Recombinant
RabMAb

Recombinant Anti-CacyBP antibody [EPR12374] (ab171972)

Overview

  • Product name

    Anti-CacyBP antibody [EPR12374]
    See all CacyBP primary antibodies
  • Description

    Rabbit monoclonal [EPR12374] to CacyBP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    within Human CacyBP aa 200 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9HB71

  • Positive control

    • Human kidney, gastric carcinoma and gliocytoma tissues; MCF7, HeLa, 293T and PC3 cell lysates; MCF7 and 293T cells.
  • General notes

     This product was previously labelled as SIAH Interacting Protein

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12374
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).
  • Sequence similarities

    Contains 1 CS domain.
    Contains 1 SGS domain.
  • Post-translational
    modifications

    Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.
  • Information by UniProt
  • Database links

  • Alternative names

    • CacyBP antibody
    • Calcyclin binding protein antibody
    • Calcyclin-binding protein antibody
    • CYBP_HUMAN antibody
    • GIG 5 antibody
    • GIG5 antibody
    • Growth inhibiting gene 5 antibody
    • Growth inhibiting gene 5 protein antibody
    • hCacyBP antibody
    • MGC87971 antibody
    • PNAS 107 antibody
    • PNAS107 antibody
    • RP1 102G20.6 antibody
    • S100A6 binding protein antibody
    • S100A6-binding protein antibody
    • S100A6BP antibody
    • Siah-interacting protein antibody
    • SIP antibody
    see all

Images

  • All lanes : Anti-CacyBP antibody [EPR12374] (ab171972) at 1/10000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : PC3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 26 kDa

  • Immunohistochemical analysis of paraffin-embedded Human Kidney tissue labeling CacyBP with ab171972 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling CacyBP with ab171972 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling CacyBP with ab171972 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunofluorescent analysis of MCF7 cells labeling CacyBP with ab171972 at 1/100 dilution.  DAPI nuclear staining (blue).

  • Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) labeling CacyBP with ab171972 at 1/10 dilution or 1X PBS (lane 2) negative control.  HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

  • Flow cytometric analysis of permeabilized 293T cells labeling CacyBP with ab171972 at 1/10 dilution (red) or  Rabbit IgG (negative) (green).

References

ab171972 has not yet been referenced specifically in any publications.

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