Anti-Calbindin antibody (ab49899)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-Calbindin antibody
See all Calbindin primary antibodies -
Description
Rabbit polyclonal to Calbindin -
Host species
Rabbit -
Specificity
We recommend the use of RabMAb ab108404 for testing in IHC and ICC/IF for this target. -
Tested applications
Suitable for: IHC-Fr, IP, IHC-FoFr, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Hamster, Cow, Pig
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Calbindin.
Read Abcam's proprietary immunogen policy (Peptide available as ab60452.) -
Positive control
- This antibody gave a positive signal in the following tissues: Kidney (Mouse), Kidney (Human), Brain (human), Brain (Mouse) Brain (Rat)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab49899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | 1/200. | |
| IP | Use at an assay dependent concentration. | |
| IHC-FoFr | 1/300. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 30 kDa). |
Target
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Function
Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. -
Sequence similarities
Belongs to the calbindin family.
Contains 5 EF-hand domains. -
Domain
This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium. - Information by UniProt
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Database links
- Entrez Gene: 539930 Cow
- Entrez Gene: 793 Human
- Entrez Gene: 12307 Mouse
- Entrez Gene: 100153645 Pig
- Entrez Gene: 83839 Rat
- Omim: 114050 Human
- SwissProt: P04467 Cow
- SwissProt: P05937 Human
see all -
Alternative names
- avian-type antibody
- CAB27 antibody
- CALB 1 antibody
see all
Images
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Calbindin antibody (ab49899)This image is courtesy of an abreview submitted by Sophie Pezet, CNRS, Paris, FranceImmunohistochemistical detection of Calbindin using antibody ab49899 on PFA perfusion-fixed frozen rat brain sections (30um). Antigen retrieval step: None Primary Antibody diluted at 1/300 and incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary Antibody: Goat anti-rabbit Alexa Fluor® 488 (1/1000). ab49899 was used on sections of rat brain in free floating IHC; expected staining was observed on rat brain. The submitted images show staining obtained in the cerebral cortex (A,B). The images were taken using the objective X5 (A) and X40 (B) and show the neuronal profiles (arrows) and neuronal processes.
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Western blot - Anti-Calbindin antibody (ab49899)All lanes : ab49899 at 1 µg/ml
Lane 1 : Brain (human) tissue lysate
Lane 2 : Brain (Mouse) Tissue lysate
Lane 3 : Brain (Rat) Tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 37 kDa, 51 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Western blot - Anti-Calbindin antibody (ab49899)All lanes : Anti-Calbindin antibody (ab49899) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 150 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-Calbindin antibody (ab49899)Calbindin - Neuronal Marker was immunoprecipitated using 0.5mg Mouse kidney whole tissue extract, 5µg of Rabbit polyclonal to Calbindin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse kidney whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab49899.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 30kDa: Calbindin - Neuronal Marker.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Schludi MH et al. Spinal poly-GA inclusions in a C9orf72 mouse model trigger motor deficits and inflammation without neuron loss. Acta Neuropathol 134:241-254 (2017). Read more (PubMed: 28409281) »
- Schmouth JF et al. Non-coding-regulatory regions of human brain genes delineated by bacterial artificial chromosome knock-in mice. BMC Biol 11:106 (2013). IHC . Read more (PubMed: 24124870) »
