Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
- Datasheet
- Specific References (1)
- Protocols
Overview
-
Product nameAnti-Calcineurin A antibody [EPR1670(2)]
See all Calcineurin A primary antibodies -
DescriptionRabbit monoclonal [EPR1670(2)] to Calcineurin A
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide within Human Calcineurin A aa 500-600 (C terminal). The exact sequence is proprietary.
-
Positive control
- Fetal brain, SH-SY5Y, A431, and HeLa cell lysates; HeLa cells.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
-
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR1670(2)
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab109412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 58 kDa (predicted molecular weight: 59 kDa). | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
|
| ICC/IF | 1/100 - 1/250. |
Target
-
FunctionCalcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.
-
Sequence similaritiesBelongs to the PPP phosphatase family. PP-2B subfamily.
-
Cellular localizationNucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
- Information by UniProt
-
Database links
- Entrez Gene: 5530 Human
- Entrez Gene: 19055 Mouse
- Entrez Gene: 24674 Rat
- Omim: 114105 Human
- SwissProt: Q08209 Human
- SwissProt: P63328 Mouse
- SwissProt: P63329 Rat
- Unigene: 435512 Human
see all -
Alternative names
- Alpha isoform formerly PPP2B antibody
- Calcineurin A alpha antibody
- Calcineurin A1 antibody
see all
Images
-
![Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)](https://www.abcam.com/ps/products/109/ab109412/Images/ab109412-266697-ab109412KOWB.jpg)
Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Calcineurin A knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab109412 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109412 was shown to specifically react with Calcineurin A when Calcineurin A knockout samples were used. Wild-type and Calcineurin A knockout samples were subjected to SDS-PAGE. ab109412 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
![Flow Cytometry - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)](https://www.abcam.com/ps/products/109/ab109412/Images/ab109412-3-ab109412FC.jpg)
Flow Cytometry - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)Overlay histogram showing HeLa cells stained with ab109412 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109412, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)](https://www.abcam.com/ps/products/109/ab109412/Images/Calcineurin-A-Primary-antibodies-ab109412-2.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)Immunofluorescent staining of HeLa cells using 1/100 ab109412. -
![Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)](https://www.abcam.com/ps/products/109/ab109412/Images/Calcineurin-A-Primary-antibodies-ab109412-1.JPG)
Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)All lanes : Anti-Calcineurin A antibody [EPR1670(2)] (ab109412) at 1/10000 dilution
Lane 1 : fetal brain lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : A431 lysate
Lane 4 : HeLa cells lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 59 kDa
Observed band size: 58 kDa (why is the actual band size different from the predicted?)
Protocols
References
This product has been referenced in:
- Tian CJ & Pang X Ca2+-calcineurin signaling is involved in norepinephrine-induced cardiac fibroblasts activation. Int J Clin Exp Pathol 8:5210-6 (2015). Read more (PubMed: 26191219) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"