel Running Conditions
Reduced Denaturing (Bis Tris 4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Feb 17 2010

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