• Product name
    Anti-Calcineurin inhibitor antibody
    See all Calcineurin inhibitor primary antibodies
  • Description
    Rabbit polyclonal to Calcineurin inhibitor
  • Host species
  • Tested applications
    Suitable for: ELISA, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein (His-tag) corresponding to Rat Calcineurin inhibitor aa 1881-2173.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: 99% PBS
  • Purity
    Whole antiserum
  • Primary antibody notes
    Calcineurin is a widely distributed Ser/Thr, calcium and calmodulin-dependent protein phosphatase. It mediates the immunosuppressive actions of drugs such as FK506 and cyclosporin, and has also been implicated in a number of calcium-sensitive pathways in the nervous system. Calcineurin has been found to be associated with other proteins, such as calmodulin, FKBP12, the ryanodine receptor, inositol 1,4,5-trisphosphate, and a recently identified protein, calcineurin inhibitor (CAIN). CAIN has been shown to bind calcineurin through its C-terminal tail. Recent studies have shown CAIN to bind to both amphiphysin 1 and calcineurin simultaneously and therefore acts as a physiological inhibitor of calcineurin. The binding of CAIN to amphiphysin 1 does not affect the interaction of amphiphysin 1 with other endocytic proteins. CAIN, like calcineurin, is highly expressed in neurons and other tissues.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab3349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/15000.
IP Use at an assay dependent concentration.

Immunoprecipitation of CAIN from rat brain tissues shows an interaction between CAIN and amphiphysin 1, adaptin, and dynamin 1.

WB 1/1000.

By Western blot, this antibody detects an ~240 kDa protein representing cain from rat liver extract.

ICC/IF Use at an assay dependent concentration.


  • Function
    May be required for replication-independent chromatin assembly. May serve as a negative regulator of T-cell receptor (TCR) signaling via inhibition of calcineurin. Inhibition of activated calcineurin is dependent on both PKC and calcium signals.
  • Tissue specificity
    Widely expressed in different tissues.
  • Sequence similarities
    Contains 6 TPR repeats.
  • Post-translational
    Activated through PKC-mediated hyperphosphorylation.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • A330070M20Rik antibody
    • Cabin 1 antibody
    • CABIN_HUMAN antibody
    • Cabin1 antibody
    • CAIN antibody
    • Calcineurin binding protein cabin 1 antibody
    • Calcineurin inhibitor antibody
    • Calcineurin-binding protein cabin-1 antibody
    • KIAA0330 antibody
    • PPP3IN antibody
    see all


  • ICC/IF image of ab3349 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3349, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Zhang L  et al. Downregulation of miRNA-15a and miRNA-16 promote tumor proliferation in multiple myeloma by increasing CABIN1 expression. Oncol Lett 15:1287-1296 (2018). Read more (PubMed: 29399181) »
  • Ray-Gallet D  et al. Functional activity of the H3.3 histone chaperone complex HIRA requires trimerization of the HIRA subunit. Nat Commun 9:3103 (2018). Read more (PubMed: 30082790) »
See all 5 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Frozen sections)
Mouse Tissue sections (heart)
Yes - 0.3% Triton X
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 11 2015

Western blot
Mouse Tissue lysate - whole (Whole Embryo)
Gel Running Conditions
Reduced Denaturing (4%-20%)
Whole Embryo
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 13 2013


I am very pleased to hear you would like to accept our offer and test ab3349 in mouse. This code will give you: 1 free PRIMARY ANTIBODY OR COST OF PRODUCT OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview formouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Thank you for contacting us. As discussed on the phone this antibody stock is whole serum it is therefore difficult to determine the concentration of the antibody, since whole serum contains many other host serum proteins besides the antibody of interest. The amount of anti-CAIN IgG in the antiserum for ab3349 has never been determined. In general terms rabbit serum such as this generally contains 1 to 10 mg/ml of specific IgG. For every application used, optimisation of the antibody dilution will need to be performed but as I starting point I would suggest the following: WB: 1/500 IHC/ICC: 1/50-1/100 ELISA: 1/500 FACS/flow cytometry: 1/500 IP: 1/50-1/100 Unfortunately I do not have any more specific information. If you have any further questions please do not heistate to ask.

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Thank you for contacting us. We are sorry; ab57137 is no longer included in our product range. We apologize for the inconvenience caused. Could we recommend few other products from our catalogue? We have ab3349 and ab76600 antibodies available to buy. ab76600; the discount code can be provided for IP, ICC/IF or Dog species. ab3349; the discount code can be provided for Dog species only. We also have peptide ab88931 that can be used in studies with ab76600 as blocking peptide. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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