Product nameAnti-CacyBP antibody
See all CacyBP primary antibodies
DescriptionRabbit polyclonal to CacyBP
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human CacyBP aa 150-200. The exact sequence is proprietary. (NP_055227.1).
Database link: Q9HB71
- WB: HeLa whole cell lysate.
This product was previously labelled as SIAH Interacting Protein
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225557 was affinity purified using an epitope specific to CacyBP immobilized on solid support.
Our Abpromise guarantee covers the use of ab225557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 26 kDa.|
FunctionMay be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).
Sequence similaritiesContains 1 CS domain.
Contains 1 SGS domain.
modificationsPhosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations.
Cellular localizationNucleus. Cytoplasm. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.
- Information by UniProt
- CacyBP antibody
- Calcyclin binding protein antibody
- Calcyclin-binding protein antibody
All lanes : Anti-CacyBP antibody (ab225557) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 3 minutes
ab225557 has not yet been referenced specifically in any publications.