• Product name

  • Description

    Rabbit polyclonal to CAD/BM1
  • Host species

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human CAD/BM1 aa 1650-1700.
    Database link: NP_004332.2

  • Positive control

    • HeLa, 293T and NIH3T3 whole cell lysates.
  • General notes

     This product was previously labelled as CAD




Our Abpromise guarantee covers the use of ab99312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 243 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance

      Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD.
    • Cellular localization

      Cytoplasmic and Nuclear
    • Database links

    • Alternative names

      • Aspartate carbamoyltransferase antibody
      • Aspartate transcarbamylase antibody
      • CAD protein antibody
      • CAD trifunctional protein antibody
      • Carbamoyl phosphate synthetase 2 antibody
      • Carbamoyl-phosphate synthetase 2 aspartate transcarbamylase and dihydroorotase antibody
      • CPSase ATCase DHOase antibody
      • Dihydroorotase antibody
      • Glutamine dependent carbamoyl phosphate synthase antibody
      • Multifunctional protein CAD antibody
      see all


    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling CAD/BM1 with ab99312 at 1/200 (1µg/ml). Detection: DAB.

    • All lanes : Anti-CAD/BM1 antibody (ab99312) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysates at 50 µg
      Lane 2 : HeLa whole cell lysates at 15 µg
      Lane 3 : HeLa whole cell lysates at 5 µg
      Lane 4 : 293T whole cell lysates at 50 µg
      Lane 5 : NIH3T3 whole cell lysates at 50 µg

      Predicted band size: 243 kDa

      Exposure time: 3 seconds


    This product has been referenced in:

    See 1 Publication for this product

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