Recombinant
RabMAb

Recombinant Anti-Cadherin 9 antibody [EPR5113] (ab124803)

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Overview

  • Product name

    Anti-Cadherin 9 antibody [EPR5113]
    See all Cadherin 9 primary antibodies

  • Description

    Rabbit monoclonal [EPR5113] to Cadherin 9

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Cadherin 9 aa 750-850 (intracellular). The exact sequence is proprietary.

  • Positive control

    • 293T, U87-MG, Human testis, NIH/3T3 and Caco 2 lysates; Permeabilized 293T cells

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

    • Sequence similarities

      Contains 5 cadherin domains.

    • Domain

      Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • CADH9_HUMAN antibody
      • Cadherin 9 type 2 antibody
      • Cadherin-9 antibody
      • Cadherin9 antibody
      • CDH 9 antibody
      • CDH9 antibody
      • T1 cadherin antibody
      see all

    Images

    • Western blot - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      Western blot - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      All lanes : Anti-Cadherin 9 antibody [EPR5113] (ab124803) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : Human testis lysate
      Lane 4 : NIH/3T3 cell lysate
      Lane 5 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 88 kDa
      Observed band size: 110 kDa
      why is the actual band size different from the predicted?

    • Flow Cytometry - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      Flow Cytometry - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      Flow cytometric analysis of permeabilized 293T cells using ab124803 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
    • Other - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      Other - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab124803 has not yet been referenced specifically in any publications.

    Publishing research using ab124803? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cadherin 9 antibody [EPR5113]

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (ES Cells and Neural cells)
    Permeabilization
    Yes - 0.4%-1% Triton
    Specification
    ES Cells and Neural cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Maneeshi Prasad

    Verified customer

    Submitted Jul 21 2016

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