Overview

  • Product name

  • Description

    Rabbit polyclonal to CacyBP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human CacyBP aa 150-200. The exact sequence is proprietary. (NP_055227.1).
    Database link: Q9HB71

  • Positive control

    • WB: HeLa whole cell lysate.
  • General notes

     This product was previously labelled as SIAH Interacting Protein

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab225557 was affinity purified using an epitope specific to CacyBP immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab225557 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 26 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).
    • Sequence similarities

      Contains 1 CS domain.
      Contains 1 SGS domain.
    • Post-translational
      modifications

      Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations.
    • Cellular localization

      Nucleus. Cytoplasm. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.
    • Information by UniProt
    • Database links

    • Alternative names

      • CacyBP antibody
      • Calcyclin binding protein antibody
      • Calcyclin-binding protein antibody
      • CYBP_HUMAN antibody
      • GIG 5 antibody
      • GIG5 antibody
      • Growth inhibiting gene 5 antibody
      • Growth inhibiting gene 5 protein antibody
      • hCacyBP antibody
      • MGC87971 antibody
      • PNAS 107 antibody
      • PNAS107 antibody
      • RP1 102G20.6 antibody
      • S100A6 binding protein antibody
      • S100A6-binding protein antibody
      • S100A6BP antibody
      • Siah-interacting protein antibody
      • SIP antibody
      see all

    Images

    • All lanes : Anti-CacyBP antibody (ab225557) at 0.04 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
      Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
      Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 26 kDa


      Exposure time: 3 minutes

    References

    ab225557 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab225557.
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