Recombinant Anti-cadherin 10 antibody [EPR5115] (ab134137)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-cadherin 10 antibody [EPR5115]
See all cadherin 10 primary antibodies -
Description
Rabbit monoclonal [EPR5115] to cadherin 10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human cadherin 10 aa 750-850. The exact sequence is proprietary.
(Peptide available asab185240) -
Positive control
- Human fetal brain, fetal lung and U-87 MG; permeabilized U-87 MG cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5115 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab134137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 88 kDa.Can be blocked with cadherin 10 peptide (ab185240). | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. -
Tissue specificity
Predominantly expressed in brain. Also found in adult and fetal kidney. Very low levels detected in prostate and fetal lung. -
Sequence similarities
Contains 5 cadherin domains. -
Domain
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1008 Human
- Entrez Gene: 320873 Mouse
- Entrez Gene: 29181 Rat
- Omim: 604555 Human
- SwissProt: Q9Y6N8 Human
- SwissProt: P70408 Mouse
- Unigene: 92489 Human
- Unigene: 117794 Mouse
see all -
Alternative names
- CAD10_HUMAN antibody
- Cadherin 10 (T2 cadherin) antibody
- Cadherin 10 type 2 (T2 cadherin) antibody
see all
Images
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![Western blot - Anti-cadherin 10 antibody [EPR5115] (ab134137)](https://www.abcam.com/ps/products/134/ab134137/Images/ab134137-148383-anti-cadherin-10-antibody-epr5115-western-blot.jpg)
Western blot - Anti-cadherin 10 antibody [EPR5115] (ab134137)All lanes : Anti-cadherin 10 antibody [EPR5115] (ab134137) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : U-87 MG lysate
Lane 3 : Human fetal lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 88 kDa -
![Flow Cytometry - Anti-cadherin 10 antibody [EPR5115] (ab134137)](https://www.abcam.com/ps/products/134/ab134137/Images/ab134137-148382-anti-cadherin-10-antibody-epr5115-flow-cytometry.jpg)
Flow Cytometry - Anti-cadherin 10 antibody [EPR5115] (ab134137)Flow cytometric analysis of permeabilised U-87 MG cells labelling cadherin 10 with ab134137 at 1/10 dilution (red) or rabbit IgG negative (green).
Datasheets and documents
References
ab134137 has not yet been referenced specifically in any publications.
