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Signal Transduction Cytoskeleton / ECM Cell Adhesion Cadherins
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RecombinantRabMAb

Recombinant Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (ab214092)

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  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (ab214092)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (ab214092)
  • Immunoprecipitation - Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (ab214092)
  • Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (ab214092)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13090] to Cadherin 16 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free
    See all Cadherin 16 primary antibodies
  • Description

    Rabbit monoclonal [EPR13090] to Cadherin 16 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human Cadherin 16 aa 50-200. The exact sequence is proprietary.
    Database link: O75309

  • Positive control

    • Human fetal kidney lysate; Human chromophobe renal cell carcinoma and mouse kidney tissues.
  • General notes

    Ab214092 is the carrier-free version of ab183745. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab214092 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13090
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins

Associated products

  • Alternative Versions

    • Anti-Cadherin 16 antibody [EPR13090] (ab183745)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

Our Abpromise guarantee covers the use of ab214092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 130 kDa (predicted molecular weight: 90 kDa).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
  • Tissue specificity

    Kidney specific.
  • Sequence similarities

    Contains 6 cadherin domains.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession O75309 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1014 Human
    • Entrez Gene: 12556 Mouse
    • Omim: 603118 Human
    • SwissProt: O75309 Human
    • SwissProt: O88338 Mouse
    • Unigene: 513660 Human
    • Unigene: 19423 Mouse
    • Alternative names

      • CAD16_HUMAN antibody
      • Cadherin 16 antibody
      • Cadherin 16 KSP cadherin antibody
      • Cadherin kidney antibody
      • Cadherin-16 antibody
      • CDH 16 antibody
      • CDH16 antibody
      • Kidney cadherin antibody
      • Kidney specific cadherin antibody
      • Kidney-specific cadherin antibody
      • Ksp cadherin antibody
      • Ksp-cadherin antibody
      • PRO1340 antibody
      • UNQ695 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (ab214092)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (ab214092)

      Immunohistochemical analysis of paraffin-embedded Human chromophobe renal cell carcinoma tissue labeling Cadherin 16 with ab183745 at 1/4000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183745).

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (ab214092)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (ab214092)

      Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Cadherin 16 with ab183745 at 1/4000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183745).

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (ab214092)
      Immunoprecipitation - Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (ab214092)

      Western blot analysis of Cadherin 16 in Human fetal kidney lysate immunoprecipitated using ab183745 at 1/50 dilution.

      Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183745).

    • Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (ab214092)
      Anti-Cadherin 16 antibody [EPR13090] - BSA and Azide free (ab214092)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab214092? Please let us know so that we can cite the reference in this datasheet.

    ab214092 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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