Overview

  • Product name

    Anti-cadherin 10 antibody [EPR5115]
    See all cadherin 10 primary antibodies
  • Description

    Rabbit monoclonal [EPR5115] to cadherin 10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human cadherin 10 aa 750-850. The exact sequence is proprietary.
    (Peptide available as ab185240)

  • Positive control

    • Human fetal brain, fetal lung and U-87 MG; permeabilized U-87 MG cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 88 kDa.Can be blocked with cadherin 10 peptide (ab185240).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function

      Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
    • Tissue specificity

      Predominantly expressed in brain. Also found in adult and fetal kidney. Very low levels detected in prostate and fetal lung.
    • Sequence similarities

      Contains 5 cadherin domains.
    • Domain

      Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CAD10_HUMAN antibody
      • Cadherin 10 (T2 cadherin) antibody
      • Cadherin 10 type 2 (T2 cadherin) antibody
      • Cadherin 10 type 2 antibody
      • Cadherin-10 antibody
      • Cadherin10 antibody
      • CDH10 antibody
      • T2 cadherin antibody
      • T2-cadherin antibody
      see all

    Images

    • All lanes : Anti-cadherin 10 antibody [EPR5115] (ab134137) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : U-87 MG lysate
      Lane 3 : Human fetal lung lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 88 kDa

    • Flow cytometric analysis of permeabilised U-87 MG cells labelling cadherin 10 with ab134137 at 1/10 dilution (red) or rabbit IgG negative (green).

    References

    ab134137 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134137.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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