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  1. Link

    cadbm1-antibody-ep711y-ab40765.pdf

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Cell Biology Apoptosis Nucleus DNAses
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RabMAb

Anti-CAD/BM1 antibody [EP711Y] (ab40765)

  • Datasheet
Submit a review Submit a question References (1)

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Western blot - Anti-CAD/BM1 antibody [EP711Y] (ab40765)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAD/BM1 antibody [EP711Y] (ab40765)

Key features and details

  • Rabbit monoclonal [EP711Y] to CAD/BM1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CAD/BM1 antibody [EP711Y]
    See all CAD/BM1 primary antibodies
  • Description

    Rabbit monoclonal [EP711Y] to CAD/BM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CAD/BM1 aa 2150-2250 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HEK-293 cell lysate IHC-P: Human breast carcinoma
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 99% Tissue culture supernatant, 0.1% BSA
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP711Y
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • DNAses
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Apoptosis
    • Nuclear
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • DNAses
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HEK-293 whole cell lysate (ab7902)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab40765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Detects a band of approximately 250 kDa (predicted molecular weight: 250 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/5000. Detects a band of approximately 250 kDa (predicted molecular weight: 250 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

    • Entrez Gene: 790 Human
    • Omim: 114010 Human
    • SwissProt: P27708 Human
    • Alternative names

      • Aspartate carbamoyltransferase antibody
      • Aspartate transcarbamylase antibody
      • CAD protein antibody
      • CAD trifunctional protein antibody
      • Carbamoyl phosphate synthetase 2 antibody
      • Carbamoyl-phosphate synthetase 2 aspartate transcarbamylase and dihydroorotase antibody
      • CPSase ATCase DHOase antibody
      • Dihydroorotase antibody
      • Glutamine dependent carbamoyl phosphate synthase antibody
      • Multifunctional protein CAD antibody
      see all

    Images

    • Western blot - Anti-CAD/BM1 antibody [EP711Y] (ab40765)
      Western blot - Anti-CAD/BM1 antibody [EP711Y] (ab40765)
      Anti-CAD/BM1 antibody [EP711Y] (ab40765) at 1/2000 dilution (Unpurified) + HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 250 kDa
      Observed band size: 245 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAD/BM1 antibody [EP711Y] (ab40765)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAD/BM1 antibody [EP711Y] (ab40765)

      Immunohistochemical analysis of paraffin-embedded human breast carcinoma
      using ab40765 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab40765? Please let us know so that we can cite the reference in this datasheet.

    ab40765 has been referenced in 1 publication.

    • Ridder DA  et al. Key Enzymes in Pyrimidine Synthesis, CAD and CPS1, Predict Prognosis in Hepatocellular Carcinoma. Cancers (Basel) 13:N/A (2021). PubMed: 33670206

    Customer reviews and Q&As

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