Anti-CAD/BM1 antibody [EP711Y] (ab40765)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CAD/BM1 antibody [EP711Y]
See all CAD/BM1 primary antibodies -
Description
Rabbit monoclonal [EP711Y] to CAD/BM1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CAD/BM1 aa 2150-2250 (C terminal). The exact sequence is proprietary.
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Positive control
- 293 cell lysate; human breast carcinoma
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as CAD
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP711Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab40765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| WB | 1/1000 - 1/5000. Detects a band of approximately 250 kDa (predicted molecular weight: 250 kDa). | |
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 790 Human
- Omim: 114010 Human
- SwissProt: P27708 Human
- Unigene: 377010 Human
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Alternative names
- Aspartate carbamoyltransferase antibody
- Aspartate transcarbamylase antibody
- CAD protein antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAD/BM1 antibody [EP711Y] (ab40765)](https://www.abcam.com/ps/products/40/ab40765/Images/ab40765-53890-anti-cad-antibody-ep711y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAD/BM1 antibody [EP711Y] (ab40765)Immunohistochemical analysis of paraffin-embedded human breast carcinoma
using ab40765 at 1/250 dilution.Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Datasheets and documents
References
ab40765 has not yet been referenced specifically in any publications.
