Key features and details
- Rabbit polyclonal to CacyBP
- Suitable for: ICC, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-CacyBP antibody
See all CacyBP primary antibodies
DescriptionRabbit polyclonal to CacyBP
Tested applicationsSuitable for: ICC, WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- Rat liver, brain and spleen tissue lysates; SMMC, COLO320, SW620 A549 and 293T cell lysates. Rat brain tissue, Human liver cancer tissue
This product was previously labelled as SIAH Interacting Protein
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab190950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use a concentration of 0.5 - 1 µg/ml.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 26 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).
Sequence similaritiesContains 1 CS domain.
Contains 1 SGS domain.
modificationsPhosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations.
Cellular localizationNucleus. Cytoplasm. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.
- Information by UniProt
- CacyBP antibody
- Calcyclin binding protein antibody
- Calcyclin-binding protein antibody
All lanes : Anti-CacyBP antibody (ab190950) at 0.5 µg/ml
Lane 1 : Rat liver tissue lysate at 50 µg
Lane 2 : Rat brain tissue lysate at 50 µg
Lane 3 : Rat spleen tissue lysate at 50 µg
Lane 4 : SMMC cell lysate at 40 µg
Lane 5 : COLO320 cell lysate at 40 µg
Lane 6 : SW620 cell lysate at 40 µg
Lane 7 : 293T cell lysate at 40 µg
Predicted band size: 26 kDa
Observed band size: 26 kDa
Immunocytochemical analysis of A549 cells staining CacyBP with ab190950 at 1 µg/ml.
Immunonhistochemical analysis of paraffin-embedded human liver cancer tissue sections labelling CacyBP with ab190950 at dilution of 0.5μg/mL. Antigen retrieval was performed by heat.
Immunonhistochemical analysis of paraffin-embedded rat brain tissue sections labelling CacyBP with ab190950 at dilution of 0.5μg/mL. Antigen retrieval was performed by heat.
ab190950 has not yet been referenced specifically in any publications.