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Neuroscience Neurotransmission Calcium Signaling Calcium Binding Proteins
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RecombinantRabMAb

Recombinant Anti-Calbindin antibody [EP3478] (ab108404)

  • Datasheet
Reviews (3) Submit a question References (9)

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Western blot - Anti-Calbindin antibody [EP3478] (ab108404)
  • Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (ab108404)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
  • Western blot - Anti-Calbindin antibody [EP3478] (ab108404)
  • Flow Cytometry - Anti-Calbindin antibody [EP3478] (ab108404)
  • Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (ab108404)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calbindin antibody [EP3478] (ab108404)
  • Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (ab108404)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calbindin antibody [EP3478] (ab108404)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
  • Anti-Calbindin antibody [EP3478] (ab108404)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3478] to Calbindin
  • Suitable for: WB, IHC-P, ICC/IF, IHC-Fr, Flow Cyt
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Calbindin antibody [EP3478]
    See all Calbindin primary antibodies
  • Description

    Rabbit monoclonal [EP3478] to Calbindin
  • Host species

    Rabbit
  • Specificity

    We do not guarantee IHC-P for mouse and rat.

    We do not guarantee IHC-Fr for human.

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IHC-Fr, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Calbindin aa 50-150 (internal sequence). The exact sequence is proprietary.

  • Positive control

    • WB: Recombinant Human Calbindin protein (ab117207), fetal brain, fetal cerebellum, fetal hypothalamus and fetal pituitary lysates, SH-SY5Y, Human kidney, Mouse brain, Mouse kidney, and Rat brain lysates. IHC-P: Human cerebrum and Human brain tissue. IHC-Fr: Mouse and Rat cerebellum tissues. ICC/IF: SH-SY5Y cells. Flow Cyt: SH-SY5Y cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP3478
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Binding Proteins
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-Calbindin antibody [EP3478] (ab207528)
    • Alexa Fluor® 488 Anti-Calbindin antibody [EP3478] (ab208377)
    • Anti-Calbindin antibody [EP3478] - BSA and Azide free (ab233018)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Calbindin protein (ab82631)

Applications

Our Abpromise guarantee covers the use of ab108404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols

For unpurified use at 1/100 - 1/250. 

ICC/IF 1/150.

For unpurified use at 1/100

IHC-Fr 1/500.

We do not guarantee IHC-Fr for human

Flow Cyt 1/150.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
    • Sequence similarities

      Belongs to the calbindin family.
      Contains 5 EF-hand domains.
    • Domain

      This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.
    • Target information above from: UniProt accession P05937 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 793 Human
      • Entrez Gene: 12307 Mouse
      • Entrez Gene: 83839 Rat
      • Omim: 114050 Human
      • SwissProt: P05937 Human
      • SwissProt: P12658 Mouse
      • SwissProt: P07171 Rat
      • Unigene: 65425 Human
      • Unigene: 277665 Mouse
      • Unigene: 3908 Rat
      see all
    • Alternative names

      • avian-type antibody
      • CAB27 antibody
      • CALB 1 antibody
      • CALB antibody
      • CALB1 antibody
      • CALB1_HUMAN antibody
      • Calbindin 1 28kDa antibody
      • Calbindin antibody
      • Calbindin D28 antibody
      • D 28K antibody
      • D-28K antibody
      • D28K antibody
      • OTTHUMP00000166027 antibody
      • OTTHUMP00000225441 antibody
      • RTVL H protein antibody
      • Vitamin D dependent calcium binding protein antibody
      • Vitamin D dependent calcium binding protein avian type antibody
      • Vitamin D-dependent calcium-binding protein antibody
      see all

    Images

    • Western blot - Anti-Calbindin antibody [EP3478] (ab108404)
      Western blot - Anti-Calbindin antibody [EP3478] (ab108404)
      All lanes : Anti-Calbindin antibody [EP3478] (ab108404) at 1/2000 dilution (Purified)

      Lane 1 : Human fetal brain lysates
      Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
      Lane 3 : Human kidney lysates
      Lane 4 : Mouse brain lysates
      Lane 5 : Mouse kidney lysates
      Lane 6 : Rat brain lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 30 kDa
      Observed band size: 30 kDa

    • Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (ab108404)
      Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (ab108404)
      Immunohistochemistry (Frozen) analysis of mouse cerebellum tissue sections labeling Calbindin with purified ab108404 at 1/500 dilution (3.0 µg/ml). The tissue section was fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.
      Secondary antibody only control: PBS instead of the primary antibody
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling Calbindin with purified ab108404 at 1/1000 dilution (1.49 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Western blot - Anti-Calbindin antibody [EP3478] (ab108404)
      Western blot - Anti-Calbindin antibody [EP3478] (ab108404)
      All lanes : Anti-Calbindin antibody [EP3478] (ab108404) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Fetal cerebellum lysate
      Lane 3 : Fetal hypothalamus lysate
      Lane 4 : Fetal pituitary lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 30 kDa

    • Flow Cytometry - Anti-Calbindin antibody [EP3478] (ab108404)
      Flow Cytometry - Anti-Calbindin antibody [EP3478] (ab108404)
      Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Calbindin with purified ab108404 at 1/150 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    • Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (ab108404)
      Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (ab108404)
      Immunohistochemistry (Frozen) analysis of rat cerebellum tissue sections labeling Calbindin with purified ab108404 at 1/500 dilution (3.0 µg/ml). The tissue section was fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.
      Secondary antibody only control: PBS instead of the primary antibody
    • Immunocytochemistry/ Immunofluorescence - Anti-Calbindin antibody [EP3478] (ab108404)
      Immunocytochemistry/ Immunofluorescence - Anti-Calbindin antibody [EP3478] (ab108404)
      Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Calbindin with purified ab108404 at 1/150 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (ab108404)
      Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (ab108404)

      Immunohistochemical analysis of mouse frozen cerebellum tissue sections with ab108404 labelling Calbindin at 1/500. Sections were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).  The image shows specific staining on Purkinje cells of mouse cerebellum.

      Secondary antibody only was used as negative control and is shown in the inset.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
      ab108404 showing positive staining in Normal kidney tissue.

    • Immunocytochemistry/ Immunofluorescence - Anti-Calbindin antibody [EP3478] (ab108404)
      Immunocytochemistry/ Immunofluorescence - Anti-Calbindin antibody [EP3478] (ab108404)

      Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) cells labelling Calbindin with ab108404 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at dilution of 1/200. Nuclei were counterstained with DAPI (blue).

      Confocal image showing nuclear and cytoplasmic staining on Neuro2a cultured with 2% FBS.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
      ab108404, at 1/100 dilution, staining Calbindin in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
      ab108404 showing negative staining in Normal heart tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
      ab108404 showing negative staining in Normal colon tissue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)
      ab108404 showing negative staining in Normal liver tissue.

    • Anti-Calbindin antibody [EP3478] (ab108404)
      Anti-Calbindin antibody [EP3478] (ab108404)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (9)

    Publishing research using ab108404? Please let us know so that we can cite the reference in this datasheet.

    ab108404 has been referenced in 9 publications.

    • Chen B  et al. A C1976Y missense mutation in the mouse Ip3r1 gene leads to short-term mydriasis and unfolded protein response in the iris constrictor muscles. Exp Anim 69:45-53 (2020). PubMed: 31391379
    • Dafinca R  et al. Impairment of Mitochondrial Calcium Buffering Links Mutations in C9ORF72 and TARDBP in iPS-Derived Motor Neurons from Patients with ALS/FTD. Stem Cell Reports 14:892-908 (2020). PubMed: 32330447
    • Béchet S  et al. Fingolimod Rescues Demyelination in a Mouse Model of Krabbe's Disease. J Neurosci 40:3104-3118 (2020). PubMed: 32127495
    • Salimi H  et al. Encephalitic Alphaviruses Exploit Caveola-Mediated Transcytosis at the Blood-Brain Barrier for Central Nervous System Entry. mBio 11:N/A (2020). PubMed: 32047126
    • Qin J  et al. Early Forebrain Neurons and Scaffold Fibers in Human Embryos. Cereb Cortex N/A:N/A (2019). PubMed: 31298263
    • Cao LQ  et al. CALB1 enhances the interaction between p53 and MDM2, and inhibits the senescence of ovarian cancer cells. Mol Med Rep 19:5097-5104 (2019). PubMed: 31059057
    • Dietrich P  et al. Elimination of huntingtin in the adult mouse leads to progressive behavioral deficits, bilateral thalamic calcification, and altered brain iron homeostasis. PLoS Genet 13:e1006846 (2017). PubMed: 28715425
    • Nuryyev RL  et al. Transplantation of Human Neural Progenitor Cells Reveals Structural and Functional Improvements in the Spastic Han-Wistar Rat Model of Ataxia. Cell Transplant 26:1811-1821 (2017). PubMed: 29338380
    • McMahon SM  et al. Multiple cytosolic calcium buffers in posterior pituitary nerve terminals. J Gen Physiol 147:243-54 (2016). WB ; Rat . PubMed: 26880753

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-3 of 3 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-Calbindin antibody [EP3478]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Kidney)
    Specification
    Kidney
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 29 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calbindin antibody [EP3478]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (macaque retina)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate pH 6
    Permeabilization
    No
    Specification
    macaque retina
    Blocking step
    Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 06 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-Calbindin antibody [EP3478]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Fukomys anselli Tissue sections (Retina)
    Permeabilization
    Yes - Triton X-100 in Blocking Solution
    Specification
    Retina
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative
    Davidson's Fixative
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 28 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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