Recombinant
RabMAb

Recombinant Anti-cadherin 10 antibody [EPR5115] (ab134137)

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Overview

  • Product name

    Anti-cadherin 10 antibody [EPR5115]
    See all cadherin 10 primary antibodies

  • Description

    Rabbit monoclonal [EPR5115] to cadherin 10

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human cadherin 10 aa 750-850. The exact sequence is proprietary.
    (Peptide available as ab185240)

  • Positive control

    • Human fetal brain, fetal lung and U-87 MG; permeabilized U-87 MG cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab134137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 88 kDa.Can be blocked with cadherin 10 peptide (ab185240).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    • Function

      Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

    • Tissue specificity

      Predominantly expressed in brain. Also found in adult and fetal kidney. Very low levels detected in prostate and fetal lung.

    • Sequence similarities

      Contains 5 cadherin domains.

    • Domain

      Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • CAD10_HUMAN antibody
      • Cadherin 10 (T2 cadherin) antibody
      • Cadherin 10 type 2 (T2 cadherin) antibody
      • Cadherin 10 type 2 antibody
      • Cadherin-10 antibody
      • Cadherin10 antibody
      • CDH10 antibody
      • T2 cadherin antibody
      • T2-cadherin antibody
      see all

    Images

    • Western blot - Anti-cadherin 10 antibody [EPR5115] (ab134137)
      Western blot - Anti-cadherin 10 antibody [EPR5115] (ab134137)
      All lanes : Anti-cadherin 10 antibody [EPR5115] (ab134137) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : U-87 MG lysate
      Lane 3 : Human fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 88 kDa

    • Flow Cytometry - Anti-cadherin 10 antibody [EPR5115] (ab134137)
      Flow Cytometry - Anti-cadherin 10 antibody [EPR5115] (ab134137)
      Flow cytometric analysis of permeabilised U-87 MG cells labelling cadherin 10 with ab134137 at 1/10 dilution (red) or rabbit IgG negative (green).

    References

    ab134137 has not yet been referenced specifically in any publications.

    Publishing research using ab134137? Please let us know so that we can cite the reference in this datasheet.

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