Overview

  • Product name

    Anti-Calcineurin inhibitor antibody
    See all Calcineurin inhibitor primary antibodies
  • Description

    Rabbit polyclonal to Calcineurin inhibitor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Calcineurin inhibitor aa 1978-2123.
    Database link: Q9Y6J0

  • Positive control

    • WB: A549 whole cell lysate; Rat brain tissue lysate. IHC-P: Human colon cancer and cervical cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.03% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity greater than 95%.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab238743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/5000.

Target

  • Function

    May be required for replication-independent chromatin assembly. May serve as a negative regulator of T-cell receptor (TCR) signaling via inhibition of calcineurin. Inhibition of activated calcineurin is dependent on both PKC and calcium signals.
  • Tissue specificity

    Widely expressed in different tissues.
  • Sequence similarities

    Contains 6 TPR repeats.
  • Post-translational
    modifications

    Activated through PKC-mediated hyperphosphorylation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • A330070M20Rik antibody
    • Cabin 1 antibody
    • CABIN_HUMAN antibody
    • Cabin1 antibody
    • CAIN antibody
    • Calcineurin binding protein cabin 1 antibody
    • Calcineurin inhibitor antibody
    • Calcineurin-binding protein cabin-1 antibody
    • KIAA0330 antibody
    • PPP3IN antibody
    see all

Images

  • All lanes : Anti-Calcineurin inhibitor antibody (ab238743) at 3.1 µg/ml

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : Rat brain tissue lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
  • Paraffin-embedded human colon cancer tissue stained for Calcineurin inhibitor using ab238743 at 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human cervical cancer tissue stained for Calcineurin inhibitor using ab238743 at 1/400 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

References

ab238743 has not yet been referenced specifically in any publications.

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