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RecombinantRabMAb

Recombinant Anti-Cadherin 9 antibody [EPR5113] (ab124803)

  • Datasheet
  • SDS
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Western blot - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
  • Flow Cytometry - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
  • OI-RD Scanning - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
  • Anti-Cadherin 9 antibody [EPR5113] (ab124803)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5113] to Cadherin 9
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Cadherin 9 antibody [EPR5113]
    See all Cadherin 9 primary antibodies
  • Description

    Rabbit monoclonal [EPR5113] to Cadherin 9
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Cadherin 9 aa 750 to the C-terminus (intracellular). The exact sequence is proprietary.

  • Positive control

    • 293T, U87-MG, Human testis, NIH/3T3 and Caco 2 lysates; Permeabilized 293T cells
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.40 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5113
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cadherins
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction

Associated products

  • Alternative Versions

    • Anti-Cadherin 9 antibody [EPR5113] - BSA and Azide free (ab213652)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Caco-2 whole cell lysate (ab3950)
    • NIH/3T3 whole cell lysate (ab7179)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Human
All applications
Rat
Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
    • Sequence similarities

      Contains 5 cadherin domains.
    • Domain

      Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession Q9ULB4 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1007 Human
      • Entrez Gene: 12565 Mouse
      • Entrez Gene: 29163 Rat
      • Omim: 609974 Human
      • SwissProt: Q9ULB4 Human
      • SwissProt: P70407 Mouse
      • Unigene: 272212 Human
      • Unigene: 439758 Mouse
      • Unigene: 222436 Rat
      see all
    • Alternative names

      • CADH9_HUMAN antibody
      • Cadherin 9 type 2 antibody
      • Cadherin-9 antibody
      • Cadherin9 antibody
      • CDH 9 antibody
      • CDH9 antibody
      • T1 cadherin antibody
      see all

    Images

    • Western blot - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      Western blot - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      All lanes : Anti-Cadherin 9 antibody [EPR5113] (ab124803) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : Human testis lysate
      Lane 4 : NIH/3T3 cell lysate
      Lane 5 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 88 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?

    • Flow Cytometry - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      Flow Cytometry - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      Flow cytometric analysis of permeabilized 293T cells using ab124803 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
    • OI-RD Scanning - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      OI-RD Scanning - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      Anti-Cadherin 9 antibody [EPR5113] (ab124803)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab124803? Please let us know so that we can cite the reference in this datasheet.

    ab124803 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cadherin 9 antibody [EPR5113]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (ES Cells and Neural cells)
    Permeabilization
    Yes - 0.4%-1% Triton
    Specification
    ES Cells and Neural cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Maneeshi Prasad

    Verified customer

    Submitted Jul 21 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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