Anti-Calcineurin A antibody (ab240913)
Key features and details
- Rabbit polyclonal to Calcineurin A
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Calcineurin A antibody
See all Calcineurin A primary antibodies -
Description
Rabbit polyclonal to Calcineurin A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Dog
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Immunogen
Synthetic peptide corresponding to Human Calcineurin A aa 264-283.
Database link: Q08209 -
Positive control
- ICC/IF: HeLa cells. IHC-P: Mouse backskin tissue. WB: Rat brain lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab240913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. | |
| WB | 1/1000. | |
| ICC/IF | 1/120. |
Target
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Function
Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1. -
Sequence similarities
Belongs to the PPP phosphatase family. PP-2B subfamily. -
Cellular localization
Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle. - Information by UniProt
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Database links
- Entrez Gene: 5530 Human
- Entrez Gene: 19055 Mouse
- Entrez Gene: 24674 Rat
- Omim: 114105 Human
- SwissProt: Q08209 Human
- SwissProt: P63328 Mouse
- SwissProt: P63329 Rat
- Unigene: 435512 Human
see all -
Alternative names
- Alpha isoform formerly PPP2B antibody
- Calcineurin A alpha antibody
- Calcineurin A1 antibody
see all
Images
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Western blot - Anti-Calcineurin A antibody (ab240913)Anti-Calcineurin A antibody (ab240913) at 1/1000 dilution + Rat brain lysate at 15 µg
Secondary
HRP-linked goat anti-rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutesBlocked with 5% skim milk in 1XTBST.
Primary incubation: 2 hours at RT.
Secondary incubation: 1 hour RT.
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Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody (ab240913)HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Calcineurin A (green) using ab240913 at 1/120 dilution in ICC/IF, followed by a FITC conjugated goat anti-rabbit IgG at 1/200 dilution. Cells were fixed using 2% formaldehyde for 20 minutes at room temperature. Primary antibody was incubated for 12 hours at 4°C and secondary antibody for 2 hours at room temperature. Counterstained with DAPI (blue) at a 1/40000 dilution, for 2 hours at room temperature.
(A) DAPI (blue)
(B) ab240913 labeling Calcineurin A (green)
(C) Composite.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody (ab240913)Mouse backskin tissue fixed with Bouin's fixative solution, stained for Calcineurin A using ab240913 at 1/100 dilution (1 hour at RT) in immunohistochemical analysis. Secondary antibody was a FITC cnjugated goat anti-rabbit IgG (green) at a 1/50 dilution incubated for 1 hour at RT.
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Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody (ab240913)HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Calcineurin A (green) using ab240913 at 1/120 dilution in ICC/IF, followed by a APC conjugated goat anti-rabbit IgG at 1/200 dilution. Cells were fixed using 2% formaldehyde for 20 minutes at room temperature. Primary antibody was incubated for 12 hours at 4°C and secondary antibody for 2 hours at room temperature. Counterstained with DAPI (blue) at a 1/40000 dilution, for 2 hours at room temperature.
(A) DAPI (blue)
(B) ab240913 labeling Calcineurin A (red)
(C) Composite.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab240913 has not yet been referenced specifically in any publications.
