Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1670(2)] to Calcineurin A
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Calcineurin A antibody [EPR1670(2)]
    See all Calcineurin A primary antibodies

  • Description

    Rabbit monoclonal [EPR1670(2)] to Calcineurin A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Calcineurin A aa 500-600 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Fetal brain, SH-SY5Y, A431, and HeLa cell lysates; HeLa cells. Mouse and rat brain cortex lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab109412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 58 kDa (predicted molecular weight: 59 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.

    • Sequence similarities

      Belongs to the PPP phosphatase family. PP-2B subfamily.

    • Cellular localization

      Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Alpha isoform formerly PPP2B antibody
      • Calcineurin A alpha antibody
      • Calcineurin A1 antibody
      • CalcineurinA antibody
      • Calmodulin dependent calcineurin A subunit alpha isoform antibody
      • Calmodulin-dependent calcineurin A subunit alpha isoform antibody
      • CALN antibody
      • CALNA 1 antibody
      • CALNA antibody
      • CALNA1 antibody
      • CAM PRP catalytic subunit antibody
      • CAM-PRP catalytic subunit antibody
      • CCN 1 antibody
      • CCN1 antibody
      • CNA 1 antibody
      • CNA alpha antibody
      • CNA antibody
      • CNA1 antibody
      • PP2BA_HUMAN antibody
      • PPP2B antibody
      • Ppp3ca antibody
      • Protein phosphatase 2B catalytic subunit antibody
      • Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody
      • Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody
      • Protein phosphatase 3 catalytic subunit alpha isozyme antibody
      • Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody
      • Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody
      see all

    Images

    • Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
      Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
      All lanes : Anti-Calcineurin A antibody [EPR1670(2)] (ab109412) at 1/3000 dilution

      Lane 1 : Mouse brain cortex lysate
      Lane 2 : Mouse lung lysate
      Lane 3 : Rat brain cortex lysate
      Lane 4 : Rat liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

      Predicted band size: 59 kDa
      Observed band size: 58 kDa
      why is the actual band size different from the predicted?

    • Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
      Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Calcineurin A knockout HAP1 cell lysate (20 µg)
      Lane 3: A431 cell lysate (20 µg)
      Lane 4: HeLa cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab109412 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109412 was shown to specifically react with Calcineurin A when Calcineurin A knockout samples were used. Wild-type and Calcineurin A knockout samples were subjected to SDS-PAGE. ab109412 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Flow Cytometry - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
      Flow Cytometry - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
      Overlay histogram showing HeLa cells stained with ab109412 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109412, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
      Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
      Immunofluorescent staining of HeLa cells using 1/100 ab109412.
    • Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
      Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
      All lanes : Anti-Calcineurin A antibody [EPR1670(2)] (ab109412) at 1/10000 dilution

      Lane 1 : fetal brain lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : A431 lysate
      Lane 4 : HeLa cells lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 59 kDa
      Observed band size: 58 kDa why is the actual band size different from the predicted?

    • Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
      Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)

    References (1)

    Publishing research using ab109412? Please let us know so that we can cite the reference in this datasheet.

    ab109412 has been referenced in 1 publication.

    • Tian CJ & Pang X Ca2+-calcineurin signaling is involved in norepinephrine-induced cardiac fibroblasts activation. Int J Clin Exp Pathol 8:5210-6 (2015). PubMed: 26191219

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