Overview

  • Product name
    Anti-Calcineurin inhibitor antibody
    See all Calcineurin inhibitor primary antibodies
  • Description
    Rabbit polyclonal to Calcineurin inhibitor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISA, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein (His-tag) corresponding to Rat Calcineurin inhibitor aa 1881-2173.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: 99% PBS
  • Purity
    Whole antiserum
  • Primary antibody notes
    Calcineurin is a widely distributed Ser/Thr, calcium and calmodulin-dependent protein phosphatase. It mediates the immunosuppressive actions of drugs such as FK506 and cyclosporin, and has also been implicated in a number of calcium-sensitive pathways in the nervous system. Calcineurin has been found to be associated with other proteins, such as calmodulin, FKBP12, the ryanodine receptor, inositol 1,4,5-trisphosphate, and a recently identified protein, calcineurin inhibitor (CAIN). CAIN has been shown to bind calcineurin through its C-terminal tail. Recent studies have shown CAIN to bind to both amphiphysin 1 and calcineurin simultaneously and therefore acts as a physiological inhibitor of calcineurin. The binding of CAIN to amphiphysin 1 does not affect the interaction of amphiphysin 1 with other endocytic proteins. CAIN, like calcineurin, is highly expressed in neurons and other tissues.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/15000.
IP Use at an assay dependent concentration.

Immunoprecipitation of CAIN from rat brain tissues shows an interaction between CAIN and amphiphysin 1, adaptin, and dynamin 1.

WB 1/1000.

By Western blot, this antibody detects an ~240 kDa protein representing cain from rat liver extract.

ICC/IF Use at an assay dependent concentration.

Target

  • Function
    May be required for replication-independent chromatin assembly. May serve as a negative regulator of T-cell receptor (TCR) signaling via inhibition of calcineurin. Inhibition of activated calcineurin is dependent on both PKC and calcium signals.
  • Tissue specificity
    Widely expressed in different tissues.
  • Sequence similarities
    Contains 6 TPR repeats.
  • Post-translational
    modifications
    Activated through PKC-mediated hyperphosphorylation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • A330070M20Rik antibody
    • Cabin 1 antibody
    • CABIN_HUMAN antibody
    • Cabin1 antibody
    • CAIN antibody
    • Calcineurin binding protein cabin 1 antibody
    • Calcineurin inhibitor antibody
    • Calcineurin-binding protein cabin-1 antibody
    • KIAA0330 antibody
    • PPP3IN antibody
    see all

Images

  • ICC/IF image of ab3349 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3349, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Zhang L  et al. Downregulation of miRNA-15a and miRNA-16 promote tumor proliferation in multiple myeloma by increasing CABIN1 expression. Oncol Lett 15:1287-1296 (2018). Read more (PubMed: 29399181) »
  • Ray-Gallet D  et al. Functional activity of the H3.3 histone chaperone complex HIRA requires trimerization of the HIRA subunit. Nat Commun 9:3103 (2018). Read more (PubMed: 30082790) »
See all 5 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Permeabilization
Yes - 0.3% Triton X
Specification
heart
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 11 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole Embryo)
Gel Running Conditions
Reduced Denaturing (4%-20%)
Specification
Whole Embryo
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Aug 13 2013

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