RecombinantRabMAb

Recombinant Anti-cadherin 10 antibody [EPR5115] (ab134137)

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Western blot - Anti-cadherin 10 antibody [EPR5115] (ab134137)
  • Flow Cytometry - Anti-cadherin 10 antibody [EPR5115] (ab134137)
  • Anti-cadherin 10 antibody [EPR5115] (ab134137)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5115] to cadherin 10
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-cadherin 10 antibody [EPR5115]
    See all cadherin 10 primary antibodies

  • Description

    Rabbit monoclonal [EPR5115] to cadherin 10

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human cadherin 10 aa 750-850. The exact sequence is proprietary.
    (Peptide available as ab185240)

  • Positive control

    • Human fetal brain, fetal lung and U-87 MG; permeabilized U-87 MG cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 88 kDa.Can be blocked with cadherin 10 peptide (ab185240).
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 88 kDa.Can be blocked with cadherin 10 peptide (ab185240).
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    • Function

      Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

    • Tissue specificity

      Predominantly expressed in brain. Also found in adult and fetal kidney. Very low levels detected in prostate and fetal lung.

    • Sequence similarities

      Contains 5 cadherin domains.

    • Domain

      Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • CAD10_HUMAN antibody
      • Cadherin 10 (T2 cadherin) antibody
      • Cadherin 10 type 2 (T2 cadherin) antibody
      • Cadherin 10 type 2 antibody
      • Cadherin-10 antibody
      • Cadherin10 antibody
      • CDH10 antibody
      • T2 cadherin antibody
      • T2-cadherin antibody
      see all

    Images

    • Western blot - Anti-cadherin 10 antibody [EPR5115] (ab134137)
      Western blot - Anti-cadherin 10 antibody [EPR5115] (ab134137)
      All lanes : Anti-cadherin 10 antibody [EPR5115] (ab134137) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : U-87 MG lysate
      Lane 3 : Human fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 88 kDa

    • Flow Cytometry - Anti-cadherin 10 antibody [EPR5115] (ab134137)
      Flow Cytometry - Anti-cadherin 10 antibody [EPR5115] (ab134137)
      Flow cytometric analysis of permeabilised U-87 MG cells labelling cadherin 10 with ab134137 at 1/10 dilution (red) or rabbit IgG negative (green).
    • Anti-cadherin 10 antibody [EPR5115] (ab134137)
      Anti-cadherin 10 antibody [EPR5115] (ab134137)

    References (1)

    Publishing research using ab134137? Please let us know so that we can cite the reference in this datasheet.

    ab134137 has been referenced in 1 publication.

    • Witte H  et al. A Sam68-dependent alternative splicing program shapes postsynaptic protein complexes. Eur J Neurosci 49:1436-1453 (2019). PubMed: 30589479

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