Anti-Calbindin antibody (ab49899)
Key features and details
- Rabbit polyclonal to Calbindin
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Calbindin antibody
See all Calbindin primary antibodies -
Description
Rabbit polyclonal to Calbindin -
Host species
Rabbit -
Specificity
We recommend the use of RabMAb ab108404 for testing in IHC and ICC/IF for this target. -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Hamster, Cow, Pig
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Calbindin.
Read Abcam's proprietary immunogen policy (Peptide available as ab60452.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab49899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 30 kDa). |
Target
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Function
Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. -
Sequence similarities
Belongs to the calbindin family.
Contains 5 EF-hand domains. -
Domain
This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium. - Information by UniProt
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Database links
- Entrez Gene: 539930 Cow
- Entrez Gene: 793 Human
- Entrez Gene: 12307 Mouse
- Entrez Gene: 100153645 Pig
- Entrez Gene: 83839 Rat
- Omim: 114050 Human
- SwissProt: P04467 Cow
- SwissProt: P05937 Human
see all -
Alternative names
- avian-type antibody
- CAB27 antibody
- CALB 1 antibody
see all
Images
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Western blot - Anti-Calbindin antibody (ab49899)All lanes : ab49899 at 1 µg/ml
Lane 1 : Brain (human) tissue lysate
Lane 2 : Brain (Mouse) Tissue lysate
Lane 3 : Brain (Rat) Tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 37 kDa, 51 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
Western blot - Anti-Calbindin antibody (ab49899)All lanes : Anti-Calbindin antibody (ab49899) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 150 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-Calbindin antibody (ab49899)Calbindin - Neuronal Marker was immunoprecipitated using 0.5mg Mouse kidney whole tissue extract, 5µg of Rabbit polyclonal to Calbindin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse kidney whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab49899.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 30kDa: Calbindin - Neuronal Marker.
Protocols
Datasheets and documents
References (2)
ab49899 has been referenced in 2 publications.
- Schludi MH et al. Spinal poly-GA inclusions in a C9orf72 mouse model trigger motor deficits and inflammation without neuron loss. Acta Neuropathol 134:241-254 (2017). PubMed: 28409281
- Schmouth JF et al. Non-coding-regulatory regions of human brain genes delineated by bacterial artificial chromosome knock-in mice. BMC Biol 11:106 (2013). IHC . PubMed: 24124870
