Product nameAnti-Calcineurin A antibody [EP1669Y]
See all Calcineurin A primary antibodies
DescriptionRabbit monoclonal [EP1669Y] to Calcineurin A
SpecificityThis antibody reacts with Calcineurin A.
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Hamster, Human
Synthetic peptide within Human Calcineurin A (N terminal). The exact sequence is proprietary.
- WB: HeLa whole cell lysate (ab150035).
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab52761 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCalcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.
Sequence similaritiesBelongs to the PPP phosphatase family. PP-2B subfamily.
Cellular localizationNucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
- Information by UniProt
- Alpha isoform formerly PPP2B antibody
- Calcineurin A alpha antibody
- Calcineurin A1 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Calcineurin A knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab52761 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab52761 was shown to specifically react with Calcineurin A when Calcineurin A knockout samples were used. Wild-type and Calcineurin A knockout samples were subjected to SDS-PAGE. ab52761 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Anti-Calcineurin A antibody [EP1669Y] (ab52761) at 1/10000 dilution + Hela cell lysate at 10 µg
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDa
Overlay histogram showing HeLa cells stained with ab52761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52761, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This product has been referenced in:
- Fraseur JG & Kinzer-Ursem TL Next generation calmodulin affinity purification: Clickable calmodulin facilitates improved protein purification. PLoS One 13:e0197120 (2018). Read more (PubMed: 29864125) »
- Leifheit-Nestler M et al. Impact of Altered Mineral Metabolism on Pathological Cardiac Remodeling in Elevated Fibroblast Growth Factor 23. Front Endocrinol (Lausanne) 9:333 (2018). Read more (PubMed: 29977226) »