Anti-Calcineurin A antibody (ab240913)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Calcineurin A antibody
See all Calcineurin A primary antibodies -
Description
Rabbit polyclonal to Calcineurin A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Dog
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Immunogen
Synthetic peptide corresponding to Human Calcineurin A aa 264-283.
Database link: Q08209 -
Positive control
- ICC/IF: HeLa cells. IHC-P: Mouse backskin tissue. WB: Rat brain lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab240913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. | |
| WB | 1/1000. | |
| ICC/IF | 1/120. |
Target
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Function
Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1. -
Sequence similarities
Belongs to the PPP phosphatase family. PP-2B subfamily. -
Cellular localization
Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle. - Information by UniProt
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Database links
- Entrez Gene: 5530 Human
- Entrez Gene: 19055 Mouse
- Entrez Gene: 24674 Rat
- Omim: 114105 Human
- SwissProt: Q08209 Human
- SwissProt: P63328 Mouse
- SwissProt: P63329 Rat
- Unigene: 435512 Human
see all -
Alternative names
- Alpha isoform formerly PPP2B antibody
- Calcineurin A alpha antibody
- Calcineurin A1 antibody
see all
Images
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Western blot - Anti-Calcineurin A antibody (ab240913)Anti-Calcineurin A antibody (ab240913) at 1/1000 dilution + Rat brain lysate at 15 µg
Secondary
HRP-linked goat anti-rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Exposure time: 5 minutesBlocked with 5% skim milk in 1XTBST.
Primary incubation: 2 hours at RT.
Secondary incubation: 1 hour RT.
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Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody (ab240913)HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Calcineurin A (green) using ab240913 at 1/120 dilution in ICC/IF, followed by a FITC conjugated goat anti-rabbit IgG at 1/200 dilution. Cells were fixed using 2% formaldehyde for 20 minutes at room temperature. Primary antibody was incubated for 12 hours at 4°C and secondary antibody for 2 hours at room temperature. Counterstained with DAPI (blue) at a 1/40000 dilution, for 2 hours at room temperature.
(A) DAPI (blue)
(B) ab240913 labeling Calcineurin A (green)
(C) Composite.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody (ab240913)Mouse backskin tissue fixed with Bouin's fixative solution, stained for Calcineurin A using ab240913 at 1/100 dilution (1 hour at RT) in immunohistochemical analysis. Secondary antibody was a FITC cnjugated goat anti-rabbit IgG (green) at a 1/50 dilution incubated for 1 hour at RT.
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Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody (ab240913)HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Calcineurin A (green) using ab240913 at 1/120 dilution in ICC/IF, followed by a APC conjugated goat anti-rabbit IgG at 1/200 dilution. Cells were fixed using 2% formaldehyde for 20 minutes at room temperature. Primary antibody was incubated for 12 hours at 4°C and secondary antibody for 2 hours at room temperature. Counterstained with DAPI (blue) at a 1/40000 dilution, for 2 hours at room temperature.
(A) DAPI (blue)
(B) ab240913 labeling Calcineurin A (red)
(C) Composite.
Datasheets and documents
References
ab240913 has not yet been referenced specifically in any publications.
