Anti-CAD antibody (ab99313)
Key features and details
- Rabbit polyclonal to CAD
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CAD antibody
See all CAD primary antibodies -
Description
Rabbit polyclonal to CAD -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human CAD aa 1850-2000.
Database link: NP_004332.2 -
Positive control
- WB: K-562, Jurkat, HeLa, 293T and NIH3T3 whole cell lysates. IP: Jurkat cell lysate. IHC-P: Human ovarian carcinoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab99313 was affinity purified using an epitope specific to CAD immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99313 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 243 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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IHC-P
1/200. |
WB
1/2000 - 1/10000. Predicted molecular weight: 243 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Relevance
Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 790 Human
- Entrez Gene: 69719 Mouse
- Omim: 114010 Human
- SwissProt: P27708 Human
- SwissProt: B2RQC6 Mouse
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Alternative names
- Aspartate carbamoyltransferase antibody
- Aspartate transcarbamylase antibody
- CAD protein antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CAD with ab99313 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-CAD antibody (ab99313) at 0.04 µg/ml
Lane 1 : HEK-293T whole cell lysate
Lane 2 : K562 whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 243 kDa
Exposure time: 10 seconds -
CAD Antibody | Bethyl Laboratories, Inc.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab99313 has not yet been referenced specifically in any publications.