Key features and details
- Rabbit polyclonal to CAD/BM1 (phospho T456)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CAD/BM1 (phospho T456) antibody
See all CAD/BM1 primary antibodies
DescriptionRabbit polyclonal to CAD/BM1 (phospho T456)
ab194534 detects endogenous levels of CAD/BM1 only when phosphorylated at threonine 456.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human CAD/BM1 (phospho T456) conjugated to keyhole limpet haemocyanin. Peptide sequence around phosphorylation site of threonine 456 (P-I-T(p)-P-H) derived from Human CAD/BM1.
Database link: P27708
- Human colon carcinoma tissue.
This product was previously labelled as CAD
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
PBS (without Mg2+and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab194534 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab194534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
RelevanceCarbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD.
Cellular localizationCytoplasmic and Nuclear
- Aspartate carbamoyltransferase antibody
- Aspartate transcarbamylase antibody
- CAD protein antibody
ab194534 has not yet been referenced specifically in any publications.