Calcein AM, fluorescent dye for cell viability (ab141420)
- Datasheet
- References (1)
Overview
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Product nameCalcein AM, fluorescent dye for cell viability
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DescriptionCell-permeable fluorescent dye for determining cell viability
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Biological description
Cell-permeable fluorescent dye for determining cell viability. Hydrolyzes to green-fluorescent calcein on contact with intracellular esterases in live cells. Self-quenches at concentrations below 100 mM.
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Purity> 98%
Properties
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Excitation495nm
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Emission515nm
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Chemical nameN,N'-[[3',6'-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-
[9H]xanthene]-2',7'-diyl]bis(methylene)]bis[N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-glycine 1,1'-bis[(acetyloxy)methyl] ester -
Molecular weight994.87
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Chemical structure
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Molecular formulaC46H46N2O23
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CAS Number148504-34-1
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PubChem identifier390986
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Storage instructionsStore at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions.
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Solubility overviewSoluble in DMSO to 5 mM
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
For more information on AM esters please visit our AM esters FAQ page.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILESCC(=O)OCOC(=O)CN(CC1=C(C=C2C(=C1)C3(C4=CC=CC=C4C(=O)O3)C5=CC(=C(C=C5O2)OC(=O)C)CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OC(=O)C)CC(=O)OCOC(=O)C
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Source
Synthetic
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Research areas
References
This product has been referenced in:
- Rioult D et al. The multi-xenobiotic resistance (MXR) efflux activity in hemocytes of Mytilus edulis is mediated by an ATP binding cassette transporter of class C (ABCC) principally inducible in eosinophilic granulocytes. Aquat Toxicol : (2013). Read more (PubMed: 24345773) »
- Pasquier J et al. P-Glycoprotein-Activity Measurements in Multidrug Resistant Cell Lines: Single-Cell versus Single-Well Population Fluorescence Methods. Biomed Res Int 2013:676845 (2013). Read more (PubMed: 24350282) »
- Bratosin D et al. Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging. Cytometry A 66:78-84 (2005). Read more (PubMed: 15915509) »
