Calcein AM, fluorescent dye for cell viability (ab141420)
- Datasheet
- References (1)
Overview
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Product name
Calcein AM, fluorescent dye for cell viability -
Description
Cell-permeable fluorescent dye for determining cell viability -
Purity
> 98% -
CAS Number
148504-34-1 -
Chemical structure
Properties
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Excitation
495nm -
Emission
515nm -
Chemical name
N,N'-[[3',6'-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-
[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-glycine 1,1'-bis[(acetyloxy)methyl] ester -
Molecular weight
994.87 -
Molecular formula
C46H46N2O23 -
PubChem identifier
390986 -
Storage instructions
Store at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions. -
Solubility overview
Soluble in DMSO to 5 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
For more information on AM esters please visit our AM esters FAQ page.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC(=O)OCOC(=O)CN(CC1=C(C=C2C(=C1)C3(C4=CC=CC=C4C(=O)O3)C5=CC(=C(C=C5O2)OC(=O)C)CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OC(=O)C)CC(=O)OCOC(=O)C -
Source
Synthetic
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Research areas
References
This product has been referenced in:
- Rioult D et al. The multi-xenobiotic resistance (MXR) efflux activity in hemocytes of Mytilus edulis is mediated by an ATP binding cassette transporter of class C (ABCC) principally inducible in eosinophilic granulocytes. Aquat Toxicol : (2013). Read more (PubMed: 24345773) »
- Pasquier J et al. P-Glycoprotein-Activity Measurements in Multidrug Resistant Cell Lines: Single-Cell versus Single-Well Population Fluorescence Methods. Biomed Res Int 2013:676845 (2013). Read more (PubMed: 24350282) »
- Bratosin D et al. Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging. Cytometry A 66:78-84 (2005). Read more (PubMed: 15915509) »
