Overview

  • Product name
    Anti-Calcineurin B antibody
    See all Calcineurin B primary antibodies
  • Description
    Rabbit polyclonal to Calcineurin B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-120 of Human Calcineurin B (UniProt P63098).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, Molt 4 and Raji cell lysates; Human NCI-N87 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 19 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function
    Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
  • Sequence similarities
    Belongs to the calcineurin regulatory subunit family.
    Contains 4 EF-hand domains.
  • Information by UniProt
  • Database links
  • Alternative names
    • alpha isoform (calcineurin B, type I) antibody
    • calcineurin B, type I (19kDa) antibody
    • Calcineurin subunit B type 1 antibody
    • CALNB1 antibody
    • CANB1_HUMAN antibody
    • Cna2 antibody
    • CNB antibody
    • CNB1 antibody
    • OTTHUMP00000201960 antibody
    • OTTHUMP00000201961 antibody
    • Ppp3r1 antibody
    • PPP3R1 protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform antibody
    • protein phosphatase3 (formerly2B), regulatory subunit B, alpha isoform antibody
    • Protein phosphatase 2B regulatory subunit 1 antibody
    • Protein phosphatase 2B regulatory subunit B alpha antibody
    • protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) antibody
    • Protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform (calcineurin B, type I) antibody
    • Protein phosphatase 3 regulatory subunit B alpha antibody
    • Protein phosphatase 3 regulatory subunit B alpha isoform 1 antibody
    see all

Images

  • All lanes : Anti-Calcineurin B antibody (ab154650) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa whole cell lysate
    Lane 5 : Hep G2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 19 kDa



    15% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue labeling Calcineurin B with ab154650 at 1/500 dilution.

References

This product has been referenced in:
  • Marciel MP  et al. Selenoprotein K deficiency inhibits melanoma by reducing calcium flux required for tumor growth and metastasis. Oncotarget 9:13407-13422 (2018). Read more (PubMed: 29568366) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (CNS)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
CNS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 31 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (CNS)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
CNS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 31 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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