Product nameAnti-Cadherin 9 antibody [EPR5113]
See all Cadherin 9 primary antibodies
DescriptionRabbit monoclonal [EPR5113] to Cadherin 9
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Cadherin 9 aa 750-850 (intracellular). The exact sequence is proprietary.
- 293T, U87-MG, Human testis, NIH/3T3 and Caco 2 lysates; Permeabilized 293T cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.40 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
Sequence similaritiesContains 5 cadherin domains.
DomainThree calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
Cellular localizationCell membrane.
- Information by UniProt
- CADH9_HUMAN antibody
- Cadherin 9 type 2 antibody
- Cadherin-9 antibody
All lanes : Anti-Cadherin 9 antibody [EPR5113] (ab124803) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : Human testis lysate
Lane 4 : NIH/3T3 cell lysate
Lane 5 : Caco 2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 88 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Flow cytometric analysis of permeabilized 293T cells using ab124803 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124803 has not yet been referenced specifically in any publications.