Recombinant Anti-Cadherin 9 antibody [EPR5113] (ab124803)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5113] to Cadherin 9
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Cadherin 9 antibody [EPR5113]
See all Cadherin 9 primary antibodies -
Description
Rabbit monoclonal [EPR5113] to Cadherin 9 -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanWB Human -
Immunogen
Synthetic peptide within Human Cadherin 9 aa 750 to the C-terminus (intracellular). The exact sequence is proprietary.
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Positive control
- 293T, U87-MG, Human testis, NIH/3T3 and Caco 2 lysates; Permeabilized 293T cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.40 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5113 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
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WB |
Human
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All applications |
Rat
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Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).
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Flow Cyt |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa). |
Flow Cyt
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. -
Sequence similarities
Contains 5 cadherin domains. -
Domain
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1007 Human
- Entrez Gene: 12565 Mouse
- Entrez Gene: 29163 Rat
- Omim: 609974 Human
- SwissProt: Q9ULB4 Human
- SwissProt: P70407 Mouse
- Unigene: 272212 Human
- Unigene: 439758 Mouse
see all -
Alternative names
- CADH9_HUMAN antibody
- Cadherin 9 type 2 antibody
- Cadherin-9 antibody
see all
Images
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All lanes : Anti-Cadherin 9 antibody [EPR5113] (ab124803) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : Human testis lysate
Lane 4 : NIH/3T3 cell lysate
Lane 5 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 88 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
Flow cytometric analysis of permeabilized 293T cells using ab124803 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab124803 has not yet been referenced specifically in any publications.