Recombinant Anti-Cadherin 16 antibody [EPR13090] (ab183745)
![Western blot - Anti-Cadherin 16 antibody [EPR13090] (ab183745)](https://www.abcam.com/ps/products/183/ab183745/Images/ab183745-215814-anti-cadherin-16-antibody-epr13090-western-blot.jpg "Western blot - Anti-Cadherin 16 antibody [EPR13090] (ab183745)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13090] to Cadherin 16
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Cadherin 16 antibody [EPR13090]
See all Cadherin 16 primary antibodies -
Description
Rabbit monoclonal [EPR13090] to Cadherin 16 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human Cadherin 16 aa 50-200. The exact sequence is proprietary.
Database link: O75309 -
Positive control
- Human fetal kidney lysate; Human chromophobe renal cell carcinoma and mouse kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13090 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab183745 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 90 kDa). | |
| IHC-P | 1/4000 - 1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| IP | 1/40 - 1/60. |
Target
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. -
Tissue specificity
Kidney specific. -
Sequence similarities
Contains 6 cadherin domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1014 Human
- Entrez Gene: 12556 Mouse
- Omim: 603118 Human
- SwissProt: O75309 Human
- SwissProt: O88338 Mouse
- Unigene: 513660 Human
- Unigene: 19423 Mouse
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Alternative names
- CAD16_HUMAN antibody
- Cadherin 16 antibody
- Cadherin 16 KSP cadherin antibody
see all
Images
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Western blot - Anti-Cadherin 16 antibody [EPR13090] (ab183745)Anti-Cadherin 16 antibody [EPR13090] (ab183745) at 1/10000 dilution + Human fetal kidney lysate at 10 µg
Secondary
Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 90 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] (ab183745)](https://www.abcam.com/ps/products/183/ab183745/Images/ab183745-215813-anti-cadherin-16-antibody-epr13090-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] (ab183745)Immunohistochemical analysis of paraffin-embedded Human chromophobe renal cell carcinoma tissue labeling Cadherin 16 with ab183745 at 1/4000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] (ab183745)](https://www.abcam.com/ps/products/183/ab183745/Images/ab183745-215811-anti-cadherin-16-antibody-epr13090-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] (ab183745)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Cadherin 16 with ab183745 at 1/4000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Cadherin 16 antibody [EPR13090] (ab183745)](https://www.abcam.com/ps/products/183/ab183745/Images/ab183745-215810-anti-cadherin-16-antibody-epr13090-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Cadherin 16 antibody [EPR13090] (ab183745)Western blot analysis of Cadherin 16 in Human fetal kidney lysate immunoprecipitated using ab183745 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
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![Anti-Cadherin 16 antibody [EPR13090] (ab183745)](https://www.abcam.com/ps/products/183/ab183745/Images/ab183745-5-benefits-of-recombinant-antibodies.png)
Anti-Cadherin 16 antibody [EPR13090] (ab183745)
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab183745 has not yet been referenced specifically in any publications.
