Recombinant Anti-Cadherin 9 antibody [EPR5113] (ab124803)

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Western blot - Anti-Cadherin 9 antibody [EPR5113] (ab124803)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5113] to Cadherin 9
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-Cadherin 9 antibody [EPR5113]
See all Cadherin 9 primary antibodies
Description

Rabbit monoclonal [EPR5113] to Cadherin 9
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Cadherin 9 aa 750 to the C-terminus (intracellular). The exact sequence is proprietary.
Positive control
- 293T, U87-MG, Human testis, NIH/3T3 and Caco 2 lysates; Permeabilized 293T cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 2.40 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5113
Isotype

IgG
Research areas

Alternative Versions
- Anti-Cadherin 9 antibody [EPR5113] - BSA and Azide free (ab213652)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Caco-2 whole cell lysate (ab3950)
- NIH/3T3 whole cell lysate (ab7179)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
Flow Cyt	Human
WB	Human
All applications	Rat

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).
Flow Cyt		1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).
Flow Cyt 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes

Is unsuitable for ICC,IHC-P or IP.

Function

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
Sequence similarities

Contains 5 cadherin domains.
Domain

Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
Cellular localization

Cell membrane.
Target information above from: UniProt accession Q9ULB4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1007 Human
- Entrez Gene: 12565 Mouse
- Entrez Gene: 29163 Rat
- Omim: 609974 Human
- SwissProt: Q9ULB4 Human
- SwissProt: P70407 Mouse
- Unigene: 272212 Human
- Unigene: 439758 Mouse
- Unigene: 222436 Rat
see all
Alternative names
- CADH9_HUMAN antibody
- Cadherin 9 type 2 antibody
- Cadherin-9 antibody
- Cadherin9 antibody
- CDH 9 antibody
- CDH9 antibody
- T1 cadherin antibody
see all

Western blot - Anti-Cadherin 9 antibody [EPR5113] (ab124803)

All lanes : Anti-Cadherin 9 antibody [EPR5113] (ab124803) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : Human testis lysate
Lane 4 : NIH/3T3 cell lysate
Lane 5 : Caco 2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 88 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Flow Cytometry - Anti-Cadherin 9 antibody [EPR5113] (ab124803)

Flow cytometric analysis of permeabilized 293T cells using ab124803 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
OI-RD Scanning - Anti-Cadherin 9 antibody [EPR5113] (ab124803)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Cadherin 9 antibody [EPR5113] (ab124803)

Western blot protocols
Flow cytometry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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Publishing research using ab124803? Please let us know so that we can cite the reference in this datasheet.

ab124803 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (ES Cells and Neural cells)

Permeabilization

Yes - 0.4%-1% Triton

Specification

ES Cells and Neural cells

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Formaldehyde

Maneeshi Prasad

Verified customer

Submitted Jul 21 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Domain

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (0)

Customer reviews and Q&As

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cadherin 9 antibody [EPR5113]

Dissociation constant (K_D)