Anti-Calcineurin B/PPP3R1 antibody (ab231630)
Key features and details
- Rabbit polyclonal to Calcineurin B/PPP3R1
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Calcineurin B/PPP3R1 antibody
See all Calcineurin B/PPP3R1 primary antibodies -
Description
Rabbit polyclonal to Calcineurin B/PPP3R1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant full length protein corresponding to Human Calcineurin B/PPP3R1 aa 1 to the C-terminus. (Expressed in E.coli).
Database link: P63098 -
Positive control
- IHC-P: Human cerebrum tissue. WB: Pig stomach lysate; HeLa and A431 cell lysates; Recombinant human Calcineurin B/PPP3R1 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231630 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 19 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 19 kDa. |
Target
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Function
Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity. -
Sequence similarities
Belongs to the calcineurin regulatory subunit family.
Contains 4 EF-hand domains. - Information by UniProt
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Database links
- Entrez Gene: 282321 Cow
- Entrez Gene: 5534 Human
- Entrez Gene: 19058 Mouse
- Entrez Gene: 100627903 Pig
- Entrez Gene: 29748 Rat
- Omim: 601302 Human
- SwissProt: P63099 Cow
- SwissProt: P63098 Human
see all -
Alternative names
- alpha isoform (calcineurin B, type I) antibody
- calcineurin B, type I (19kDa) antibody
- Calcineurin subunit B type 1 antibody
see all
Images
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Paraffin-embedded human cerebrum tissue stained for Calcineurin B/PPP3R1 using ab231630 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human cerebrum tissue stained for Calcineurin B/PPP3R1 using ab231630 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-Calcineurin B/PPP3R1 antibody (ab231630) at 3 µg/ml + Recombinant human Calcineurin B/PPP3R1 protein
Predicted band size: 19 kDa -
All lanes : Anti-Calcineurin B/PPP3R1 antibody (ab231630) at 3 µg/ml
Lane 1 : Pig stomach lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 19 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231630 has not yet been referenced specifically in any publications.