Overview

  • Product name
    Anti-Cadherin 22 antibody
  • Description
    Rabbit polyclonal to Cadherin 22
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cadherin 22 aa 410-440 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9UJ99

  • Positive control
    • HepG2, 293 and K562 cell line lysates. Human brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab171616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 89 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. PB-cadherins may have a role in the morphological organization of pituitary gland and brain tissues.
  • Sequence similarities
    Contains 5 cadherin domains.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C20orf25 antibody
    • CAD22_HUMAN antibody
    • Cadherin 22, type 2 antibody
    • Cadherin-22 antibody
    • Cadherin-like 22 antibody
    • Cdh22 antibody
    • dJ998H6.1 antibody
    • MGC39564 antibody
    • Ortholog of rat PB-cadherin antibody
    • PB-cadherin antibody
    • Pituitary and brain cadherin antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed and paraffin embedded Human brain tissue labeling Cadherin 22  with ab171616 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • All lanes : Anti-Cadherin 22 antibody (ab171616) at 1/100 dilution

    Lane 1 : HepG2 cell line lysate
    Lane 2 : 293 cell line lysate
    Lane 3 : K562 cell line lysate

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 89 kDa

References

This product has been referenced in:
  • Martín-Sánchez E  et al. CDH22 hypermethylation is an independent prognostic biomarker in breast cancer. Clin Epigenetics 9:7 (2017). Read more (PubMed: 28149335) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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