Anti-Cadherin 8 antibody (ab97268)
Key features and details
- Rabbit polyclonal to Cadherin 8
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cadherin 8 antibody
See all Cadherin 8 primary antibodies -
Description
Rabbit polyclonal to Cadherin 8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following whole cell lysates: SHSY5Y; WERI; U87MG; SKNBE.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97268 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
Chicken
Cow
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 88 kDa).
|
|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 88 kDa). |
|
ICC/IF
Use a concentration of 5 µg/ml. |
Target
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. -
Tissue specificity
Mainly expressed in brain. Found in certain nerve cell lines, such as retinoblasts, glioma cells and neuroblasts. -
Sequence similarities
Contains 5 cadherin domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1006 Human
- Entrez Gene: 12564 Mouse
- Entrez Gene: 84408 Rat
- Omim: 603008 Human
- SwissProt: P55286 Human
- SwissProt: P97291 Mouse
- SwissProt: O54800 Rat
- Unigene: 368322 Human
see all -
Alternative names
- CADH8_HUMAN antibody
- Cadherin 8, type 2 antibody
- Cadherin-8 antibody
see all
Images
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Western blot - Anti-Cadherin 8 antibody (ab97268)All lanes : Anti-Cadherin 8 antibody (ab97268) at 1 µg/ml
Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 2 : WERI (Human Retinoblastoma) Whole Cell Lysate
Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lane 4 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
Cadherin 8 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunocytochemistry/ Immunofluorescence - Anti-Cadherin 8 antibody (ab97268)ICC/IF image of ab97268 stained U87MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97268, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (1)
ab97268 has been referenced in 1 publication.
- Mills F et al. Cadherins mediate cocaine-induced synaptic plasticity and behavioral conditioning. Nat Neurosci 20:540-549 (2017). PubMed: 28192395
