Anti-Calbindin antibody [EP3478] (ab108404)
- Datasheet
- Specific References (2)
- Protocols
Overview
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Product nameAnti-Calbindin antibody [EP3478]
See all Calbindin primary antibodies -
DescriptionRabbit monoclonal [EP3478] to Calbindin
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IHC-Frmore details
Unsuitable for: Flow Cyt or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Calbindin aa 50-150 (internal sequence). The exact sequence is proprietary.
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Positive control
- Fetal brain, fetal cerebellum, fetal hypothalamus and fetal pituitary lysates; Human brain tissue
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP3478
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab108404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 30 kDa. | |
| IHC-P | 1/100 - 1/250. Antigen retrieval is recommended. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | 1/100. | |
| IHC-Fr | 1/500. |
Target
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FunctionBuffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
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Sequence similaritiesBelongs to the calbindin family.
Contains 5 EF-hand domains. -
DomainThis protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.
- Information by UniProt
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Database links
- Entrez Gene: 793 Human
- Entrez Gene: 12307 Mouse
- Entrez Gene: 83839 Rat
- Omim: 114050 Human
- SwissProt: P05937 Human
- SwissProt: P12658 Mouse
- SwissProt: P07171 Rat
- Unigene: 65425 Human
see all -
Alternative names
- avian-type antibody
- CAB27 antibody
- CALB 1 antibody
see all
Images
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![Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (ab108404)](https://www.abcam.com/ps/products/108/ab108404/Images/ab108404-263175-ab108404IF-Frozen.jpg)
Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (ab108404)Immunohistochemical analysis of mouse frozen cerebellum tissue sections with ab108404 labelling Calbindin at 1/500. Sections were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue). The image shows specific staining on Purkinje cells of mouse cerebellum.
Secondary antibody only was used as negative control and is shown in the inset.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)](https://www.abcam.com/ps/products/108/ab108404/Images/Calbindin-Primary-antibodies-ab108404-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)ab108404 showing positive staining in Normal kidney tissue. -
![Western blot - Anti-Calbindin antibody [EP3478] (ab108404)](https://www.abcam.com/ps/products/108/ab108404/Images/Calbindin-Primary-antibodies-ab108404-2.gif)
Western blot - Anti-Calbindin antibody [EP3478] (ab108404)All lanes : Anti-Calbindin antibody [EP3478] (ab108404) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Fetal cerebellum lysate
Lane 3 : Fetal hypothalamus lysate
Lane 4 : Fetal pituitary lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 30 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Calbindin antibody [EP3478] (ab108404)](https://www.abcam.com/ps/products/108/ab108404/Images/ab108404-263181-ab108404-ICCIF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Calbindin antibody [EP3478] (ab108404)Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) cells labelling Calbindin with ab108404 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at dilution of 1/200. Nuclei were counterstained with DAPI (blue).
Confocal image showing nuclear and cytoplasmic staining on Neuro2a cultured with 2% FBS.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)](https://www.abcam.com/ps/products/108/ab108404/Images/Calbindin-Primary-antibodies-ab108404-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)ab108404, at 1/100 dilution, staining Calbindin in paraffin-embedded Human brain tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)](https://www.abcam.com/ps/products/108/ab108404/Images/Calbindin-Primary-antibodies-ab108404-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)ab108404 showing negative staining in Normal heart tissue. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)](https://www.abcam.com/ps/products/108/ab108404/Images/Calbindin-Primary-antibodies-ab108404-6.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)ab108404 showing negative staining in Normal colon tissue. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)](https://www.abcam.com/ps/products/108/ab108404/Images/Calbindin-Primary-antibodies-ab108404-7.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)ab108404 showing negative staining in Normal liver tissue.
Protocols
References
This product has been referenced in:
- Dietrich P et al. Elimination of huntingtin in the adult mouse leads to progressive behavioral deficits, bilateral thalamic calcification, and altered brain iron homeostasis. PLoS Genet 13:e1006846 (2017). Read more (PubMed: 28715425) »
- McMahon SM et al. Multiple cytosolic calcium buffers in posterior pituitary nerve terminals. J Gen Physiol 147:243-54 (2016). WB ; Rat . Read more (PubMed: 26880753) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"