Product nameAnti-cadherin 10 antibody [EPR5115]
See all cadherin 10 primary antibodies
DescriptionRabbit monoclonal [EPR5115] to cadherin 10
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human cadherin 10 aa 750-850. The exact sequence is proprietary.
(Peptide available as
- Human fetal brain, fetal lung and U-87 MG; permeabilized U-87 MG cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab134137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 88 kDa.Can be blocked with cadherin 10 peptide (ab185240).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
Tissue specificityPredominantly expressed in brain. Also found in adult and fetal kidney. Very low levels detected in prostate and fetal lung.
Sequence similaritiesContains 5 cadherin domains.
DomainThree calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
Cellular localizationCell membrane.
- Information by UniProt
- CAD10_HUMAN antibody
- Cadherin 10 (T2 cadherin) antibody
- Cadherin 10 type 2 (T2 cadherin) antibody
All lanes : Anti-cadherin 10 antibody [EPR5115] (ab134137) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : U-87 MG lysate
Lane 3 : Human fetal lung lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 88 kDa
Flow cytometric analysis of permeabilised U-87 MG cells labelling cadherin 10 with ab134137 at 1/10 dilution (red) or rabbit IgG negative (green).
ab134137 has not yet been referenced specifically in any publications.