Name: Anti-Calbindin antibody [EP3478]
Brand: RabMAb
SKU: ab108404
Rating: 4 (3 reviews)

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Western blot - Anti-Calbindin antibody [EP3478] (ab108404)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP3478] to Calbindin
Suitable for: WB, IHC-P, ICC/IF, IHC-Fr, Flow Cyt (Intra)
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

Product name

Anti-Calbindin antibody [EP3478]
See all Calbindin primary antibodies
Description

Rabbit monoclonal [EP3478] to Calbindin
Host species

Rabbit
Specificity

We do not guarantee IHC-P for mouse and rat.

We do not guarantee IHC-Fr for human.
Tested applications

Suitable for: WB, IHC-P, ICC/IF, IHC-Fr, Flow Cyt (Intra)more details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Recombinant Human Calbindin protein (ab117207), fetal brain, fetal cerebellum, fetal hypothalamus and fetal pituitary lysates, SH-SY5Y, Human kidney, Mouse brain, Mouse kidney, and Rat brain lysates. IHC-P: Human cerebrum and Human brain tissue. IHC-Fr: Mouse and Rat cerebellum tissues. ICC/IF: SH-SY5Y cells. Flow Cyt (intra): SH-SY5Y cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP3478
Isotype

IgG
Research areas
- Neuroscience
- Processes

Alternative Versions
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
IHC-P	(1)	1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. We do not guarantee IHC-P for mouse and rat. See IHC antigen retrieval protocols For unpurified use at 1/100 - 1/250.
ICC/IF		1/150. For unpurified use at 1/100
IHC-Fr	(1)	1/500. We do not guarantee IHC-Fr for human
Flow Cyt (Intra)		1/150.

Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. We do not guarantee IHC-P for mouse and rat. See IHC antigen retrieval protocols For unpurified use at 1/100 - 1/250.
ICC/IF 1/150. For unpurified use at 1/100
IHC-Fr 1/500. We do not guarantee IHC-Fr for human
Flow Cyt (Intra) 1/150.

Application notes

Is unsuitable for IP.

Function

Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
Sequence similarities

Belongs to the calbindin family.
Contains 5 EF-hand domains.
Domain

This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.
Target information above from: UniProt accession P05937 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 793 Human
- Entrez Gene: 12307 Mouse
- Entrez Gene: 83839 Rat
- Omim: 114050 Human
- SwissProt: P05937 Human
- SwissProt: P12658 Mouse
- SwissProt: P07171 Rat
- Unigene: 65425 Human
- Unigene: 277665 Mouse
- Unigene: 3908 Rat
see all
Alternative names
- avian-type antibody
- CAB27 antibody
- CALB 1 antibody
- CALB antibody
- CALB1 antibody
- CALB1_HUMAN antibody
- Calbindin 1 28kDa antibody
- Calbindin antibody
- Calbindin D28 antibody
- D 28K antibody
- D-28K antibody
- D28K antibody
- OTTHUMP00000166027 antibody
- OTTHUMP00000225441 antibody
- RTVL H protein antibody
- Vitamin D dependent calcium binding protein antibody
- Vitamin D dependent calcium binding protein avian type antibody
- Vitamin D-dependent calcium-binding protein antibody
see all

Western blot - Anti-Calbindin antibody [EP3478] (ab108404)

All lanes : Anti-Calbindin antibody [EP3478] (ab108404) at 1/2000 dilution (Purified)

Lane 1 : Human fetal brain lysates
Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
Lane 3 : Human kidney lysates
Lane 4 : Mouse brain lysates
Lane 5 : Mouse kidney lysates
Lane 6 : Rat brain lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 30 kDa
Observed band size: 30 kDa
Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (ab108404)

Immunohistochemistry (Frozen) analysis of mouse cerebellum tissue sections labeling Calbindin with purified ab108404 at 1/500 dilution (3.0 µg/ml). The tissue section was fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.
Secondary antibody only control: PBS instead of the primary antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling Calbindin with purified ab108404 at 1/1000 dilution (1.49 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-Calbindin antibody [EP3478] (ab108404)

All lanes : Anti-Calbindin antibody [EP3478] (ab108404) at 1/1000 dilution

Lane 1 : Fetal brain lysate
Lane 2 : Fetal cerebellum lysate
Lane 3 : Fetal hypothalamus lysate
Lane 4 : Fetal pituitary lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 30 kDa
Flow Cytometry (Intracellular) - Anti-Calbindin antibody [EP3478] (ab108404)

Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Calbindin with purified ab108404 at 1/150 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (ab108404)

Immunohistochemistry (Frozen) analysis of rat cerebellum tissue sections labeling Calbindin with purified ab108404 at 1/500 dilution (3.0 µg/ml). The tissue section was fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.
Secondary antibody only control: PBS instead of the primary antibody
Immunocytochemistry/ Immunofluorescence - Anti-Calbindin antibody [EP3478] (ab108404)

Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Calbindin with purified ab108404 at 1/150 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (ab108404)

Immunohistochemical analysis of mouse frozen cerebellum tissue sections with ab108404 labelling Calbindin at 1/500. Sections were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue). The image shows specific staining on Purkinje cells of mouse cerebellum.

Secondary antibody only was used as negative control and is shown in the inset.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)

ab108404 showing positive staining in Normal kidney tissue.
Immunocytochemistry/ Immunofluorescence - Anti-Calbindin antibody [EP3478] (ab108404)

Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) cells labelling Calbindin with ab108404 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at dilution of 1/200. Nuclei were counterstained with DAPI (blue).

Confocal image showing nuclear and cytoplasmic staining on Neuro2a cultured with 2% FBS.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)

ab108404, at 1/100 dilution, staining Calbindin in paraffin-embedded Human brain tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)

ab108404 showing negative staining in Normal heart tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)

ab108404 showing negative staining in Normal colon tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (ab108404)

ab108404 showing negative staining in Normal liver tissue.
Anti-Calbindin antibody [EP3478] (ab108404)

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab108404? Please let us know so that we can cite the reference in this datasheet.

ab108404 has been referenced in 10 publications.

Striebel JF et al. Prion-induced photoreceptor degeneration begins with misfolded prion protein accumulation in cones at two distinct sites: cilia and ribbon synapses. Acta Neuropathol Commun 9:17 (2021). PubMed: 33509294
Chen B et al. A C1976Y missense mutation in the mouse Ip3r1 gene leads to short-term mydriasis and unfolded protein response in the iris constrictor muscles. Exp Anim 69:45-53 (2020). PubMed: 31391379
Dafinca R et al. Impairment of Mitochondrial Calcium Buffering Links Mutations in C9ORF72 and TARDBP in iPS-Derived Motor Neurons from Patients with ALS/FTD. Stem Cell Reports 14:892-908 (2020). PubMed: 32330447
Béchet S et al. Fingolimod Rescues Demyelination in a Mouse Model of Krabbe's Disease. J Neurosci 40:3104-3118 (2020). PubMed: 32127495
Salimi H et al. Encephalitic Alphaviruses Exploit Caveola-Mediated Transcytosis at the Blood-Brain Barrier for Central Nervous System Entry. mBio 11:N/A (2020). PubMed: 32047126
Qin J et al. Early Forebrain Neurons and Scaffold Fibers in Human Embryos. Cereb Cortex N/A:N/A (2019). PubMed: 31298263
Cao LQ et al. CALB1 enhances the interaction between p53 and MDM2, and inhibits the senescence of ovarian cancer cells. Mol Med Rep 19:5097-5104 (2019). PubMed: 31059057
Dietrich P et al. Elimination of huntingtin in the adult mouse leads to progressive behavioral deficits, bilateral thalamic calcification, and altered brain iron homeostasis. PLoS Genet 13:e1006846 (2017). PubMed: 28715425
Nuryyev RL et al. Transplantation of Human Neural Progenitor Cells Reveals Structural and Functional Improvements in the Spastic Han-Wistar Rat Model of Ataxia. Cell Transplant 26:1811-1821 (2017). PubMed: 29338380
McMahon SM et al. Multiple cytosolic calcium buffers in posterior pituitary nerve terminals. J Gen Physiol 147:243-54 (2016). WB ; Rat . PubMed: 26880753

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Application

IHC - Wholemount

Sample

Mouse Tissue (Kidney)

Specification

Kidney

Abcam user community

Verified customer

Submitted Sep 29 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rhesus monkey Tissue sections (macaque retina)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate pH 6

Permeabilization

Specification

macaque retina

Blocking step

Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Nov 06 2018

Application

Immunohistochemistry (Frozen sections)

Sample

Fukomys anselli Tissue sections (Retina)

Permeabilization

Yes - Triton X-100 in Blocking Solution

Specification

Retina

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C

Fixative

Davidson's Fixative

Abcam user community

Verified customer

Submitted Jul 28 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Domain

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (10)

Customer reviews and Q&As

IHC - Wholemount abreview for Anti-Calbindin antibody [EP3478]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calbindin antibody [EP3478]

Immunohistochemistry (Frozen sections) abreview for Anti-Calbindin antibody [EP3478]