Overview

  • Product name

    Anti-Calcineurin A antibody
    See all Calcineurin A primary antibodies
  • Description

    Rabbit polyclonal to Calcineurin A
  • Host species

    Rabbit
  • Specificity

    ab137335 recognizes all isoforms of Calcineurin A, based on immunogen sequence.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 59-296 of Human Calcineurin A.

  • Positive control

    • Recombinant Human Calcineurin A protein (ab101870) can be used as a positive control in WB. MOLT4, mouse brain whole cell lysate; 293T, A431, H1299, HeLa, HepG2, Raji cell lines; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab137335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 59 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.
  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-2B subfamily.
  • Cellular localization

    Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha isoform formerly PPP2B antibody
    • Calcineurin A alpha antibody
    • Calcineurin A1 antibody
    • CalcineurinA antibody
    • Calmodulin dependent calcineurin A subunit alpha isoform antibody
    • Calmodulin-dependent calcineurin A subunit alpha isoform antibody
    • CALN antibody
    • CALNA 1 antibody
    • CALNA antibody
    • CALNA1 antibody
    • CAM PRP catalytic subunit antibody
    • CAM-PRP catalytic subunit antibody
    • CCN 1 antibody
    • CCN1 antibody
    • CNA 1 antibody
    • CNA alpha antibody
    • CNA antibody
    • CNA1 antibody
    • PP2BA_HUMAN antibody
    • PPP2B antibody
    • Ppp3ca antibody
    • Protein phosphatase 2B catalytic subunit antibody
    • Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody
    • Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody
    • Protein phosphatase 3 catalytic subunit alpha isozyme antibody
    • Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody
    • Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody
    see all

Images

  • Anti-Calcineurin A antibody (ab137335) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 59 kDa



    7.5% SDS PAGE
  • Anti-Calcineurin A antibody (ab137335) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 59 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling Calcineurin A, using ab137335 (green) at a 1/200 dilution. Cell nuclei are shown in blue.

References

This product has been referenced in:

  • Marciel MP  et al. Selenoprotein K deficiency inhibits melanoma by reducing calcium flux required for tumor growth and metastasis. Oncotarget 9:13407-13422 (2018). Read more (PubMed: 29568366) »
  • Silverman-Gavrila LB  et al. Calcium, calpain, and calcineurin in low-frequency depression of transmitter release. J Neurosci 33:1975-90 (2013). Read more (PubMed: 23365236) »
See all 2 Publications for this product

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