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    calcineurin-a-antibody-ep1669y-ab52761.pdf

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Immunology Adaptive Immunity B Cells Non-CD
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Validated using a knockout cell lineRabMAb

Anti-Calcineurin A antibody [EP1669Y] (ab52761)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (7)

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Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)
  • Flow Cytometry (Intracellular) - Anti-Calcineurin A antibody [EP1669Y] (ab52761)
  • Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)
  • Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)

Key features and details

  • Rabbit monoclonal [EP1669Y] to Calcineurin A
  • Suitable for: Flow Cyt (Intra), WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

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Recombinant Human Calcineurin A protein (ab101870)
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Overview

  • Product name

    Anti-Calcineurin A antibody [EP1669Y]
    See all Calcineurin A primary antibodies
  • Description

    Rabbit monoclonal [EP1669Y] to Calcineurin A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Hamster
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, SH-SY5Y and HAP1 cell lysates. Flow Cyt (intra): HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1669Y
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • Non-CD
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calmodulin Pathway

Associated products

  • Alternative Versions

    • Anti-Calcineurin A antibody [EP1669Y] - BSA and Azide free (ab239848)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human PPP3CA (Calcineurin A) knockout HeLa cell line (ab265130)
  • KO cell lysates

    • Human PPP3CA (Calcineurin A) knockout HeLa cell lysate (ab257181)
  • Recombinant Protein

    • Recombinant Human Calcineurin A protein (ab101870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (3)
1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
Notes
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
Application notes
Is unsuitable for ICC/IF or IHC-P.

Target

  • Function

    Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.
  • Sequence similarities

    Belongs to the PPP phosphatase family. PP-2B subfamily.
  • Cellular localization

    Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
  • Target information above from: UniProt accession Q08209 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5530 Human
    • Entrez Gene: 19055 Mouse
    • Entrez Gene: 24674 Rat
    • Omim: 114105 Human
    • SwissProt: Q08209 Human
    • SwissProt: P63328 Mouse
    • SwissProt: P63329 Rat
    • Unigene: 435512 Human
    • Unigene: 331389 Mouse
    • Unigene: 6866 Rat
    see all
  • Alternative names

    • Alpha isoform formerly PPP2B antibody
    • Calcineurin A alpha antibody
    • Calcineurin A1 antibody
    • CalcineurinA antibody
    • Calmodulin dependent calcineurin A subunit alpha isoform antibody
    • Calmodulin-dependent calcineurin A subunit alpha isoform antibody
    • CALN antibody
    • CALNA 1 antibody
    • CALNA antibody
    • CALNA1 antibody
    • CAM PRP catalytic subunit antibody
    • CAM-PRP catalytic subunit antibody
    • CCN 1 antibody
    • CCN1 antibody
    • CNA 1 antibody
    • CNA alpha antibody
    • CNA antibody
    • CNA1 antibody
    • PP2BA_HUMAN antibody
    • PPP2B antibody
    • Ppp3ca antibody
    • Protein phosphatase 2B catalytic subunit antibody
    • Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody
    • Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody
    • Protein phosphatase 3 catalytic subunit alpha isozyme antibody
    • Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody
    • Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody
    see all

Images

  • Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)
    Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)
    All lanes : Anti-Calcineurin A antibody [EP1669Y] (ab52761) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PPP3CA knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa



    Lanes 1- 2: Merged signal (red and green). Green - ab52761 observed at 59 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab52761 was shown to react with Calcineurin A in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265130 (knockout cell lysate ab257181) was used. Wild-type HeLa and PPP3CA knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab52761 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry (Intracellular) - Anti-Calcineurin A antibody [EP1669Y] (ab52761)
    Flow Cytometry (Intracellular) - Anti-Calcineurin A antibody [EP1669Y] (ab52761)

    Overlay histogram showing HeLa cells stained with ab52761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52761, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. 

     

     

  • Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)
    Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Calcineurin A knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: SH-SY5Y cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab52761 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab52761 was shown to specifically react with Calcineurin A when Calcineurin A knockout samples were used. Wild-type and Calcineurin A knockout samples were subjected to SDS-PAGE. ab52761 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)
    Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)
    Anti-Calcineurin A antibody [EP1669Y] (ab52761) at 1/10000 dilution + Hela cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 59 kDa
    Observed band size: 59 kDa

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (7)

Publishing research using ab52761? Please let us know so that we can cite the reference in this datasheet.

ab52761 has been referenced in 7 publications.

  • Yang X  et al. The Protective Effect of Qishen Granule on Heart Failure after Myocardial Infarction through Regulation of Calcium Homeostasis. Evid Based Complement Alternat Med 2020:1868974 (2020). PubMed: 33149749
  • Liu Y  et al. C1222C Deletion in Exon 8 of ABL1 Is Involved in Carcinogenesis and Cell Cycle Control of Colorectal Cancer Through IRS1/PI3K/Akt Pathway. Front Oncol 10:1385 (2020). PubMed: 32850446
  • Shi W  et al. Histone deacetylase 4 mediates high glucose-induced podocyte apoptosis via upregulation of calcineurin. Biochem Biophys Res Commun 533:1061-1068 (2020). PubMed: 33019979
  • Fraseur JG & Kinzer-Ursem TL Next generation calmodulin affinity purification: Clickable calmodulin facilitates improved protein purification. PLoS One 13:e0197120 (2018). PubMed: 29864125
  • Leifheit-Nestler M  et al. Impact of Altered Mineral Metabolism on Pathological Cardiac Remodeling in Elevated Fibroblast Growth Factor 23. Front Endocrinol (Lausanne) 9:333 (2018). PubMed: 29977226
  • Chen Y  et al. Lercanidipine attenuates angiotensin II-induced cardiomyocyte hypertrophy by blocking calcineurin-NFAT3 and CaMKII-HDAC4 signaling. Mol Med Rep 16:4545-4552 (2017). PubMed: 28849081
  • Moreira-Gonçalves D  et al. Intermittent cardiac overload results in adaptive hypertrophy and provides protection against left ventricular acute pressure overload insult. J Physiol 593:3885-97 (2015). PubMed: 26010517

Customer reviews and Q&As

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1-3 of 3 Abreviews

Western blot abreview for Anti-Calcineurin A antibody [EP1669Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Tissue lysate - whole (CNS tissue)
Specification
CNS tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 01 2014

Western blot abreview for Anti-Calcineurin A antibody [EP1669Y]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
75 µg
Gel Running Conditions
Reduced Denaturing (15)
Sample
Mouse Tissue lysate - whole (brain Tissue)
Specification
brain Tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 13 2014

Western blot abreview for Anti-Calcineurin A antibody [EP1669Y]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Hamster Cell lysate - whole cell (CHO)
Loading amount
5 µg
Specification
CHO
Gel Running Conditions
Reduced Denaturing (10-20% tris-glycine gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 13 2008

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