Overview

  • Product name
    Anti-Calcineurin A antibody
    See all Calcineurin A primary antibodies
  • Description
    Rabbit polyclonal to Calcineurin A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length Calcineurin A fusion expressed in E. coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab3673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
WB 1/2000 - 1/5000. Detects a band of approximately 62 kDa.
ICC/IF 1/500.

Target

  • Function
    Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.
  • Sequence similarities
    Belongs to the PPP phosphatase family. PP-2B subfamily.
  • Cellular localization
    Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha isoform formerly PPP2B antibody
    • Calcineurin A alpha antibody
    • Calcineurin A1 antibody
    • CalcineurinA antibody
    • Calmodulin dependent calcineurin A subunit alpha isoform antibody
    • Calmodulin-dependent calcineurin A subunit alpha isoform antibody
    • CALN antibody
    • CALNA 1 antibody
    • CALNA antibody
    • CALNA1 antibody
    • CAM PRP catalytic subunit antibody
    • CAM-PRP catalytic subunit antibody
    • CCN 1 antibody
    • CCN1 antibody
    • CNA 1 antibody
    • CNA alpha antibody
    • CNA antibody
    • CNA1 antibody
    • PP2BA_HUMAN antibody
    • PPP2B antibody
    • Ppp3ca antibody
    • Protein phosphatase 2B catalytic subunit antibody
    • Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody
    • Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody
    • Protein phosphatase 3 catalytic subunit alpha isozyme antibody
    • Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody
    • Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody
    see all

Images

  • All lanes : Anti-Calcineurin A antibody (ab3673) at 1/200 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size: 62 kDa
    why is the actual band size different from the predicted?

  • Western Blot of wild type mouse brain homogenate with ab3673 used at 1/6000. Please note that this image was assembled from two different blots. (The numbers on the scale correspond to kD).

  • Anti-Calcineurin A antibody (ab3673) at 1/1000 dilution + Lysate prepared from human HUH-7 cells at 15000 cells

    Secondary
    HRP conjugated sheep polyclonal to rabbit IgG at 1/15000 dilution

    Performed under reducing conditions.

    Observed band size: 62 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

  • Immunofluorescence analysis of rat dissociated hippocampal cells, staining Calcineurin A with ab3673.

    Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 and blocked with 10% normal goat serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/500 in 2% normal goat serum) for 1 hour at 23°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Chetsawang J  et al. Methamphetamine toxicity-induced calcineurin activation, nuclear translocation of nuclear factor of activated T-cells and elevation of cyclooxygenase 2 levels are averted by calpastatin overexpression in neuroblastoma SH-SY5Y cells. Neurotoxicology 67:287-295 (2018). Read more (PubMed: 29944913) »
  • Ouyang J  et al. Food restriction induces synaptic incorporation of calcium-permeable AMPA receptors in nucleus accumbens. Eur J Neurosci 45:826-836 (2017). Read more (PubMed: 28112453) »
See all 9 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Answer

Thank you for your interest.
Would you be so kind to provide a summary of the project (sample type, species, application, controls etc) you wish to use this antibody?
I would also advise you to visit the following site where the Terms and Conditions of our testing initiative can be found at: www.abcam.com/collaborationdiscount.
I look forward to hearing from you soon.

Read More

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as expected and for the inconvenience this has caused. As requested, I have issued a free of charge replacement from a different lot with the number FOCR PO-15281 in Order 1206011

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Answer

Thank you for contacting us.

I can confirm that the Anti-Calcineurin A antibody (ab3673) is a ployclonal antibody. The affinity of these types of antibodies will change with different rounds of immunisation as well as different hosts. With different host even the epitopes of recognition might change. For that reason it is not unusual that with another lot the detection of the cleavage product might change.

We would be pleased to make an exception in this special case and offer you another lot of this product to try it with your samples. Please note that we cannot guarantee that this lot will detect the cleavage product the same as the first lot you purchased.

I am sorry that I can not be of more help then that on this occasion. I look forward to hearing from you with details of how you would like to proceed.


Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Dissociated hippocampal cells)
Specification
Dissociated hippocampal cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Triton 5min RT
Blocking step
Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

Dr. Matthew Grubb

Verified customer

Submitted Oct 24 2012

Answer

DISCOUNT CODE: 100%ABR-EXXXX
Expiration date: 25/01/2013
I am very pleased to hear you would like to accept our offer and test ab3673 in ICC in rat hippocampal cells.
This code will give you: 1 free PRIMARY ANTIBODY/PROTEIN OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for ICC in rat hippocampal cells and include this code in the “Additional Comments” section so we know the Abreview is for this promotion.
Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your response.
Could you please confirm if the cells are human or murine origin?
As I understand you wish to test this antibody in immunocytochemistry and not immunohistochemistry. What method will be used to dissociate the hippocampal neurons and for fixating them? Enzymatic or other techniques?
It is important for us and for our customer to distinguish between these two applications Immunocytochemistry (ICC) and immunohistochemistry (IHC-P or IHC-Fr).
I look forward to hearing from you soon.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse liver)
Loading amount
20 µg
Specification
mouse liver
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 05 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (vero)
Total protein in input
1e+006 cells
Specification
vero
Immuno-precipitation step
Protein A

Abcam user community

Verified customer

Submitted Nov 24 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (huh-7 cells)
Specification
huh-7 cells
Treatment
viral protein expressed
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 minute(s) · Concentration: 0.5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 08 2009

Answer

Thank you for your enquiry. Further to correspondence with the lab I can tell you that the immunogen used for this antibody was a full length calcineurin A protein. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up