For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    calcineurin-a-antibody-ab240913.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Adaptive Immunity B Cells Non-CD
Share by email

Anti-Calcineurin A antibody (ab240913)

  • Datasheet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Calcineurin A antibody (ab240913)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody (ab240913)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody (ab240913)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody (ab240913)

Key features and details

  • Rabbit polyclonal to Calcineurin A
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human Calcineurin A protein (ab101870)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Knockout
Product image
Human PPP3CA (Calcineurin A) knockout HeLa cell lysate (ab257181)

View more associated products

Overview

  • Product name

    Anti-Calcineurin A antibody
    See all Calcineurin A primary antibodies
  • Description

    Rabbit polyclonal to Calcineurin A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Dog
  • Immunogen

    Synthetic peptide corresponding to Human Calcineurin A aa 264-283.
    Database link: Q08209

  • Positive control

    • ICC/IF: HeLa cells. IHC-P: Mouse backskin tissue. WB: Rat brain lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • Non-CD
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calmodulin Pathway

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Calcineurin A protein (ab101870)
  • Related Products

    • Recombinant Human Calcineurin A protein (ab101870)
    • Recombinant Human Calcineurin A protein (ab128554) (ab128554)

Applications

Our Abpromise guarantee covers the use of ab240913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB 1/1000.
ICC/IF 1/120.

Target

  • Function

    Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.
  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-2B subfamily.
  • Cellular localization

    Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
  • Target information above from: UniProt accession Q08209 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5530 Human
    • Entrez Gene: 19055 Mouse
    • Entrez Gene: 24674 Rat
    • Omim: 114105 Human
    • SwissProt: Q08209 Human
    • SwissProt: P63328 Mouse
    • SwissProt: P63329 Rat
    • Unigene: 435512 Human
    • Unigene: 331389 Mouse
    • Unigene: 6866 Rat
    see all
  • Alternative names

    • Alpha isoform formerly PPP2B antibody
    • Calcineurin A alpha antibody
    • Calcineurin A1 antibody
    • CalcineurinA antibody
    • Calmodulin dependent calcineurin A subunit alpha isoform antibody
    • Calmodulin-dependent calcineurin A subunit alpha isoform antibody
    • CALN antibody
    • CALNA 1 antibody
    • CALNA antibody
    • CALNA1 antibody
    • CAM PRP catalytic subunit antibody
    • CAM-PRP catalytic subunit antibody
    • CCN 1 antibody
    • CCN1 antibody
    • CNA 1 antibody
    • CNA alpha antibody
    • CNA antibody
    • CNA1 antibody
    • PP2BA_HUMAN antibody
    • PPP2B antibody
    • Ppp3ca antibody
    • Protein phosphatase 2B catalytic subunit antibody
    • Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody
    • Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody
    • Protein phosphatase 3 catalytic subunit alpha isozyme antibody
    • Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody
    • Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody
    see all

Images

  • Western blot - Anti-Calcineurin A antibody (ab240913)
    Western blot - Anti-Calcineurin A antibody (ab240913)
    Anti-Calcineurin A antibody (ab240913) at 1/1000 dilution + Rat brain lysate at 15 µg

    Secondary
    HRP-linked goat anti-rabbit IgG at 1/2000 dilution

    Developed using the ECL technique.

    Exposure time: 5 minutes


    Blocked with 5% skim milk in 1XTBST.

    Primary incubation: 2 hours at RT.

    Secondary incubation: 1 hour RT.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody (ab240913)
    Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody (ab240913)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Calcineurin A (green) using ab240913 at 1/120 dilution in ICC/IF, followed by a FITC conjugated goat anti-rabbit IgG at 1/200 dilution. Cells were fixed using 2% formaldehyde for 20 minutes at room temperature. Primary antibody was incubated for 12 hours at 4°C and secondary antibody for 2 hours at room temperature. Counterstained with DAPI (blue) at a 1/40000 dilution, for 2 hours at room temperature.

    (A) DAPI (blue)

    (B) ab240913 labeling Calcineurin A (green)

    (C) Composite.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody (ab240913)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody (ab240913)

    Mouse backskin tissue fixed with Bouin's fixative solution, stained for Calcineurin A using ab240913 at 1/100 dilution (1 hour at RT) in immunohistochemical analysis. Secondary antibody was a FITC cnjugated goat anti-rabbit IgG (green) at a 1/50 dilution incubated for 1 hour at RT.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody (ab240913)
    Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody (ab240913)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Calcineurin A (green) using ab240913 at 1/120 dilution in ICC/IF, followed by a APC conjugated goat anti-rabbit IgG at 1/200 dilution. Cells were fixed using 2% formaldehyde for 20 minutes at room temperature. Primary antibody was incubated for 12 hours at 4°C and secondary antibody for 2 hours at room temperature. Counterstained with DAPI (blue) at a 1/40000 dilution, for 2 hours at room temperature.

    (A) DAPI (blue)

    (B) ab240913 labeling Calcineurin A (red)

    (C) Composite.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab240913? Please let us know so that we can cite the reference in this datasheet.

    ab240913 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab240913.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.