Anti-CAD/BM1 antibody [EP711Y] (ab40765)
![Western blot - Anti-CAD/BM1 antibody [EP711Y] (ab40765)](https://www.abcam.com/ps/products/40/ab40765/Images/ab40765-415223-anti-cad-bm1-antibody-ep711y-westernblot-hek-293-human.jpg "Western blot - Anti-CAD/BM1 antibody [EP711Y] (ab40765)")
Key features and details
- Rabbit monoclonal [EP711Y] to CAD/BM1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CAD/BM1 antibody [EP711Y]
See all CAD/BM1 primary antibodies -
Description
Rabbit monoclonal [EP711Y] to CAD/BM1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CAD/BM1 aa 2150-2250 (C terminal). The exact sequence is proprietary.
-
Positive control
- WB: HEK-293 cell lysate IHC-P: Human breast carcinoma
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 99% Tissue culture supernatant, 0.1% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP711Y -
Isotype
IgG -
Research areas
Associated products
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 250 kDa (predicted molecular weight: 250 kDa).
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 250 kDa (predicted molecular weight: 250 kDa). |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Relevance
Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 790 Human
- Omim: 114010 Human
- SwissProt: P27708 Human
-
Alternative names
- Aspartate carbamoyltransferase antibody
- Aspartate transcarbamylase antibody
- CAD protein antibody
see all
Images
-
Western blot - Anti-CAD/BM1 antibody [EP711Y] (ab40765)Anti-CAD/BM1 antibody [EP711Y] (ab40765) at 1/2000 dilution (Unpurified) + HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 250 kDa
Observed band size: 245 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAD/BM1 antibody [EP711Y] (ab40765)](https://www.abcam.com/ps/products/40/ab40765/Images/ab40765-53890-anti-cad-antibody-ep711y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAD/BM1 antibody [EP711Y] (ab40765)Immunohistochemical analysis of paraffin-embedded human breast carcinoma
using ab40765 at 1/250 dilution.Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (1)
ab40765 has been referenced in 1 publication.
- Ridder DA et al. Key Enzymes in Pyrimidine Synthesis, CAD and CPS1, Predict Prognosis in Hepatocellular Carcinoma. Cancers (Basel) 13:N/A (2021). PubMed: 33670206
