Product nameAnti-CAD/BM1 antibody
See all CAD/BM1 primary antibodies
DescriptionRabbit polyclonal to CAD/BM1
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human CAD/BM1 aa 1850-2000.
Database link: NP_004332.2
- HeLa, 293T and NIH3T3 whole cell lysates.
This product was previously labelled as CAD
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab99313 was affinity purified using an epitope specific to CAD/BM1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab99313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 243 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceCarbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD.
Cellular localizationCytoplasmic and Nuclear
- Aspartate carbamoyltransferase antibody
- Aspartate transcarbamylase antibody
- CAD protein antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CAD/BM1 with ab99313 at 1/200 (1µg/ml). Detection: DAB.
All lanes : Anti-CAD/BM1 antibody (ab99313) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 243 kDa
Exposure time: 3 seconds
Detection of CAD/BM1 by Western Blot of Immunprecipitate.
ab99313 at 0.1µg/ml staining CAD/BM1 in HeLa whole cell lysate immunoprecipitated using ab99313 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab99313 has not yet been referenced specifically in any publications.