Recombinant Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

Product name

Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18]
See all Calcineurin B/PPP3R1 primary antibodies
Description

Rabbit monoclonal [EPR24992-18] to Calcineurin B/PPP3R1
Host species

Rabbit
Specificity

Unsuitable for human IP
Tested applications

Suitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt (Intra) or IHC-Fr
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse brain, Mouse kidney, Mouse heart, Rat brain, Rat kidney, Rat heart, SK-N-SH, SH-SY5Y, Neuro-2a, HEK-293T transfected with a human PPP3R1 expression vector containing a his tag, whole cell lysate, and HEK-293T transfected with a human PPP3R2 expression vector containing a his tag, whole cell lysate. IHC-P: Human cerebrum, Mouse cerebrum, Rat cerebrum, Human cardiac muscle, Mouse kidney and Rat cardiac muscle tissues. IP: Mouse brain and Rat brain cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24992-18
Isotype

IgG
Research areas

Alternative Versions
- Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] - BSA and Azide free (ab303483)
- Anti-NPTX2 antibody [EPR27176-81] - BSA and Azide free (ab305258)
Compatible Secondaries
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab303482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Detects a band of approximately 15 kDa.
IHC-P		1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP		1/30.

Notes
WB 1/1000. Detects a band of approximately 15 kDa.
IHC-P 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.

Application notes

Is unsuitable for Flow Cyt (Intra) or IHC-Fr.

Function

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
Sequence similarities

Belongs to the calcineurin regulatory subunit family.
Contains 4 EF-hand domains.
Target information above from: UniProt accession P63098 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 5534 Human
- Entrez Gene: 19058 Mouse
- Entrez Gene: 29748 Rat
- Omim: 601302 Human
- SwissProt: P63098 Human
- SwissProt: Q63810 Mouse
- SwissProt: P63100 Rat
- Unigene: 280604 Human
- Unigene: 41840 Mouse
- Unigene: 42903 Rat
see all
Alternative names
- alpha isoform (calcineurin B, type I) antibody
- calcineurin B, type I (19kDa) antibody
- Calcineurin subunit B type 1 antibody
- CALNB1 antibody
- CANB1_HUMAN antibody
- Cna2 antibody
- CNB antibody
- CNB1 antibody
- OTTHUMP00000201960 antibody
- OTTHUMP00000201961 antibody
- Ppp3r1 antibody
- PPP3R1 protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform antibody
- protein phosphatase3 (formerly2B), regulatory subunit B, alpha isoform antibody
- Protein phosphatase 2B regulatory subunit 1 antibody
- Protein phosphatase 2B regulatory subunit B alpha antibody
- protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform (calcineurin B, type I) antibody
- Protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform (calcineurin B, type I) antibody
- Protein phosphatase 3 regulatory subunit B alpha antibody
- Protein phosphatase 3 regulatory subunit B alpha isoform 1 antibody
see all

Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

All lanes : Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate 20 µg
Lane 2 : Mouse kidney tissue lysate 20 µg
Lane 3 : Mouse heart tissue lysate 20 µg
Lane 4 : Rat brain tissue lysate 20 µg
Lane 5 : Rat kidney tissue lysate 20 µg
Lane 6 : Rat heart tissue lysate 20 µg

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Observed band size: 15 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBSTLow expression: heart, kidney (PMID:8110831).

Exposure time: 10 seconds
Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

All lanes : Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482) at 1/1000 dilution

Lane 1 : SK-N-SH (human neuroblastoma epithelial cell), whole cell lysate 20 µg
Lane 2 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 20 µg
Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 20 µg

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Observed band size: 15 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBSTThis blot was developed using a high sensitivity ECL substrate.

Exposure time: 180 seconds
Western blot - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

All lanes : Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482) at 1/1000 dilution

Lane 1 : HEK-293T cells transfected with a human PPP3R1 expression vector containing a his tag, whole cell lysate, 20 µg
Lane 2 : HEK-293T cells transfected with a human PPP3R2 expression vector containing a his tag, whole cell lysate, 20 µg

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Blocking and diluting buffer and concentration: 5% NFDM/TBSTThis antibody does not cross-react with human PPP3R2.

Exposure time: 50 seconds
Immunoprecipitation - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

Calcineurin B/PPP3R1 was immunoprecipitated from 0.35 mg Rat brain tissue lysate 10 ug with ab303482 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab303482 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat brain tissue lysate 10 ug

Lane 2: abab303482 IP in Rat brain tissue lysate

Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab303482 in rat brain tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 180 seconds

This blot was developed using a high sensitivity ECL substrate.
Immunoprecipitation - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

Calcineurin B/PPP3R1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab303482 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab303482 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10 ug

Lane 2: abab303482 IP in Mouse brain tissue lysate

Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab303482 in mouse brain tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 180 seconds

This blot was developed using a high sensitivity ECL substrate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: No staining on rat cardiac muscle. The section was incubated with ab303482 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: No staining on mouse kidney. The section was incubated with ab303482 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: No staining on human cardiac muscle. The section was incubated with ab303482 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ , Polymer Refine Detection) was used. Positive staining on rat cerebrum. The section was incubated with ab303482 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse cerebrum. The section was incubated with ab303482 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Calcineurin B/PPP3R1 with ab303482 at 1/5000 (0.097 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human cerebrum. The section was incubated with ab303482 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Anti-Calcineurin B/PPP3R1 antibody [EPR24992-18] (ab303482)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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Key features and details

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (0)

Customer reviews and Q&As