Overview

  • Product name

    Anti-CAD/BM1 (phospho T456) antibody
    See all CAD/BM1 primary antibodies
  • Description

    Rabbit polyclonal to CAD/BM1 (phospho T456)
  • Host species

    Rabbit
  • Specificity

    ab194534 detects endogenous levels of CAD/BM1 only when phosphorylated at threonine 456.

  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human CAD/BM1 (phospho T456) conjugated to keyhole limpet haemocyanin. Peptide sequence around phosphorylation site of threonine 456 (P-I-T(p)-P-H) derived from Human CAD/BM1.
    Database link: P27708

  • Positive control

    • Human colon carcinoma tissue.
  • General notes

     This product was previously labelled as CAD

Properties

Applications

Our Abpromise guarantee covers the use of ab194534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.

Target

  • Relevance

    Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD.
  • Cellular localization

    Cytoplasmic and Nuclear
  • Database links

  • Alternative names

    • Aspartate carbamoyltransferase antibody
    • Aspartate transcarbamylase antibody
    • CAD protein antibody
    • CAD trifunctional protein antibody
    • Carbamoyl phosphate synthetase 2 antibody
    • Carbamoyl-phosphate synthetase 2 aspartate transcarbamylase and dihydroorotase antibody
    • CPSase ATCase DHOase antibody
    • Dihydroorotase antibody
    • Glutamine dependent carbamoyl phosphate synthase antibody
    • Multifunctional protein CAD antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling CAD/BM1 (phospho T456) with ab194534 at 1/50 dilution. The image on the right has been pre-incubated with the blocking peptide.

References

ab194534 has not yet been referenced specifically in any publications.

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