Overview

  • Product name

  • Description

    Rabbit polyclonal to CacyBP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human CacyBP aa 160-175 (internal sequence).
    Sequence:

    NTRWDYLTQVEKECKE


    Database link: Q9HB71

  • Positive control

    • Rat liver, brain and spleen tissue lysates; SMMC, COLO320, SW620 A549 and 293T cell lysates. Rat brain tissue, Human liver cancer tissue
  • General notes

     This product was previously labelled as SIAH Interacting Protein

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 26 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).
  • Sequence similarities

    Contains 1 CS domain.
    Contains 1 SGS domain.
  • Post-translational
    modifications

    Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.
  • Information by UniProt
  • Database links

  • Alternative names

    • CacyBP antibody
    • Calcyclin binding protein antibody
    • Calcyclin-binding protein antibody
    • CYBP_HUMAN antibody
    • GIG 5 antibody
    • GIG5 antibody
    • Growth inhibiting gene 5 antibody
    • Growth inhibiting gene 5 protein antibody
    • hCacyBP antibody
    • MGC87971 antibody
    • PNAS 107 antibody
    • PNAS107 antibody
    • RP1 102G20.6 antibody
    • S100A6 binding protein antibody
    • S100A6-binding protein antibody
    • S100A6BP antibody
    • Siah-interacting protein antibody
    • SIP antibody
    see all

Images

  • All lanes : Anti-CacyBP antibody (ab190950) at 0.5 µg/ml

    Lane 1 : Rat liver tissue lysate at 50 µg
    Lane 2 : Rat brain tissue lysate at 50 µg
    Lane 3 : Rat spleen tissue lysate at 50 µg
    Lane 4 : SMMC cell lysate at 40 µg
    Lane 5 : COLO320 cell lysate at 40 µg
    Lane 6 : SW620 cell lysate at 40 µg
    Lane 7 : 293T cell lysate at 40 µg

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

  • Immunocytochemical analysis of A549 cells staining CacyBP with ab190950 at 1 µg/ml.

  • Immunonhistochemical analysis of paraffin-embedded human liver cancer tissue sections labelling CacyBP with ab190950 at dilution of 0.5μg/mL. Antigen retrieval was performed by heat.

  • Immunonhistochemical analysis of paraffin-embedded rat brain tissue sections labelling CacyBP with ab190950 at dilution of 0.5μg/mL. Antigen retrieval was performed by heat.

References

ab190950 has not yet been referenced specifically in any publications.

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