Key features and details
- Rabbit polyclonal to CAD/BM1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CAD/BM1 antibody
See all CAD/BM1 primary antibodies
DescriptionRabbit polyclonal to CAD/BM1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to Human CAD/BM1 aa 1650-1700.
Database link: NP_004332.2
- HeLa, 293T and NIH3T3 whole cell lysates.
This product was previously labelled as CAD
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 243 kDa.|
RelevanceCarbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD.
Cellular localizationCytoplasmic and Nuclear
- Aspartate carbamoyltransferase antibody
- Aspartate transcarbamylase antibody
- CAD protein antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling CAD/BM1 with ab99312 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-CAD/BM1 antibody (ab99312) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysates at 50 µg
Lane 2 : HeLa whole cell lysates at 15 µg
Lane 3 : HeLa whole cell lysates at 5 µg
Lane 4 : 293T whole cell lysates at 50 µg
Lane 5 : NIH3T3 whole cell lysates at 50 µg
Predicted band size: 243 kDa
Exposure time: 3 seconds
ab99312 has been referenced in 3 publications.
- Cheng ML et al. Metabolic Reprogramming of Host Cells in Response to Enteroviral Infection. Cells 9:N/A (2020). PubMed: 32085644
- Pesini A et al. Brain pyrimidine nucleotide synthesis and Alzheimer disease. Aging (Albany NY) 11:8433-8462 (2019). PubMed: 31560653
- Roux C et al. Endogenous glutamine decrease is associated with pancreatic cancer progression. Oncotarget 8:95361-95376 (2017). PubMed: 29221133