Key features and details
- Rabbit polyclonal to Calcineurin B/CNB
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Calcineurin B/CNB antibody
See all Calcineurin B/CNB primary antibodies
DescriptionRabbit polyclonal to Calcineurin B/CNB
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
- WB: SH-SY5Y, SW480, HL-60 and MCF7 cell extracts; mouse brain, spleen and lung cell extracts; rat brain and testis cell extracts. IHC-P: Human brain cortex tissue.
This product was previously labelled as Calcineurin B
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Concentration information loading...
- Mouse spleen tissue lysate - total protein (ab29293)
- Mouse lung normal tissue lysate - total protein (ab29297)
- MCF7 whole cell lysate (ab29537)
- Mouse brain tissue lysate - total protein (ab30151)
- MCF7 whole cell lysate (ab3871)
- SW480 whole cell lysate (ab3957)
- Mouse brain tissue lysate - total protein (ab4022)
- HL60 whole cell lysate (ab7914)
- Mouse spleen tissue lysate - total protein (ab7937)
Our Abpromise guarantee covers the use of ab230899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/500 - 1/2000. Predicted molecular weight: 19 kDa.|
FunctionRegulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
Sequence similaritiesBelongs to the calcineurin regulatory subunit family.
Contains 4 EF-hand domains.
- Information by UniProt
- alpha isoform (calcineurin B, type I) antibody
- calcineurin B, type I (19kDa) antibody
- Calcineurin subunit B type 1 antibody
All lanes : Anti-Calcineurin B/CNB antibody (ab230899) at 1/500 dilution
Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell extract
Lane 2 : SW480 (human colorectal adenocarcinoma cell line) cell extract
Lane 3 : HL-60 (human promyelocytic leukemia cell line) cell extract
Lane 4 : MCF7 (human breast adenocarcinoma cell line) cell extract
Lane 5 : Mouse brain cell extract
Lane 6 : Mouse spleen cell extract
Lane 7 : Mouse lung cell extract
Lane 8 : Rat brain cell extract
Lane 9 : Rat testis cell extract
Developed using the ECL technique.
Predicted band size: 19 kDa
Formalin-fixed, paraffin-embedded human brain cortex tissue stained for Calcineurin B/CNB with ab230899 at 1/50 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230899 has not yet been referenced specifically in any publications.