Product nameAnti-Calcineurin A antibody
See all Calcineurin A primary antibodies
DescriptionRabbit polyclonal to Calcineurin A
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
A synthetic peptide corresponding to a region between residue 470 and the C-terminus (residue 521) of human Calcineurin A (NP_000935.1).
- Whole cell lysate from hela cells, NIH3T3 cells and 293T cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab71149 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionCalcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-2B subfamily.
Cellular localizationNucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
- Information by UniProt
- Alpha isoform formerly PPP2B antibody
- Calcineurin A alpha antibody
- Calcineurin A1 antibody
All lanes : Anti-Calcineurin A antibody (ab71149) at 0.1 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg
Predicted band size: 59 kDa
Observed band size: 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Calcineurin A with ab71149 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation/ Western Blot of Calcineurin A
Lane 1: ab71149 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Subsequent WB detection was performed with 1 µg/ml ab71149.
Chemiluminescence with an exposure time of 30 seconds.
This product has been referenced in:
- Haryuna TS et al. Curcumin Reduces the Noise-Exposed Cochlear Fibroblasts Apoptosis. Int Arch Otorhinolaryngol 20:370-376 (2016). IHC ; Rat . Read more (PubMed: 27746842) »
- Thoms KM et al. Cyclosporin A, but not everolimus, inhibits DNA repair mediated by calcineurin: implications for tumorigenesis under immunosuppression. Exp Dermatol 20:232-6 (2011). WB ; Human . Read more (PubMed: 21323745) »