Anti-Calcineurin A antibody [EP1669Y] (ab52761)
![Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)](https://www.abcam.com/ps/products/52/ab52761/Images/ab52761-450125-rabbit-monoclonal-ep1669y-to-calcineurin-a-western-blot-hela-lysates.jpg "Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)")
Key features and details
- Rabbit monoclonal [EP1669Y] to Calcineurin A
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Calcineurin A antibody [EP1669Y]
See all Calcineurin A primary antibodies -
Description
Rabbit monoclonal [EP1669Y] to Calcineurin A -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Hamster
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, SH-SY5Y and HAP1 cell lysates. Flow Cyt (intra): HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1669Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | (3) |
1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
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| Notes |
|---|
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). |
Target
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Function
Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1. -
Sequence similarities
Belongs to the PPP phosphatase family. PP-2B subfamily. -
Cellular localization
Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle. - Information by UniProt
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Database links
- Entrez Gene: 5530 Human
- Entrez Gene: 19055 Mouse
- Entrez Gene: 24674 Rat
- Omim: 114105 Human
- SwissProt: Q08209 Human
- SwissProt: P63328 Mouse
- SwissProt: P63329 Rat
- Unigene: 435512 Human
see all -
Alternative names
- Alpha isoform formerly PPP2B antibody
- Calcineurin A alpha antibody
- Calcineurin A1 antibody
see all
Images
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Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)All lanes : Anti-Calcineurin A antibody [EP1669Y] (ab52761) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PPP3CA knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 59 kDaLanes 1- 2: Merged signal (red and green). Green - ab52761 observed at 59 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab52761 was shown to react with Calcineurin A in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265130 (knockout cell lysate ab257181) was used. Wild-type HeLa and PPP3CA knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab52761 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry (Intracellular) - Anti-Calcineurin A antibody [EP1669Y] (ab52761)](https://www.abcam.com/ps/products/52/ab52761/Images/ab52761-65191-anti-calcineurin-a-antibody-ep1669y-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Calcineurin A antibody [EP1669Y] (ab52761)Overlay histogram showing HeLa cells stained with ab52761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52761, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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![Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)](https://www.abcam.com/ps/products/52/ab52761/Images/ab52761-266696-anti-calcineurin-a-antibody-ep1669y-western-blot.jpg)
Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Calcineurin A knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: SH-SY5Y cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab52761 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab52761 was shown to specifically react with Calcineurin A when Calcineurin A knockout samples were used. Wild-type and Calcineurin A knockout samples were subjected to SDS-PAGE. ab52761 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)](https://www.abcam.com/ps/products/52/ab52761/Images/ab52761-65192-anti-calcineurin-a-antibody-ep1669y-western-blot.jpg)
Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)Anti-Calcineurin A antibody [EP1669Y] (ab52761) at 1/10000 dilution + Hela cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab52761 has been referenced in 7 publications.
- Yang X et al. The Protective Effect of Qishen Granule on Heart Failure after Myocardial Infarction through Regulation of Calcium Homeostasis. Evid Based Complement Alternat Med 2020:1868974 (2020). PubMed: 33149749
- Liu Y et al. C1222C Deletion in Exon 8 of ABL1 Is Involved in Carcinogenesis and Cell Cycle Control of Colorectal Cancer Through IRS1/PI3K/Akt Pathway. Front Oncol 10:1385 (2020). PubMed: 32850446
- Shi W et al. Histone deacetylase 4 mediates high glucose-induced podocyte apoptosis via upregulation of calcineurin. Biochem Biophys Res Commun 533:1061-1068 (2020). PubMed: 33019979
- Fraseur JG & Kinzer-Ursem TL Next generation calmodulin affinity purification: Clickable calmodulin facilitates improved protein purification. PLoS One 13:e0197120 (2018). PubMed: 29864125
- Leifheit-Nestler M et al. Impact of Altered Mineral Metabolism on Pathological Cardiac Remodeling in Elevated Fibroblast Growth Factor 23. Front Endocrinol (Lausanne) 9:333 (2018). PubMed: 29977226
- Chen Y et al. Lercanidipine attenuates angiotensin II-induced cardiomyocyte hypertrophy by blocking calcineurin-NFAT3 and CaMKII-HDAC4 signaling. Mol Med Rep 16:4545-4552 (2017). PubMed: 28849081
- Moreira-Gonçalves D et al. Intermittent cardiac overload results in adaptive hypertrophy and provides protection against left ventricular acute pressure overload insult. J Physiol 593:3885-97 (2015). PubMed: 26010517